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The map label for this gene is oprM [H]

Identifier: 161524787

GI number: 161524787

Start: 1782835

End: 1784298

Strand: Reverse

Name: oprM [H]

Synonym: Bmul_1614

Alternate gene names: 161524787

Gene position: 1784298-1782835 (Counterclockwise)

Preceding gene: 161524788

Following gene: 161524785

Centisome position: 51.74

GC content: 71.65

Gene sequence:

>1464_bases
ATGAGGCATCGTTCCGATTCGCGCCGCACCGCGCGGCGCGCGCCGTTCGCGCTGGCGGCCGCGCTCGTGCTGGCCGGCTG
TTCGCTCGCGCCGCGCTACGAGCGTCCGGCTGCGCCGGTGCCGTCCGCGTATACGAACGGCGTCGACGCCACGCACGACG
CGCCGTCGGCTTTCGCGCCGCGCGCCGATGCGGCGCTGCTCGACGACTGGCGCAGCTATTTCACCGCGCCCGCGCTGCAT
GCATGGATCGACGCGGCGCTCGCCAACAATCGCGATCTGCGGATCGCGGCCGGCCGGCTCGACGAGGCGCGCGCGATGTA
CGGCGTGCAGCGTGCGGAGCGGATGCCGTCGATCGACGCGAGCACCGGTTACGAACGCACGCGGCAATACGATCCGGTCC
TGCGCGAGAGCGCGGTGAGCGGGCTGTATCGGGCGGGCGTCGGCATCAGCGCATACGAACTCGATCTGTTCGGGCGCGTG
CGCAATCTGTCCGATGCAGCGCTGGCCGAGTATTTCGCGACGGCCGACGCGCAGCGCACGGTCCGTATCGGCGTGATCGC
CGAAGTCGCGAATGCCTACGTCGCGGAACGCGCGCTGAACGAACAGCGCGCGCTCGCCGAGCGCACGCTCGATGCGCGCG
AACGGATCGCCGCGCTGACGCAACGCCGCTATGCGGCCGGCACGAGCGATGCGATCGAACTGCGTTCGGCCGAGATGCTG
GTCGCGGCCGCGCGTGCATCGCACGCGGCGCTGCAGCGCGAGCACGCGCAGGCCGTGCGCGCGCTGCAACTGCTTGCCGG
CGATTTCGCGCGCGACGTGCGCGACGACGATACGGCGCTCGACGCGCTGACGATCGCGCCGGTCGCGCCCGGTGCGCCGA
GCGATCTGCTCGAGCGACGTCCGGACATCCGGCAGGCCGAGTCGCGGCTCAAGGCCGCGCACGCGCAGATCGGCGCCGCG
CGCGCCGCGTTTTTCCCGCGCATCGCGCTGACGACCGATTACGGCTCGGTCAGCGACGCGTTTTCGAGCCTGTTCGCGGC
CGGCACGAGCGTCTGGACCTTCGCGCCGCGCATCACGCTGCCGATTTTTGCGGGCGGGCGCAATCGCGCGAATCTCGACG
TCGCGAACGCGCGGCGGCACATTGCGGTCGCCGAGTATGAGAAGACCGTGCAGGTCGCGTTCCGCGAGGTCGCGGACGCC
TTTACGGCACGCGACTGGATCGAGCGCCAGCTGGCCGCGCAGCGCGACGTGCACGCGGCCGACGACGCGCGGCTGAAGCT
CGCGGAACGTCGTTACGCGGGCGGCGTCGCGACGTATCTCGAACTGCTCGACGCGCAGCGCAGCACGTACGAATCGGGAC
AGGAGCTGATCCGGCTCAGGCAGCTGCGGCTCGCGAACGCGATCGCGCTGTATCGCGCGCTCGGCGGTGGCTGGACGCCC
GGCACGGCCGCCGACGACGCGTGA

Upstream 100 bases:

>100_bases
TGTTTCTCGTGCCGCTGTTCTTCGTGGTCGTCGGCCGGCTGTTCGGCTTCGGCACGCGCCGACGCGGCAACGCGCCGGCG
GTCAACGTGGAGGGTTCGCG

Downstream 100 bases:

>100_bases
CGCGCTGACGCGTCGTCGCGTCGACAATCGTGCGCCGTTGTCGCTCCGTGCGACAGCGGCGTTTTTTTTCGTCCGCCGGC
CGCGCGGCAGGCGCGCATCG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRHRSDSRRTARRAPFALAAALVLAGCSLAPRYERPAAPVPSAYTNGVDATHDAPSAFAPRADAALLDDWRSYFTAPALH
AWIDAALANNRDLRIAAGRLDEARAMYGVQRAERMPSIDASTGYERTRQYDPVLRESAVSGLYRAGVGISAYELDLFGRV
RNLSDAALAEYFATADAQRTVRIGVIAEVANAYVAERALNEQRALAERTLDARERIAALTQRRYAAGTSDAIELRSAEML
VAAARASHAALQREHAQAVRALQLLAGDFARDVRDDDTALDALTIAPVAPGAPSDLLERRPDIRQAESRLKAAHAQIGAA
RAAFFPRIALTTDYGSVSDAFSSLFAAGTSVWTFAPRITLPIFAGGRNRANLDVANARRHIAVAEYEKTVQVAFREVADA
FTARDWIERQLAAQRDVHAADDARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLRQLRLANAIALYRALGGGWTP
GTAADDA

Sequences:

>Translated_487_residues
MRHRSDSRRTARRAPFALAAALVLAGCSLAPRYERPAAPVPSAYTNGVDATHDAPSAFAPRADAALLDDWRSYFTAPALH
AWIDAALANNRDLRIAAGRLDEARAMYGVQRAERMPSIDASTGYERTRQYDPVLRESAVSGLYRAGVGISAYELDLFGRV
RNLSDAALAEYFATADAQRTVRIGVIAEVANAYVAERALNEQRALAERTLDARERIAALTQRRYAAGTSDAIELRSAEML
VAAARASHAALQREHAQAVRALQLLAGDFARDVRDDDTALDALTIAPVAPGAPSDLLERRPDIRQAESRLKAAHAQIGAA
RAAFFPRIALTTDYGSVSDAFSSLFAAGTSVWTFAPRITLPIFAGGRNRANLDVANARRHIAVAEYEKTVQVAFREVADA
FTARDWIERQLAAQRDVHAADDARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLRQLRLANAIALYRALGGGWTP
GTAADDA
>Mature_487_residues
MRHRSDSRRTARRAPFALAAALVLAGCSLAPRYERPAAPVPSAYTNGVDATHDAPSAFAPRADAALLDDWRSYFTAPALH
AWIDAALANNRDLRIAAGRLDEARAMYGVQRAERMPSIDASTGYERTRQYDPVLRESAVSGLYRAGVGISAYELDLFGRV
RNLSDAALAEYFATADAQRTVRIGVIAEVANAYVAERALNEQRALAERTLDARERIAALTQRRYAAGTSDAIELRSAEML
VAAARASHAALQREHAQAVRALQLLAGDFARDVRDDDTALDALTIAPVAPGAPSDLLERRPDIRQAESRLKAAHAQIGAA
RAAFFPRIALTTDYGSVSDAFSSLFAAGTSVWTFAPRITLPIFAGGRNRANLDVANARRHIAVAEYEKTVQVAFREVADA
FTARDWIERQLAAQRDVHAADDARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLRQLRLANAIALYRALGGGWTP
GTAADDA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=468, Percent_Identity=38.4615384615385, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI2367348, Length=485, Percent_Identity=24.7422680412371, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52913; Mature: 52913

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHRSDSRRTARRAPFALAAALVLAGCSLAPRYERPAAPVPSAYTNGVDATHDAPSAFAP
CCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
RADAALLDDWRSYFTAPALHAWIDAALANNRDLRIAAGRLDEARAMYGVQRAERMPSIDA
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCC
STGYERTRQYDPVLRESAVSGLYRAGVGISAYELDLFGRVRNLSDAALAEYFATADAQRT
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
VRIGVIAEVANAYVAERALNEQRALAERTLDARERIAALTQRRYAAGTSDAIELRSAEML
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAARASHAALQREHAQAVRALQLLAGDFARDVRDDDTALDALTIAPVAPGAPSDLLERR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCCCCCCHHHHHCC
PDIRQAESRLKAAHAQIGAARAAFFPRIALTTDYGSVSDAFSSLFAAGTSVWTFAPRITL
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCHHEEECCCEEE
PIFAGGRNRANLDVANARRHIAVAEYEKTVQVAFREVADAFTARDWIERQLAAQRDVHAA
EEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLRQLRLANAIALYRALGGGWTP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTAADDA
CCCCCCC
>Mature Secondary Structure
MRHRSDSRRTARRAPFALAAALVLAGCSLAPRYERPAAPVPSAYTNGVDATHDAPSAFAP
CCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
RADAALLDDWRSYFTAPALHAWIDAALANNRDLRIAAGRLDEARAMYGVQRAERMPSIDA
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCC
STGYERTRQYDPVLRESAVSGLYRAGVGISAYELDLFGRVRNLSDAALAEYFATADAQRT
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
VRIGVIAEVANAYVAERALNEQRALAERTLDARERIAALTQRRYAAGTSDAIELRSAEML
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAARASHAALQREHAQAVRALQLLAGDFARDVRDDDTALDALTIAPVAPGAPSDLLERR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCCCCCCHHHHHCC
PDIRQAESRLKAAHAQIGAARAAFFPRIALTTDYGSVSDAFSSLFAAGTSVWTFAPRITL
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCHHEEECCCEEE
PIFAGGRNRANLDVANARRHIAVAEYEKTVQVAFREVADAFTARDWIERQLAAQRDVHAA
EEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLRQLRLANAIALYRALGGGWTP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTAADDA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]