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The map label for this gene is nolF [H]

Identifier: 161524693

GI number: 161524693

Start: 1676190

End: 1677302

Strand: Reverse

Name: nolF [H]

Synonym: Bmul_1520

Alternate gene names: 161524693

Gene position: 1677302-1676190 (Counterclockwise)

Preceding gene: 161524695

Following gene: 161524692

Centisome position: 48.64

GC content: 72.33

Gene sequence:

>1113_bases
GTGAATCGCTCCGGTTCCCGTGCCGCGCTGCTGATCGGCGCCGCACTCGTTCTCGCCGCCTGTCATCCGAAGGAATCCGC
GCCGCCCGCGCCGCGCCCGGTCGTCGCGCTGCCCGCGCGCACGGATGCCGCCGCGTCCGCCTTCGCGCTGCCCGGCGAAA
TCCAGCCGCGCTACGCGACGCCGCTGTCGTTCCGCATCGCGGGCAAGATCGTCGAGCGCAAGGTGCGGCTCGGCGATACG
GTGAAGGCCGGTCAGGTCGTCGCACTGCTCGATCCGTCCGACGTCGAGAAAAACGCGGCGAGCGCGCAGGCGCAGCTCGA
CGCCGCCTCGCACCGGCTCGCATTCGCCAAACAGCAGCTCGACCGCGACCGCGCGCAGGCGCGCGAAAACCTGATCGCGA
CCGCGCAGCTCGAGCAGACCGAGGACAGCTACACGGCCGCGCTCGCGCAGCGCGATCAGGCGCAGCAGCAGCTCGCGCTC
GCGAAGAATCAGCTGCGCTACGCGACGCTCGTCGCCGATCATGCGGGCTACATCACCGCCGAGCAGGCCGACACCGGGCA
GAACGTGTCGGCCGGCCAGCCCGTCTACCAGCTCGCATGGGCCGGCGACGTCGACGTAGTCGTCGACGTGCCCGAAACGG
CGCTCGCGGGGCTGTCGCCCGGCCATCCGGCGACCGTCACGCTGCCGTCGCTGCCCGGCCGCACGTTCGCGGCGAAGGTG
CGCGAGATCGCGCCGGCCGCCGATCCGCAAAGCCGGACGTATCGGATGCGGCTCACGCTCGCCGCGCCGGACGCATCGGC
GATCCGTCTCGGGATGACCGCAAACGTCGCGTTCGACGGCGCGCCGCGCGCCGCCGACGCGCAACCGGTCACGCTGCCCG
CGACCGCGCTGTTCCACGACGGCCCGCATCCGGCCGTCTGGATCGTGCGCGCGAAGGACGACACGCTCGAGCTGCGCCGC
GTCGAGGTCGCGCGCTTCAACGAACGCACCGTCACCGTGTCGCACGGCGTGCAGCCGGGCGAGCGCGTCGTGCTGCAGGG
CGTGCATACGGTCAGCGCCGGCGAGAAGGTCCGTGCGATCGCGCCGCTGCATCCCGAGGATTTCGCGTCATGA

Upstream 100 bases:

>100_bases
TATCCGATCGTGCTGTCCGGGCGTCGCGCTCGCGCGGCCCCGTCGGGGCAGGCGCCGCCGCGCTTGCCGACCTCTTTCGC
TCTCGTCCCTTGGGGGTTTC

Downstream 100 bases:

>100_bases
GCGCGGCCCGCGAAGAAGGACGCTTCAACCTGTCCGCGTGGGCGCTGCGCCATCAGGCGCTCGTCGTCTATCTGATCGTG
CTCGCGACGGTCGCGGGCAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARTDAAASAFALPGEIQPRYATPLSFRIAGKIVERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQLDAASHRLAFAKQQLDRDRAQARENLIATAQLEQTEDSYTAALAQRDQAQQQLAL
AKNQLRYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWAGDVDVVVDVPETALAGLSPGHPATVTLPSLPGRTFAAKV
REIAPAADPQSRTYRMRLTLAAPDASAIRLGMTANVAFDGAPRAADAQPVTLPATALFHDGPHPAVWIVRAKDDTLELRR
VEVARFNERTVTVSHGVQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS

Sequences:

>Translated_370_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARTDAAASAFALPGEIQPRYATPLSFRIAGKIVERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQLDAASHRLAFAKQQLDRDRAQARENLIATAQLEQTEDSYTAALAQRDQAQQQLAL
AKNQLRYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWAGDVDVVVDVPETALAGLSPGHPATVTLPSLPGRTFAAKV
REIAPAADPQSRTYRMRLTLAAPDASAIRLGMTANVAFDGAPRAADAQPVTLPATALFHDGPHPAVWIVRAKDDTLELRR
VEVARFNERTVTVSHGVQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS
>Mature_370_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARTDAAASAFALPGEIQPRYATPLSFRIAGKIVERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQLDAASHRLAFAKQQLDRDRAQARENLIATAQLEQTEDSYTAALAQRDQAQQQLAL
AKNQLRYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWAGDVDVVVDVPETALAGLSPGHPATVTLPSLPGRTFAAKV
REIAPAADPQSRTYRMRLTLAAPDASAIRLGMTANVAFDGAPRAADAQPVTLPATALFHDGPHPAVWIVRAKDDTLELRR
VEVARFNERTVTVSHGVQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=386, Percent_Identity=23.5751295336788, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=24.5454545454545, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081951, Length=187, Percent_Identity=28.8770053475936, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39113; Mature: 39113

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARTDAAASAFALPGEIQPRYAT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHEECCCCCCCCCCC
PLSFRIAGKIVERKVRLGDTVKAGQVVALLDPSDVEKNAASAQAQLDAASHRLAFAKQQL
CEEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRDRAQARENLIATAQLEQTEDSYTAALAQRDQAQQQLALAKNQLRYATLVADHAGYITA
HHHHHHHHHCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWAGDVDVVVDVPETALAGLSPGHPATVTLPSLPGRTFAAKV
CCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCCCCEEEECCCCCCHHHHHH
REIAPAADPQSRTYRMRLTLAAPDASAIRLGMTANVAFDGAPRAADAQPVTLPATALFHD
HHHCCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCEEECHHEEECC
GPHPAVWIVRAKDDTLELRRVEVARFNERTVTVSHGVQPGERVVLQGVHTVSAGEKVRAI
CCCCEEEEEEECCCCEEEEEEEEEECCCEEEEEECCCCCCCCEEEEECEEECCCCCEEEE
APLHPEDFAS
CCCCCHHCCC
>Mature Secondary Structure
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARTDAAASAFALPGEIQPRYAT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHEECCCCCCCCCCC
PLSFRIAGKIVERKVRLGDTVKAGQVVALLDPSDVEKNAASAQAQLDAASHRLAFAKQQL
CEEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRDRAQARENLIATAQLEQTEDSYTAALAQRDQAQQQLALAKNQLRYATLVADHAGYITA
HHHHHHHHHCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWAGDVDVVVDVPETALAGLSPGHPATVTLPSLPGRTFAAKV
CCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCCCCEEEECCCCCCHHHHHH
REIAPAADPQSRTYRMRLTLAAPDASAIRLGMTANVAFDGAPRAADAQPVTLPATALFHD
HHHCCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCEEECHHEEECC
GPHPAVWIVRAKDDTLELRRVEVARFNERTVTVSHGVQPGERVVLQGVHTVSAGEKVRAI
CCCCEEEEEEECCCCEEEEEEEEEECCCEEEEEECCCCCCCCEEEEECEEECCCCCEEEE
APLHPEDFAS
CCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]