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The map label for this gene is ywbI [H]

Identifier: 161524725

GI number: 161524725

Start: 1703613

End: 1704506

Strand: Reverse

Name: ywbI [H]

Synonym: Bmul_1552

Alternate gene names: 161524725

Gene position: 1704506-1703613 (Counterclockwise)

Preceding gene: 161524726

Following gene: 161524723

Centisome position: 49.43

GC content: 68.79

Gene sequence:

>894_bases
ATGGTGAACCCGCTTCATTTCGATCTGCAGTCGTTGCGCGTATTCGCGCTCGTCGCCGAGCACGGCAGTCTCACGAAGGC
CGCCGAGCACGGGCAGCTCACGCTGTCGGCCGTCAGCAAACGCATCGCGGAACTCGAGAGCGTGACGGGCAGCGCGCTGT
TCGTCCGGCATGCGCGCGGCGTCGAGCTGACGCCCGCGGGCCGTGCGCTGCTCGATCATGCGGCGAAGATTCTCGAACAG
GTGAACCGGATGGCGCACGAGATGAGCGACTACGCCGCCGGCGTGCGCGGCCACATCCATGTGTGGACGAACACGTCGGC
CATCGTCCAGTTCCTGCCGGCCGATCTCGCGGCGTTTCTGACCGACAACCCCGGCATCCGGATCAGCCTCGCGGAGCGGC
TCAGCCACGAAATCGTCGATGCGCTCGCGGCCGGTCAGGCCGACCTCGGCGTGTTCGCGGACAACGTGCCGGCGCCCGGC
ATCGAGCGCCGCGTGTATCGACGCGACGAACTCGTGCTGCTGGTGCCGCGCGGCCATCGCTTCGCGACGCGCGAACGGAT
CCGCTTCGCCGAGACGCTCGACGAGGACTACGTCGGGCTCAGCGACGGCAGCTCGCTGCTCGCGCGAATGACCGATGCGG
CGTTCGCCGCCGAGCGGTCGCTGAAGCTGCGGATCCAGGTATCGAATTTCGACGGTGTGAGCCGGATGATCGAGGCCGGG
CTCGGGATCGGCGTGCTGCCGCGCGACGCCGTGACGGGCGAGCGCGCGGCACGGCTCGGCGTCGTGACGCTCGAGGATGC
GTGGGCGACGCGCACGCTGTGGGTCGGCGTGAAGGCCGGTGCGGCGCTGACCTCCGACATCGCGAAGCTTTTCGACTTCA
TGTCGGCACGCTGA

Upstream 100 bases:

>100_bases
CGCATGCGTGCGCGTATGATCGGCGTCAGGCCGACGCGCGCCCGCGCATACGCTACCGGCGCGGGCGCGGCTTTCTCCTC
AGGCTTTCCAGGACCTGCCC

Downstream 100 bases:

>100_bases
GGCGCCGACACGCAAACGGCCGGGCGGCGCGCGACGCGCGACGCGCCCGATTCGTCACACCGGATTGATCTCGTCCTGGC
TACGGCGCGTGGTCAGCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKILEQ
VNRMAHEMSDYAAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIRISLAERLSHEIVDALAAGQADLGVFADNVPAPG
IERRVYRRDELVLLVPRGHRFATRERIRFAETLDEDYVGLSDGSSLLARMTDAAFAAERSLKLRIQVSNFDGVSRMIEAG
LGIGVLPRDAVTGERAARLGVVTLEDAWATRTLWVGVKAGAALTSDIAKLFDFMSAR

Sequences:

>Translated_297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKILEQ
VNRMAHEMSDYAAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIRISLAERLSHEIVDALAAGQADLGVFADNVPAPG
IERRVYRRDELVLLVPRGHRFATRERIRFAETLDEDYVGLSDGSSLLARMTDAAFAAERSLKLRIQVSNFDGVSRMIEAG
LGIGVLPRDAVTGERAARLGVVTLEDAWATRTLWVGVKAGAALTSDIAKLFDFMSAR
>Mature_297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKILEQ
VNRMAHEMSDYAAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIRISLAERLSHEIVDALAAGQADLGVFADNVPAPG
IERRVYRRDELVLLVPRGHRFATRERIRFAETLDEDYVGLSDGSSLLARMTDAAFAAERSLKLRIQVSNFDGVSRMIEAG
LGIGVLPRDAVTGERAARLGVVTLEDAWATRTLWVGVKAGAALTSDIAKLFDFMSAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=25.3472222222222, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=24.9146757679181, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=190, Percent_Identity=29.4736842105263, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=28.8065843621399, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787806, Length=281, Percent_Identity=25.6227758007117, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=24.8, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=26.1044176706827, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786713, Length=137, Percent_Identity=35.036496350365, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=199, Percent_Identity=26.1306532663317, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=243, Percent_Identity=23.0452674897119, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082132, Length=258, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=154, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=297, Percent_Identity=24.5791245791246, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=26.4462809917355, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=296, Percent_Identity=21.6216216216216, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=275, Percent_Identity=25.4545454545455, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32192; Mature: 32192

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCC
VELTPAGRALLDHAAKILEQVNRMAHEMSDYAAGVRGHIHVWTNTSAIVQFLPADLAAFL
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TDNPGIRISLAERLSHEIVDALAAGQADLGVFADNVPAPGIERRVYRRDELVLLVPRGHR
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHCCCCEEEEEECCCC
FATRERIRFAETLDEDYVGLSDGSSLLARMTDAAFAAERSLKLRIQVSNFDGVSRMIEAG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCC
LGIGVLPRDAVTGERAARLGVVTLEDAWATRTLWVGVKAGAALTSDIAKLFDFMSAR
CCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCC
VELTPAGRALLDHAAKILEQVNRMAHEMSDYAAGVRGHIHVWTNTSAIVQFLPADLAAFL
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TDNPGIRISLAERLSHEIVDALAAGQADLGVFADNVPAPGIERRVYRRDELVLLVPRGHR
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHCCCCEEEEEECCCC
FATRERIRFAETLDEDYVGLSDGSSLLARMTDAAFAAERSLKLRIQVSNFDGVSRMIEAG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCC
LGIGVLPRDAVTGERAARLGVVTLEDAWATRTLWVGVKAGAALTSDIAKLFDFMSAR
CCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]