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The map label for this gene is def1 [H]

Identifier: 161524426

GI number: 161524426

Start: 1380320

End: 1380853

Strand: Direct

Name: def1 [H]

Synonym: Bmul_1253

Alternate gene names: 161524426

Gene position: 1380320-1380853 (Clockwise)

Preceding gene: 161524425

Following gene: 161524430

Centisome position: 40.03

GC content: 64.23

Gene sequence:

>534_bases
ATGATTCGCGAAATCCTGAAGATGGGCGATCCGCGCCTGCTCGAGGTCGCGCGGCCGGTCGACCGGTTCGACACGCCGGA
ATTGCACGAGATCGTCGCGGACATGTTCGAGACGATGCATCACGCGAACGGCGCCGGGCTTGCGGCGCCGCAGATCGGCA
TCGGGCTGCAGATCATCATCTTCGGCTTCGGCAGCAACGACCGCTATCCGGACGCGCCGCCCGTCCCCGAGACGGTGCTG
ATCAATCCGAAGCTCGAATACATGCCGCCGGACATGGAAGAGGGCTGGGAAGGCTGTCTGTCGGTGCCGGGGATGCGCGG
TGTCGTGAGCCGCTATGCGAAGGTGCGCTACAGCGGCTTCGACCAGTTCGGCGCGAAGATCGATCGCGTCGCCGAAGGCT
TCCATGCGCGCGTGGTCCAGCACGAATACGACCACCTGATCGGCAAGCTGTATCCGATGCGGATCACCGACTTCACGCGT
TTCGGCTTCACTGAAGTGCTGTTCCCGGGGCTCGATCCGGCCGCCGACGACTGA

Upstream 100 bases:

>100_bases
CGGGCGCCGAAGCCGGCAGCAAGCTCGCGAAGGCCGAAGCGCTCGGCATTCCGGTGCTCGACGAAGACGGGCTGCATCAA
CTCCTGGAGGGCAACACGCG

Downstream 100 bases:

>100_bases
CGGCCGATGCCGCAGGCGCGGCGACCTTGCGGCCGCGCGCGTCGTGATGAACGGAGAAAGAAAAAGCCCGCCGTCGATGG
CGGGCTTTTCCGTTGATGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILKMGDPRLLEVARPVDRFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQIIIFGFGSNDRYPDAPPVPETVL
INPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQFGAKIDRVAEGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPAADD

Sequences:

>Translated_177_residues
MIREILKMGDPRLLEVARPVDRFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQIIIFGFGSNDRYPDAPPVPETVL
INPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQFGAKIDRVAEGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPAADD
>Mature_177_residues
MIREILKMGDPRLLEVARPVDRFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQIIIFGFGSNDRYPDAPPVPETVL
INPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQFGAKIDRVAEGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPAADD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=32, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=40.2684563758389, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=183, Percent_Identity=33.879781420765, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=36.5384615384615, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19861; Mature: 19861

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDPRLLEVARPVDRFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQIII
CHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFGSNDRYPDAPPVPETVLINPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGF
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
DQFGAKIDRVAEGFHARVVQHEYDHLIGKLYPMRITDFTRFGFTEVLFPGLDPAADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDPRLLEVARPVDRFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQIII
CHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFGSNDRYPDAPPVPETVLINPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGF
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
DQFGAKIDRVAEGFHARVVQHEYDHLIGKLYPMRITDFTRFGFTEVLFPGLDPAADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]