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The map label for this gene is ligA [H]

Identifier: 161524425

GI number: 161524425

Start: 1378248

End: 1380323

Strand: Direct

Name: ligA [H]

Synonym: Bmul_1252

Alternate gene names: 161524425

Gene position: 1378248-1380323 (Clockwise)

Preceding gene: 161524424

Following gene: 161524426

Centisome position: 39.97

GC content: 68.45

Gene sequence:

>2076_bases
ATGGCCCGAACCCAAGCCGAACCGCCAGCCAGTCAACCCGATGTGCGCGCCGCCTGGCTGCGCGACCAGCTCGAACGCGC
GAACTACGCGTACTACGTGCTCGATCAGCCCGATCTGCCCGATGCGGAATACGATCGGCTGTTCCGCGAGCTGCAGCAGC
TCGAATCCGATCATCCCGATCTCGTCACGCCCGATTCGCCGACGCAGCGCGTCGGCGGCGCCGTCGCGAGCGGCTTCACG
CCGGTCGTTCACGATGCGCCGATGCTGTCGCTGAACAACGGGTTCGCCGACGACGACATCGTCGCGTTCGACAAGCGCGT
CGCCGACGCGCTCGGCAAGACGACCGATCTCGCCGGCTCGGTGACGGATCCCGTCGAATACGCGTGCGAGCTGAAGTTCG
ACGGGCTCGCGATTTCGCTGCGCTACGAGCACGGCGTGTTCGTGCAGGCGTCGACGCGCGGCGATGGCACGACCGGCGAG
GACGTGACCGAAAACGTCCGCACGATCCGCTCGATTCCGCTGAAGCTGAAGGGCGACCGCGTGCCGGCCGTGCTCGACGT
GCGCGGCGAGGTGCTGATGTTCAAGCGCGATTTTGCGCGCCTGAACGAACGCCAGCGCGCGGCCGAGCAGCGCGAGTTCG
CGAACCCGCGCAACGCGGCGGCCGGCAGCCTGCGCCAGCTCGATCCGAAGATCACCGCGCAGCGTCCGCTCTCGTTCTTC
GCGTACGGCATCGGCGTGCTCGACGGGATGCCGATGCCCGATACGCACAGCGCGCTGCTCGACTGGTACGAGGCGCTCGG
GCTGCCGGTGAACCGCGAGCGCGCGGTCGTGTACGGCGCGCAGGGGCTGCTCGACTTCTTCCGCAAGGTCGGCGAGAAGC
GCGAGTCGCTGCCGTACGACATCGACGGCGTCGTGTACAAGGTGAACCGCCGCGACGAGCAGGATCGCCTCGGTTTCGTG
TCGCGCGCGCCGCGCTTCGCGCTCGCACACAAATTCCCCGCGCAGGAGGCGCTGACGAAGCTGGTCGCCATCGGCGTGCA
GGTCGGCCGCACCGGCGCGATCACGCCGGTCGCGCGGCTCGAGCCCGTGTTCGTCGGCGGCGCGACCGTCACGAACGCGA
CGCTGCACAACGAGGACGAGGTGCGCCGTAAGGACATCCGGATCGGCGATACGGTGATCGTGCGGCGCGCGGGCGACGTG
ATTCCGGAGGTGGTGGGCGCCGTGCTCGACCGGCGTCCGGCCGACGCGGCCGAATTCGTGATGCCGACCGAATGCCCGGT
ATGCGGCTCGAAGATCGAGCGGCTGCCCGACGAGGCGATCGCGCGCTGCACGGGCGGGCTGTTTTGCCCCGCGCAGCGCA
AGCAGGCGCTGTGGCATTTCGCGCAGCGGCGCGCGCTCGACATCGACGGGCTCGGCGAGAAGATCATCGACCAGCTCGTG
GAGCTGAACCTCGTACGCACGCCGGCCGATCTGTTCAACCTCGGCTTCGCGACGCTCGCGGAGCTCGACCGCTTCGCGGA
GAAATCGGCGCAGAATCTGCTCGACTCGCTCGAGAAGGCGAAGCACACGACGCTCGCGCGCTTCATTTACGCGCTCGGCA
TCCGCCATGTCGGCGAATCGACCGCGAAGGATCTCGCCAAGCATTTCGGCTCGCTCGACCCGATCATGAACGCGACGATC
GACGAGCTGCTCGAAGTGAACGACGTCGGGCCGATCGTCGCCGAATCGATCCACCAGTTCTTCGCGGAAGAGCACAACCG
GACCGTGATCGAGCAGCTGCGCGCGCCGGGCAAGGTCACCTGGCCGGAAGGGCCGCCCGCGCCGAAGGCGCCGCAGGGCG
TGCTCGCCGGCAAGACGGTCGTGCTGACCGGCACGCTGCCGAACCTCACGCGCGACGACGCGAAGGAGATGCTCGAGGCG
GCGGGCGCGAAGGTGGCCGGCTCGGTGTCGAAGAAGACCGATTACGTGATCGCGGGCGCCGAAGCCGGCAGCAAGCTCGC
GAAGGCCGAAGCGCTCGGCATTCCGGTGCTCGACGAAGACGGGCTGCATCAACTCCTGGAGGGCAACACGCGATGA

Upstream 100 bases:

>100_bases
GCGACGCAGCATCGCGCCGGCACGCAGGCCGCGCCCCCGGCACGGCGCCAAACTGAGATAATTCGGCATTCGATTTTTTC
CGCAAGAATCTGCCGCCAGC

Downstream 100 bases:

>100_bases
TTCGCGAAATCCTGAAGATGGGCGATCCGCGCCTGCTCGAGGTCGCGCGGCCGGTCGACCGGTTCGACACGCCGGAATTG
CACGAGATCGTCGCGGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARTQAEPPASQPDVRAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLESDHPDLVTPDSPTQRVGGAVASGFT
PVVHDAPMLSLNNGFADDDIVAFDKRVADALGKTTDLAGSVTDPVEYACELKFDGLAISLRYEHGVFVQASTRGDGTTGE
DVTENVRTIRSIPLKLKGDRVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDPKITAQRPLSFF
AYGIGVLDGMPMPDTHSALLDWYEALGLPVNRERAVVYGAQGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLVAIGVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGAVLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMNATI
DELLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDDAKEMLEA
AGAKVAGSVSKKTDYVIAGAEAGSKLAKAEALGIPVLDEDGLHQLLEGNTR

Sequences:

>Translated_691_residues
MARTQAEPPASQPDVRAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLESDHPDLVTPDSPTQRVGGAVASGFT
PVVHDAPMLSLNNGFADDDIVAFDKRVADALGKTTDLAGSVTDPVEYACELKFDGLAISLRYEHGVFVQASTRGDGTTGE
DVTENVRTIRSIPLKLKGDRVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDPKITAQRPLSFF
AYGIGVLDGMPMPDTHSALLDWYEALGLPVNRERAVVYGAQGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLVAIGVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGAVLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMNATI
DELLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDDAKEMLEA
AGAKVAGSVSKKTDYVIAGAEAGSKLAKAEALGIPVLDEDGLHQLLEGNTR
>Mature_690_residues
ARTQAEPPASQPDVRAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLESDHPDLVTPDSPTQRVGGAVASGFTP
VVHDAPMLSLNNGFADDDIVAFDKRVADALGKTTDLAGSVTDPVEYACELKFDGLAISLRYEHGVFVQASTRGDGTTGED
VTENVRTIRSIPLKLKGDRVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDPKITAQRPLSFFA
YGIGVLDGMPMPDTHSALLDWYEALGLPVNRERAVVYGAQGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQDRLGFVS
RAPRFALAHKFPAQEALTKLVAIGVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVI
PEVVGAVLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVE
LNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMNATID
ELLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDDAKEMLEAA
GAKVAGSVSKKTDYVIAGAEAGSKLAKAEALGIPVLDEDGLHQLLEGNTR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=46.1538461538462, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=58.1464872944694, Blast_Score=764, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=41.1111111111111, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI62472391, Length=92, Percent_Identity=42.3913043478261, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17737765, Length=92, Percent_Identity=42.3913043478261, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75501; Mature: 75369

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTQAEPPASQPDVRAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLESDHPD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGAVASGFTPVVHDAPMLSLNNGFADDDIVAFDKRVADALGKTTDLAGS
CCCCCCCHHHHCCHHHCCCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
VTDPVEYACELKFDGLAISLRYEHGVFVQASTRGDGTTGEDVTENVRTIRSIPLKLKGDR
CCCHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDPKITAQRPLSFF
CCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCHHHCCHHHH
AYGIGVLDGMPMPDTHSALLDWYEALGLPVNRERAVVYGAQGLLDFFRKVGEKRESLPYD
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLVAIGVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGAVLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMNATIDELLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDDAKEMLEAAGAKVAGSVSKKTDYVIAGA
CCCCCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLAKAEALGIPVLDEDGLHQLLEGNTR
CCCCHHHHHHHCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ARTQAEPPASQPDVRAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLESDHPD
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGAVASGFTPVVHDAPMLSLNNGFADDDIVAFDKRVADALGKTTDLAGS
CCCCCCCHHHHCCHHHCCCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
VTDPVEYACELKFDGLAISLRYEHGVFVQASTRGDGTTGEDVTENVRTIRSIPLKLKGDR
CCCHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDPKITAQRPLSFF
CCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCHHHCCHHHH
AYGIGVLDGMPMPDTHSALLDWYEALGLPVNRERAVVYGAQGLLDFFRKVGEKRESLPYD
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLVAIGVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGAVLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMNATIDELLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDDAKEMLEAAGAKVAGSVSKKTDYVIAGA
CCCCCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLAKAEALGIPVLDEDGLHQLLEGNTR
CCCCHHHHHHHCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA