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The map label for this gene is prfB [H]

Identifier: 161524328

GI number: 161524328

Start: 1269612

End: 1270358

Strand: Reverse

Name: prfB [H]

Synonym: Bmul_1153

Alternate gene names: 161524328

Gene position: 1270358-1269612 (Counterclockwise)

Preceding gene: 161524329

Following gene: 161524327

Centisome position: 36.84

GC content: 64.26

Gene sequence:

>747_bases
ATGTTCTCGAACCCGGCCGACCCGAACAACTGCTTCATCGACATCCAGGCCGGCGCCGGCGGCACCGAGGCCTGCGACTG
GGCGTCGATGCTGCTGCGCCAGTACCTGCGCTACTGCGAGCGCAAGGGCTTCAAGGCCGAAGTGCTCGAAGAATCCGACG
GCGACGTCGCGGGCATCAAGAACGCGACGATCAAGGTGTCGGGCGAATACGCATACGGCTACCTGCGCACCGAAACCGGC
ATCCACCGGCTCGTGCGCAAGTCGCCGTTCGATTCGTCGGGCGGCCGTCACACGTCGTTCTCGTCGGTGTTCGTCTATCC
GGAAATCGACGACTCGATCGAAGTCGAGATCAACCCGGCCGACCTGCGGATCGACACGTACCGCGCATCGGGCGCGGGCG
GCCAGCACATCAACAAGACCGACTCGGCGGTGCGGATCACGCACATGCCGACCGGCATCGTCGTGCAGTGCCAGAACGAC
CGCTCGCAGCACCGCAACCGCGCGGAAGCGATGGCGATGCTGAAGTCGCGCCTGTACGAAGCCGAGCTGCGCAAGCGCCA
GGCCGAGCAGGACAAGCTCGAATCGAGCAAGACCGACGTGGGCTGGGGTCACCAGATCCGCTCGTACGTGCTCGACCAGA
GCCGCGTGAAGGATCTGCGTACGAACGTCGAAATGAGCAACACGAAGGCGGTGCTCGACGGCGATCTCGACGACTTCATC
AGCGCGAGCCTCAAACAGGGCGTGTAA

Upstream 100 bases:

>100_bases
CGAGCTTGCACGCGAGGAAGGCGACGAGGACACGCTCATCGCCTCCGAAGAAGACGCCGCGAAGCTCGAAGCGCGCGTCG
CCGACATCGAATTCCGCCGG

Downstream 100 bases:

>100_bases
GCGCCGCGGCGCGGCCCGGCCGCGCCGCCCTTCCCGTTTGCGTTGCCGCACCCATTCCGAATTCCGACCATCATGACCGA
ACCGACCCAAACGCAGCCCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATIKVSGEYAYGYLRTETG
IHRLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQND
RSQHRNRAEAMAMLKSRLYEAELRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTKAVLDGDLDDFI
SASLKQGV

Sequences:

>Translated_248_residues
MFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATIKVSGEYAYGYLRTETG
IHRLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQND
RSQHRNRAEAMAMLKSRLYEAELRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTKAVLDGDLDDFI
SASLKQGV
>Mature_248_residues
MFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATIKVSGEYAYGYLRTETG
IHRLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQND
RSQHRNRAEAMAMLKSRLYEAELRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTKAVLDGDLDDFI
SASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=249, Percent_Identity=39.7590361445783, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=38.4, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=41.6107382550336, Blast_Score=122, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=248, Percent_Identity=69.758064516129, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=43.9024390243902, Blast_Score=213, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=238, Percent_Identity=34.8739495798319, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=249, Percent_Identity=34.5381526104418, Blast_Score=148, Evalue=6e-37,
Organism=Drosophila melanogaster, GI19921226, Length=213, Percent_Identity=42.7230046948357, Blast_Score=161, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 27662; Mature: 27662

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIK
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECC
NATIKVSGEYAYGYLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEINPA
CCEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCC
DLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSRLYE
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AELRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTKAVLDGDLDDFI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEECCCHHHHH
SASLKQGV
HHHHHHCC
>Mature Secondary Structure
MFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIK
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECC
NATIKVSGEYAYGYLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEINPA
CCEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCC
DLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSRLYE
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AELRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTKAVLDGDLDDFI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEECCCHHHHH
SASLKQGV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA