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The map label for this gene is lysS [H]

Identifier: 161524327

GI number: 161524327

Start: 1268013

End: 1269539

Strand: Reverse

Name: lysS [H]

Synonym: Bmul_1152

Alternate gene names: 161524327

Gene position: 1269539-1268013 (Counterclockwise)

Preceding gene: 161524328

Following gene: 161524326

Centisome position: 36.81

GC content: 65.03

Gene sequence:

>1527_bases
ATGACCGAACCGACCCAAACGCAGCCCGCCGTCGCGGCGGACGAAAACCAGATCATCGCCGAACGCCGCGACAAGCTCCG
CGCCTTGCGCGAGCAAGGCGTCGCCTATCCGAACGACTTCCGCCCGACGCACCGCGCAGCGGAGCTGCAGGCGAAATTCG
CCGAGTCGGACAAGGAAGCGCTCGAAGCGAACCCGTTCGAGGTCGCGGTGGCCGGCCGGATGATGCTCAAGCGCGTGATG
GGCAAGGCGAGCTTCGCGACCGTGCAGGACGGTTCGGGCCAGATCCAGTTCTTCGTGACGCCGAACGACGTCGGCGCCGA
AACCTACGACGCATTCAAGAAGTGGGACCTCGGCGACATCGTCGCCGCGCGCGGCGTGCTGTTCCGCACCAACAAGGGCG
AGCTGTCGGTCAAGTGCACGGAGCTGCGGCTGCTGTCGAAGGCGCTGCGTCCGCTGCCCGACAAGTTCCACGGCCTGTCC
GACCAGGAAATGCGCTATCGGCAGCGCTACGTCGACCTGATCGTCACGCCGGAGACGCGCGACACGTTCCGTGCGCGCAC
GAAGACGATCGCGTCGATCCGCAAGTTCATGAGCGATGCCGAGTTCATGGAAGTCGAGACGCCGATGCTGCACCCGATTC
CGGGCGGCGCCGCGGCGAAGCCGTTCGTCACGCATCACAATGCGCTCGACATGCAGATGTTCCTGCGCATCGCGCCGGAG
CTGTACCTGAAGCGGCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAACCGCAATTTCCGGAACGAAGGCGTGTC
GCCGCGCCACAATCCGGAATTCACGATGATGGAGTTCTACGCGGCGTACACCGACTATCGCTGGCTGATGGACTTCACCG
AGCAGCTGATCCGCCAGGCCGCGATCGATGCGCTCGGCACCGCGACGATCCAGTACCAGGGCCGCGAGCTCGATCTCGCG
AAGCCGTTCCATCGGCTCACGATCACGCAGGCGATCCAGAAGTACGCGCCGCAGTACACCGACGGTCAGCTGTCCGACAA
CGCGTATCTGCGCAGCGAACTGAAGCGCCTCGGCGTCGACGTCACGCAGCCCGCGTTCCTGAACGCCGGCATCGGCGCGT
TGCAGCTCGCGCTGTTCGAGGAAACGGCCGAATCGCAGCTGTGGGAACCGACGTTCATCATCGACTACCCGGTCGAAGTG
TCGCCGCTCGCACGCGCATCGGACACGGTGCCGGGCATCACCGAGCGCTTCGAGCTGTTCATCACCGGTCGCGAGATCGC
GAACGGCTTCTCGGAGCTGAACGATCCGGAAGATCAGGCCGCACGCTTCAAGAAGCAGGTCGAGCAGAAGGACGCGGGCG
ACGAGGAAGCGATGTACTTCGACGCCGACTATATCCGCGCGCTCGAATACGGGATGCCGCCGACCGGCGGCTGCGGGATC
GGCATCGACCGTCTGGTCATGCTGCTGACCGACAGCCCGACGATCCGCGACGTGCTGCTGTTCCCGCACCTGCGTCGCGA
AGACTGA

Upstream 100 bases:

>100_bases
CAGCGCGAGCCTCAAACAGGGCGTGTAAGCGCCGCGGCGCGGCCCGGCCGCGCCGCCCTTCCCGTTTGCGTTGCCGCACC
CATTCCGAATTCCGACCATC

Downstream 100 bases:

>100_bases
CGATTCGCGCGCTTGCGTGCGCCGCTGCTGCGGCGCACGCGCGTCAGCTTTGTAACGGCGCGTAAATCCTGCCGGCCGAT
ACGCGTCGGCTGTCGATCCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTEPTQTQPAVAADENQIIAERRDKLRALREQGVAYPNDFRPTHRAAELQAKFAESDKEALEANPFEVAVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTELRLLSKALRPLPDKFHGLS
DQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMSDAEFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPQYTDGQLSDNAYLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPVEV
SPLARASDTVPGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMYFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED

Sequences:

>Translated_508_residues
MTEPTQTQPAVAADENQIIAERRDKLRALREQGVAYPNDFRPTHRAAELQAKFAESDKEALEANPFEVAVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTELRLLSKALRPLPDKFHGLS
DQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMSDAEFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPQYTDGQLSDNAYLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPVEV
SPLARASDTVPGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMYFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED
>Mature_507_residues
TEPTQTQPAVAADENQIIAERRDKLRALREQGVAYPNDFRPTHRAAELQAKFAESDKEALEANPFEVAVAGRMMLKRVMG
KASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTELRLLSKALRPLPDKFHGLSD
QEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMSDAEFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPEL
YLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLAK
PFHRLTITQAIQKYAPQYTDGQLSDNAYLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPVEVS
PLARASDTVPGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMYFDADYIRALEYGMPPTGGCGIG
IDRLVMLLTDSPTIRDVLLFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=510, Percent_Identity=39.4117647058824, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI194272210, Length=510, Percent_Identity=39.4117647058824, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI40789249, Length=327, Percent_Identity=22.9357798165138, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=58.498023715415, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1790571, Length=506, Percent_Identity=57.9051383399209, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=31.9018404907975, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788173, Length=250, Percent_Identity=25.2, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=521, Percent_Identity=40.4990403071017, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17535925, Length=521, Percent_Identity=40.4990403071017, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=41.8032786885246, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI32566633, Length=280, Percent_Identity=23.5714285714286, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=41.8111753371869, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=486, Percent_Identity=32.0987654320988, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6323011, Length=507, Percent_Identity=24.8520710059172, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=455, Percent_Identity=23.956043956044, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640849, Length=519, Percent_Identity=40.655105973025, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=519, Percent_Identity=40.655105973025, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24584738, Length=318, Percent_Identity=25.4716981132075, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57605; Mature: 57474

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTQTQPAVAADENQIIAERRDKLRALREQGVAYPNDFRPTHRAAELQAKFAESDKEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
LEANPFEVAVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDI
HCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVKCTELRLLSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTKTIASIRKFMSDAEFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPQYTDGQLSDNAYLRSELKRLGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VTQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPVEVSPLARASDTVPGITERFELF
CCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCCCCCCHHHEEEEE
ITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMYFDADYIRALEYGMPPTGGCGI
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHHHHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEPTQTQPAVAADENQIIAERRDKLRALREQGVAYPNDFRPTHRAAELQAKFAESDKEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
LEANPFEVAVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDI
HCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVKCTELRLLSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTKTIASIRKFMSDAEFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPQYTDGQLSDNAYLRSELKRLGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VTQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPVEVSPLARASDTVPGITERFELF
CCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCCCCCCHHHEEEEE
ITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMYFDADYIRALEYGMPPTGGCGI
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHHHHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA