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The map label for this gene is tesA [H]

Identifier: 161524528

GI number: 161524528

Start: 1492786

End: 1493340

Strand: Reverse

Name: tesA [H]

Synonym: Bmul_1355

Alternate gene names: 161524528

Gene position: 1493340-1492786 (Counterclockwise)

Preceding gene: 161524530

Following gene: 161524520

Centisome position: 43.3

GC content: 68.47

Gene sequence:

>555_bases
GTGATCGCCGTACTCGGCGACAGCCTCTCGGCCGAATACGGGCTGCCGCGCGACACCGGCTGGGTCGCGCTGCTGCGGCA
ACGGCTCGCGACGGAGCGAATCGATTATAGCGTCGCGAATGCGAGCGTCAGCGGCGACACCACGAGCGGCGGCCGCGCAC
GGCTGCCGGCCGTGCTGCAGCGGCTCAAGCCGTCGATCGTCGTCGTCGAGCTCGGCGCGAACGACGCACTGCGCGGCGTG
CCGCTCGCGACGACCGAGCAGAATCTGCGCGACATCGTCGCCGATGCGCGGCGCGCGCACGCCAAAGTCGTGCTGGTCGG
CATGTACGTGCCGTCGAATTACGGCCCCGACTACACGCAGAAGTTCCATGCGGTCTATACGCGACTGGCGAAGGAACTCG
ACGTGCCGCTCGTGCCGTTCCTGCTCGCCGGCATCGAGAACAAGCCCGACATGTTCCAGTCCGACCAGATCCATCCGACG
CAGCAGGCGCAGCGCGTGCTGCTCGACAACGTGTGGCCGACGCTGAAGCCGCTGCTCGGCAAGCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
GAAAAGACGCGCGGCGCTCGTCGCGCTGCTGGGCGGCACGCTCGCCGCGACGGGATTCGCTCGCGCCGCCGCGGCACCGG
CCGCGACCGCGGGACAGCCG

Downstream 100 bases:

>100_bases
AGCGCCCGCCCCAATGAAAAAATCCCTGCCGGCGCAATGCCGGACAGGGATCGGAATGCGGATCGCGCCGCGCTGCCGCC
GGGCGGCGCACGACGCGCAT

Product: GDSL family lipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MIAVLGDSLSAEYGLPRDTGWVALLRQRLATERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGANDALRGV
PLATTEQNLRDIVADARRAHAKVVLVGMYVPSNYGPDYTQKFHAVYTRLAKELDVPLVPFLLAGIENKPDMFQSDQIHPT
QQAQRVLLDNVWPTLKPLLGKPRG

Sequences:

>Translated_184_residues
MIAVLGDSLSAEYGLPRDTGWVALLRQRLATERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGANDALRGV
PLATTEQNLRDIVADARRAHAKVVLVGMYVPSNYGPDYTQKFHAVYTRLAKELDVPLVPFLLAGIENKPDMFQSDQIHPT
QQAQRVLLDNVWPTLKPLLGKPRG
>Mature_184_residues
MIAVLGDSLSAEYGLPRDTGWVALLRQRLATERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGANDALRGV
PLATTEQNLRDIVADARRAHAKVVLVGMYVPSNYGPDYTQKFHAVYTRLAKELDVPLVPFLLAGIENKPDMFQSDQIHPT
QQAQRVLLDNVWPTLKPLLGKPRG

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=179, Percent_Identity=44.6927374301676, Blast_Score=153, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 20121; Mature: 20121

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVLGDSLSAEYGLPRDTGWVALLRQRLATERIDYSVANASVSGDTTSGGRARLPAVLQ
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RLKPSIVVVELGANDALRGVPLATTEQNLRDIVADARRAHAKVVLVGMYVPSNYGPDYTQ
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHH
KFHAVYTRLAKELDVPLVPFLLAGIENKPDMFQSDQIHPTQQAQRVLLDNVWPTLKPLLG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
KPRG
CCCC
>Mature Secondary Structure
MIAVLGDSLSAEYGLPRDTGWVALLRQRLATERIDYSVANASVSGDTTSGGRARLPAVLQ
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RLKPSIVVVELGANDALRGVPLATTEQNLRDIVADARRAHAKVVLVGMYVPSNYGPDYTQ
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHH
KFHAVYTRLAKELDVPLVPFLLAGIENKPDMFQSDQIHPTQQAQRVLLDNVWPTLKPLLG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
KPRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]