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The map label for this gene is nuoM [H]

Identifier: 161524216

GI number: 161524216

Start: 1136576

End: 1138066

Strand: Direct

Name: nuoM [H]

Synonym: Bmul_1040

Alternate gene names: 161524216

Gene position: 1136576-1138066 (Clockwise)

Preceding gene: 161524215

Following gene: 161524217

Centisome position: 32.96

GC content: 63.25

Gene sequence:

>1491_bases
ATGCACGCTTTTCCGATTCTCAGTACCGCGATCTGGCTGCCGATCGTTTTCGGCCTCCTCGTGCTCGCGGTCGGTAACGA
CAAGAACCCGGGGCCGGCACGCTGGATCGCGCTGATCGGCTCGCTGCTCGGGCTCGCCGTCACGATCCCGCTGATCACGG
GCTTCGATGCGAGCACGGCCGCACTGCAGTTCGTCGAGCAATCGACGTGGATCGAACGCTTCCAGATTTCGTATCACCTC
GGCGTCGACGGCATCTCGATGTGGTTCGTCGTGCTGACCGCGCTGATCACGGTGATCGTCGTGATCGCCGCGTGGGAAGT
GATCACCGAGAACGTCGCGCAGTACCTCGCCGCGTTCCTGATCCTGTCCGGCATCATGATCGGCGTGTTCTCGGCGGCCG
ACGGCCTGCTGTTCTACGTGTTCTTCGAGGCGACGCTGATCCCGATGTACATCATCATCGGCGTGTGGGGCGGCCCGAAC
CGCGTGTACGCGGCGTTCAAGTTCTTCCTGTACACGCTGGCCGGCTCGCTGCTGATGCTGGTCGCGCTGATCTACCTGTA
CACCGAGACGCACTCGTTCGACCTCGCGACGTGGCAGAACGCGAAGATCGCCATGACGCCGCAGATCCTGCTGTTCATCG
CCTTCTTCCTCGCGTTCGCGGTGAAGGTGCCGATGTGGCCGGTCCACACCTGGCTGCCGGATGCGCACGTGGAAGCGCCG
ACGGGCGGCTCGGTCGTGCTGGCGGCGATCATGCTGAAGCTCGGCGCGTACGGTTTCCTGCGATTCTCGCTTCCGATCAC
GCCTGACGCGAGCCACTTCCTCGCACCGGTCGTGATCACGCTGTCGCTGATCGCGGTGATCTACATCGGCCTCGTCGCGA
TGGTGCAGGCCGACATGAAGAAGCTGGTCGCGTATTCGTCGATCGCGCACATGGGCTTCGTGACGCTCGGCTTCTTCATC
TTCAACCAGCTCGGCGTCGAAGGCGCGATCGTGCAGATGATCTCGCACGGTTTCGTGTCGGGCGCGATGTTCCTCTGCAT
CGGCGTGCTGTACGACCGCGTGCACTCGCGCCAGATCGCCGACTACGGCGGCGTCGTCAACGTGATGCCGAAGTTCGCGG
CGTTCGCGATGCTGTTCTCGATGGCGAACTGCGGCCTGCCGGGCACGTCCGGTTTCGTCGGCGAGTTCATGGTGATTCTC
GCGGCCGTCCAGTACAACTTCTGGATTGCCTTCGGCGCGGCGTTCACGCTGATCCTCGGCGCGGCCTACACGCTGTGGAT
GTACAAGCGCGTCTACTTCGGCGCGGTCGCGAACGATCACGTCGCGAAGCTGAAGGACATCGGCCGCCGCGAATTCCTGA
TGCTCGCCGTGCTTGCCGCGTTCACGCTGCTGATGGGCCTGTATCCGAAGCCCTTCACCGACGTGATGCACGTTTCCGTG
GAAAACCTCCTCTCCCATGTCGCGCAGTCGAAGCTGCCGCTGGCCCAGTAA

Upstream 100 bases:

>100_bases
GGTTACATCTATCACTACGCGTTCGCCATGATCATCGGCATGCTGGGGCTCCTGACCCTGTTTGTAACGCTCGGCGGCAA
ATAAGGCGGGGGGACAACTA

Downstream 100 bases:

>100_bases
TCGCGAGCGGAGGAAATCAAGATCATGAACGCTCCTATGAATGTCCTGTTGCCTGACGCGCTGGTGATGGCCGCCATCAT
CGTCGCTTGGCTGAACGACA

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGPARWIALIGSLLGLAVTIPLITGFDASTAALQFVEQSTWIERFQISYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ

Sequences:

>Translated_496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGPARWIALIGSLLGLAVTIPLITGFDASTAALQFVEQSTWIERFQISYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ
>Mature_496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGPARWIALIGSLLGLAVTIPLITGFDASTAALQFVEQSTWIERFQISYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=371, Percent_Identity=34.5013477088949, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=24.5524296675192, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1788613, Length=469, Percent_Identity=39.8720682302772, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1788827, Length=379, Percent_Identity=23.7467018469657, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=25, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788829, Length=333, Percent_Identity=28.5285285285285, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693160, Length=451, Percent_Identity=24.1685144124169, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788614, Length=383, Percent_Identity=26.6318537859008, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54166; Mature: 54166

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGPARWIALIGSLLGLAVTIPLITGFDASTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALQFVEQSTWIERFQISYHLGVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFL
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIVQMISHGFVSGAMFLCIGVLYDRVHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAFGAAFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLAQ
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGPARWIALIGSLLGLAVTIPLITGFDASTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALQFVEQSTWIERFQISYHLGVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFL
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIVQMISHGFVSGAMFLCIGVLYDRVHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAFGAAFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLAQ
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]