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The map label for this gene is alsB [H]

Identifier: 161524804

GI number: 161524804

Start: 1807676

End: 1808626

Strand: Reverse

Name: alsB [H]

Synonym: Bmul_1631

Alternate gene names: 161524804

Gene position: 1808626-1807676 (Counterclockwise)

Preceding gene: 161524805

Following gene: 161524803

Centisome position: 52.45

GC content: 65.09

Gene sequence:

>951_bases
ATGAATGTCCGCTTTCGCCGTCGCTTTCTGACCGCCGCGCTCGCTGCCGTCGCGGTTGCCGCCGCTCCCGCCGTCCACGC
GCAAGCGGCCGGCAAGCCGAAGGTCGCGCTCGTGATGAAGTCGCTCGCCAACGAGTTCTTCCTGACGATGGAAAACGGCG
CGAAGGAATACCAGAAACACAACGCGAGCCAGTTCGACCTGATCACGAACGGGATCAAGGATGAGACCGATACCGCGAAC
CAGATCCGCATCGTCGAGCAGATGATCGTGTCGAAGGTCGACGCGATCGTGCTCGCGCCGGCCGACTCGAAGGCGCTCGT
GCCGGTCGTGAAGAAGGCCGTCGACGCGGGCATCATCGTCGTCAACATCGACAACCGGCTCGATCCCGACGTGCTGAAGT
CGAAGAACCTCAACGTGCCGTTCGTCGGGCCCGACAACCGCAAGGGCGCGCGCAAGGTCGGCGACTACCTCGCGAAGAAG
CTGAAGGCGGGCGACCAGGTCGGCATCGTCGAAGGCGTGTCGACGACGACCAACGCGCAGCAGCGCACGGCCGGCTTCCA
GGACGCGATGAAGGCGGGCGGCATGAAGGTCGTGTCGGTGCAATCGGGCGAATGGGAAATCGACAAGGGCAACGCGGTCG
CGTCCGCGATGCTGAACGAATACCCGAACCTGAAGGCGCTGCTGTGCGGCAACGACAACATGGCGATCGGCGCCGTGTCG
GCCGTGCGCGCGGCCGGCAAGCAGGGCAAGGTGTACGTGGTCGGCTACGACAACATCAACGCGATCAAGCCGATGCTGAA
GGACGGCCGCGTGCTCGCGACGGCCGATCAGTACGCCGCGAAGCAGGCCGTGTTCGGCATCGACACGGCGCTGAAGGCGA
TCGCCGAGCACCGCAAGCAGGCGGAGCTGTCGGGCGTGGTCGAGACGCCGGTCGATCTCGTGACGAAGTGA

Upstream 100 bases:

>100_bases
CAAGCAGCATCGATGGCCAATCGCCGGGTAGTGTAGCGCAAACGTTTGCTCGCAATAAAACAACAGTCGGCCCCGACGGG
CCAGTCGCCAGGAGATGTGT

Downstream 100 bases:

>100_bases
CGCCGCGCGGCGCGTGCCGCCGCCCTCAAGAATCCGGCGCGGCGGCCGTTATCCGGTGTGAGCGCGATCACGGCCGCTGC
ATGCCGATGCAGTTGTGAGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm]; D-allose [Cytoplasm] [C]

Alternate protein names: ALBP [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNVRFRRRFLTAALAAVAVAAAPAVHAQAAGKPKVALVMKSLANEFFLTMENGAKEYQKHNASQFDLITNGIKDETDTAN
QIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKNLNVPFVGPDNRKGARKVGDYLAKK
LKAGDQVGIVEGVSTTTNAQQRTAGFQDAMKAGGMKVVSVQSGEWEIDKGNAVASAMLNEYPNLKALLCGNDNMAIGAVS
AVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDTALKAIAEHRKQAELSGVVETPVDLVTK

Sequences:

>Translated_316_residues
MNVRFRRRFLTAALAAVAVAAAPAVHAQAAGKPKVALVMKSLANEFFLTMENGAKEYQKHNASQFDLITNGIKDETDTAN
QIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKNLNVPFVGPDNRKGARKVGDYLAKK
LKAGDQVGIVEGVSTTTNAQQRTAGFQDAMKAGGMKVVSVQSGEWEIDKGNAVASAMLNEYPNLKALLCGNDNMAIGAVS
AVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDTALKAIAEHRKQAELSGVVETPVDLVTK
>Mature_316_residues
MNVRFRRRFLTAALAAVAVAAAPAVHAQAAGKPKVALVMKSLANEFFLTMENGAKEYQKHNASQFDLITNGIKDETDTAN
QIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKNLNVPFVGPDNRKGARKVGDYLAKK
LKAGDQVGIVEGVSTTTNAQQRTAGFQDAMKAGGMKVVSVQSGEWEIDKGNAVASAMLNEYPNLKALLCGNDNMAIGAVS
AVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDTALKAIAEHRKQAELSGVVETPVDLVTK

Specific function: Part of the binding-protein-dependent transport system AlsBAC for D-allose [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790526, Length=255, Percent_Identity=34.9019607843137, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790192, Length=297, Percent_Identity=31.6498316498317, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790674, Length=205, Percent_Identity=33.1707317073171, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788898, Length=318, Percent_Identity=27.0440251572327, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33566; Mature: 33566

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRFRRRFLTAALAAVAVAAAPAVHAQAAGKPKVALVMKSLANEFFLTMENGAKEYQKH
CCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHC
NASQFDLITNGIKDETDTANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIV
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCEEE
VNIDNRLDPDVLKSKNLNVPFVGPDNRKGARKVGDYLAKKLKAGDQVGIVEGVSTTTNAQ
EEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
QRTAGFQDAMKAGGMKVVSVQSGEWEIDKGNAVASAMLNEYPNLKALLCGNDNMAIGAVS
HHHCCHHHHHHHCCEEEEEEECCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHHHH
AVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDTALKAIAEHRKQ
HHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AELSGVVETPVDLVTK
HHHHHHHHCCHHHHCC
>Mature Secondary Structure
MNVRFRRRFLTAALAAVAVAAAPAVHAQAAGKPKVALVMKSLANEFFLTMENGAKEYQKH
CCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHC
NASQFDLITNGIKDETDTANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIV
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCEEE
VNIDNRLDPDVLKSKNLNVPFVGPDNRKGARKVGDYLAKKLKAGDQVGIVEGVSTTTNAQ
EEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
QRTAGFQDAMKAGGMKVVSVQSGEWEIDKGNAVASAMLNEYPNLKALLCGNDNMAIGAVS
HHHCCHHHHHHHCCEEEEEEECCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHHHH
AVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDTALKAIAEHRKQ
HHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AELSGVVETPVDLVTK
HHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; ribose [Periplasm]; D-allose [Periplasm]; ATP [C]

Specific reaction: ATP + H2O + ribose [Periplasm] = ADP + phosphate + ribose [Cytoplasm] D-allose [Periplasm] + ATP + H2O = D-allose [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8576032; 9401019; 10064713 [H]