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The map label for this gene is iscA [H]

Identifier: 161524321

GI number: 161524321

Start: 1263249

End: 1263572

Strand: Direct

Name: iscA [H]

Synonym: Bmul_1146

Alternate gene names: 161524321

Gene position: 1263249-1263572 (Clockwise)

Preceding gene: 161524320

Following gene: 161524322

Centisome position: 36.63

GC content: 61.73

Gene sequence:

>324_bases
ATGGCAATTACACTGACCGAAAAAGCAGCACAGCACGTCCAGAAATACCTGGTCCGTCGCGGCAAGGGCCTGGGCCTGCG
GCTCGGCGTTCGCACGACCGGGTGCTCGGGGCTCGCGTACAAGCTCGAGTATGTCGACGAGCTTGCCCCCGAGGATCAGG
TGTTCGAGAGCCATGGCGTGAAGGTCGTCGTCGACCCGAAAAGCCTCGCGTATATCGACGGCACCGAGCTCGACTTCGCG
CGCGAAGGCCTGAACGAAGGGTTCAAGTTCAACAACCCGAACGTGAAGGACGAGTGCGGCTGCGGCGAATCCTTCCGCGT
CTGA

Upstream 100 bases:

>100_bases
GCCACGACACGAAGCAGGACGATCAGGCAGCGGCCTGACGCATCGAGCGGCTTGTGAAGGGAATCGCGGCGTGCCGACGG
CGCGCCGCGGCAAGGACATC

Downstream 100 bases:

>100_bases
CGCGGATCTCCGCGCGACGCGGGCAAGAGGCGGCACGGGCCGCCTTTTTAATGGGCGGCGTTCGCCGCACGACGAACCGG
AATTGAACGCGACGATGGTC

Product: iron-sulfur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAITLTEKAAQHVQKYLVRRGKGLGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVVVDPKSLAYIDGTELDFA
REGLNEGFKFNNPNVKDECGCGESFRV

Sequences:

>Translated_107_residues
MAITLTEKAAQHVQKYLVRRGKGLGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVVVDPKSLAYIDGTELDFA
REGLNEGFKFNNPNVKDECGCGESFRV
>Mature_106_residues
AITLTEKAAQHVQKYLVRRGKGLGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVVVDPKSLAYIDGTELDFAR
EGLNEGFKFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=40.5660377358491, Blast_Score=94, Evalue=2e-20,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=43.3734939759036, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=71.9626168224299, Blast_Score=164, Evalue=9e-43,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=48.5714285714286, Blast_Score=112, Evalue=6e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=38.1818181818182, Blast_Score=72, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=39.622641509434, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.9622641509434, Blast_Score=61, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=45.7142857142857, Blast_Score=96, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=42.4528301886792, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11796; Mature: 11664

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITLTEKAAQHVQKYLVRRGKGLGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGV
CEEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
KVVVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AITLTEKAAQHVQKYLVRRGKGLGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGV
EEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
KVVVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]