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The map label for this gene is puuA [H]

Identifier: 161524282

GI number: 161524282

Start: 1210675

End: 1212012

Strand: Direct

Name: puuA [H]

Synonym: Bmul_1107

Alternate gene names: 161524282

Gene position: 1210675-1212012 (Clockwise)

Preceding gene: 161524281

Following gene: 161524283

Centisome position: 35.11

GC content: 64.28

Gene sequence:

>1338_bases
ATGCAACCCGAACTGACCGAATTCCTGCGCCAGCACCGCATCACCGAGGTCGAAGCGATCATCCCCGACATGGCGGGCAT
CGCGCGCGGGAAGATCATTCCGCGCAACAAATTCGAATCCGGCGAATCGATGCGGCTGCCGCAGGCCGTGATGGTGCAGA
CCGTCACCGGCGACTATCCGGAAGACGGCACGCTGACCGGCGTGACCGATCCCGACATGGTGTGCGTGCCCGATCCGTCG
ACGATCTGCCTGATCCCGTGGGCCGTCGATCCGACCGCGCAGGTGATTCACGACTGCGTGCACTTCGACGGCTCGCCGGT
CGAGATCTCGCCGCGCTACGTGCTCCGCCGCGTGCTCGAGCTGTACAAGCAGAAGGGGTGGCAGCCGGTCGTCGCGCCCG
AGCTCGAGTTTTATCTGGTCGACATGAATGCCGATCCCGACTTGCCGCTGCGTCCGCCGGTCGGCCGCACCGGTCGCGCG
GAGACGGGCCGGCAGTCGTATTCGATCGAGGCCGTCAACGAGTTCGATCCGCTGTTCGAAGACATCTATGAATACTGCGA
GATGCAGGGGCTCGACATCGAGACGCTGATCCATGAGGTCGGCGCCGCGCAGATGGAGATCAACTTCGTGCACGGCGACG
CGCTGTCGCTTGCCGATCAGGTGTTCCTGTTCAAGCGCACCGTGCGCGAAGCGGCGCTGCGCCACAACATGTATGCGACG
TTCATGGCCAAGCCGATGGAAGGCGAACCGGGCTCGGCGATGCACATCCATCAGAGCCTCGCGGATGCGCGCACCGGACG
CAACCTGTTCGCCGACGAAGGCGGCGCCGTGTCGCCGATGTTCCACAGCTATCTCGCGGGGCTGCAGAAATACACGCCCG
CGCTGATGCCGATCTTCGCGCCGTACATCAACTCGTATCGCCGGTTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAA
TGGGGCTACGACAACCGCACGGTCGGCTTTCGGATCCCGCAGTCGAATCCGGTCGCGCGGCGTATCGAGAACCGGATTCC
GGGTGTCGACTGCAACCCGTATCTCGCGTTCGCGGCGACGCTCGCGGCCGGCTATCTCGGTCTCGAACAGCAGCTCGCGC
CGACCGAGCCGATCGCCTCGGACGGCTACTCGTTGCCGTACCAGCTGCCGCGCAATCTGGAGGAGGGCATTTCGTTGATG
GCCGCGTGCACGCCGCTCGCGGAGATTCTCGGCGACAAGTTCGTGAAGGCGTATCTCGCGCTGAAGGAAACCGAATACGA
AGCGTTCTTCCGCGTGATCAGCTCGTGGGAGCGCCGCCATCTGCTGCTGCACGTATGA

Upstream 100 bases:

>100_bases
CGGCGCGGCATGCCGCGCGCGAATGGCGCATCGCATTCATGCGGTCGGCGGCGAGTTGCCGTCGCCGAACGCGTCCGACG
TCGACTGAAAGAGGCCGATC

Downstream 100 bases:

>100_bases
GCGTGCGGCATCGTCATCCGGAGGCAACATGAACGATCGTCACGACGCACGGCCGAGCGCGCGCTCCACCGCCGAATACC
GCGCGCTCGACGCCGCGCAC

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQPELTEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLELYKQKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMEGEPGSAMHIHQSLADARTGRNLFADEGGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSNPVARRIENRIPGVDCNPYLAFAATLAAGYLGLEQQLAPTEPIASDGYSLPYQLPRNLEEGISLM
AACTPLAEILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MQPELTEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLELYKQKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMEGEPGSAMHIHQSLADARTGRNLFADEGGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSNPVARRIENRIPGVDCNPYLAFAATLAAGYLGLEQQLAPTEPIASDGYSLPYQLPRNLEEGISLM
AACTPLAEILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MQPELTEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLELYKQKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMEGEPGSAMHIHQSLADARTGRNLFADEGGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSNPVARRIENRIPGVDCNPYLAFAATLAAGYLGLEQQLAPTEPIASDGYSLPYQLPRNLEEGISLM
AACTPLAEILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=437, Percent_Identity=26.7734553775744, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=35.4120267260579, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=29.3736501079914, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49927; Mature: 49927

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPELTEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLE
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYKQKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFE
HHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMEGEPGSAMHIHQSLADARTGRNLFADEGGAVSPMFHSYLAGLQKYTPALMPIFA
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSNPVARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGLEQQLAPTEPIASDGYSLPYQLPRNLEEGISLMAACTPLAEILGDKFVKAYLA
HHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQPELTEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLE
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYKQKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFE
HHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMEGEPGSAMHIHQSLADARTGRNLFADEGGAVSPMFHSYLAGLQKYTPALMPIFA
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSNPVARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGLEQQLAPTEPIASDGYSLPYQLPRNLEEGISLMAACTPLAEILGDKFVKAYLA
HHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]