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The map label for this gene is ilvD2 [H]

Identifier: 161524011

GI number: 161524011

Start: 908446

End: 910119

Strand: Reverse

Name: ilvD2 [H]

Synonym: Bmul_0832

Alternate gene names: 161524011

Gene position: 910119-908446 (Counterclockwise)

Preceding gene: 161524014

Following gene: 161524010

Centisome position: 26.39

GC content: 67.03

Gene sequence:

>1674_bases
ATGTCGTACAACCGTCGCTCGAAGCACATCACGCAAGGCGTCGCCCGCTCGCCGAACCGTTCGATGTATTACGCCCTCGG
CTATCAGAAGGAAGATTTCGACAAGCCGATGATCGGCATCGCCAACGGGCATTCGACGATCACGCCGTGCAACGCGGGGC
TGCAGCGCCTGTCCGACGCGGCCGTCGAGGCGATCAAGGCGGCCGGCGCGAATCCGCAGATCTTCGGCACGCCCACGATC
TCGGACGGCATGTCGATGGGCACCGAAGGGATGAAGTACTCGCTCGTGTCGCGCGAGGTAATCGCCGACTGCATCGAGAC
CTGCGTGCAGGGCCAATGGATGGACGGCGTCGTGGTAGTCGGCGGCTGCGACAAGAACATGCCGGGCGGGATGATCGCGC
TCGCGCGGCTGAACGTGCCGGGCATCTACGTGTACGGCGGGACGATCCGCCCCGGCCACTGGAAAGGCAAGGATCTGACG
ATCGTCTCGGCGTTCGAGGCGGTCGGCGAATTCACGGCCGGGCGCATGTCGGACGAGGATTTCGAGGGCATCGAGCGCAA
CGCGTGCCCGACGTCGGGCTCGTGCGGCGGCATGTACACCGCGAACACGATGAGCTCGTCGTTCGAGGCGCTCGGCATGT
CGCTGATGTATTCGTCGACGATGGCGAACCCCGATCAGGAAAAGGTCGATTCGGCGGCCGAATCGGCGCGTGTGCTCGTC
GAAGCGGTGAAGCGCGACCTGAAGCCGCGCGACATCATCACGAAGCAGTCGATCGAGAACGCGGTGTCGCTGATCATGGC
GACCGGCGGCTCGACCAATGCGGTGCTGCACTATCTCGCGATCGCGCATGCGGCCGAGGTCGACTGGACGATCGACGACT
TCGAGCGGATCCGCAAGCGCGTGCCGGTGATCTGCGACCTGAAGCCGTCGGGCCAGTACGTCGCGACCGACCTGCACCGT
GCGGGCGGCATTCCGCAGGTGCTGAAGATCCTGCTCGATGCGGGCCTGCTGCACGGCGACTGCGTCACGATCACCGGCCG
CACGCTCGCCGAAGAGCTGAAGGACGTGCCGTCCGTGCCGCGCGCCGATCAGCAGGTGATCTTCCCGATCGAGCGCGCGC
TGTACAAGGAGGGCCATCTCGCGATCCTGAAGGGCAATCTCGCCGAGGACGGCGCGGTCGCGAAGATCACCGGCCTGAAG
AACCCGGTGATCACGGGCCCGGCGCGCGTGTTCGACGACGAGCAAAGCGCGATGGACGCGATCCTCGGCGACAAGATCCG
CGCCGGCGACGTGCTCGTGCTGCGCTATCTCGGCCCGCAAGGCGGGCCCGGCATGCCGGAAATGCTCGCGCCGACGTCGG
CGATCATCGGCAAGGGGCTCGGCGAATCGGTCGGCTTCATCACCGACGGCCGCTTCTCCGGCGGTACGTGGGGGATGGTC
GTCGGCCACGTCGCGCCCGAGGCGTTCGTCGGCGGCACGATCGCGCTCGTGCAGGAAGGCGACTCGATCACGATCGACGC
GCATAAGCTGCTGCTGCAGCTGAACGTCGACGATGCGGAACTCGCGCGCCGCCGCGCCGCATGGAAGCAGCCGGCGCCGC
GCTACACGCGCGGCGTGCTCGCCAAGTATGCCGCGCTCGCACGGCCGGCGAATCGGGGTGCGGTCACGGGGTGA

Upstream 100 bases:

>100_bases
ATTGGACACGCGAAAACGGAAACGCCATCCTTGTTCGAGCCGCACCGTACGGCGCGTCCGCGCGATTGGTCCGGCCCCAC
AACAATCGATGGAGCTCCCC

Downstream 100 bases:

>100_bases
CGTGCGGCCGCGTCATGCGGGCTGTTTTCGGCGGTGCGGCCGTGCGGCCGCTCGGCGGTGCAGCGGTCCGTCCATCCCCG
CCCCACCGGCAGCGCGCCGA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSYNRRSKHITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGHSTITPCNAGLQRLSDAAVEAIKAAGANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGHWKGKDLT
IVSAFEAVGEFTAGRMSDEDFEGIERNACPTSGSCGGMYTANTMSSSFEALGMSLMYSSTMANPDQEKVDSAAESARVLV
EAVKRDLKPRDIITKQSIENAVSLIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHR
AGGIPQVLKILLDAGLLHGDCVTITGRTLAEELKDVPSVPRADQQVIFPIERALYKEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMDAILGDKIRAGDVLVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDAELARRRAAWKQPAPRYTRGVLAKYAALARPANRGAVTG

Sequences:

>Translated_557_residues
MSYNRRSKHITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGHSTITPCNAGLQRLSDAAVEAIKAAGANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGHWKGKDLT
IVSAFEAVGEFTAGRMSDEDFEGIERNACPTSGSCGGMYTANTMSSSFEALGMSLMYSSTMANPDQEKVDSAAESARVLV
EAVKRDLKPRDIITKQSIENAVSLIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHR
AGGIPQVLKILLDAGLLHGDCVTITGRTLAEELKDVPSVPRADQQVIFPIERALYKEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMDAILGDKIRAGDVLVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDAELARRRAAWKQPAPRYTRGVLAKYAALARPANRGAVTG
>Mature_556_residues
SYNRRSKHITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGHSTITPCNAGLQRLSDAAVEAIKAAGANPQIFGTPTIS
DGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGHWKGKDLTI
VSAFEAVGEFTAGRMSDEDFEGIERNACPTSGSCGGMYTANTMSSSFEALGMSLMYSSTMANPDQEKVDSAAESARVLVE
AVKRDLKPRDIITKQSIENAVSLIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHRA
GGIPQVLKILLDAGLLHGDCVTITGRTLAEELKDVPSVPRADQQVIFPIERALYKEGHLAILKGNLAEDGAVAKITGLKN
PVITGPARVFDDEQSAMDAILGDKIRAGDVLVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMVV
GHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDAELARRRAAWKQPAPRYTRGVLAKYAALARPANRGAVTG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=604, Percent_Identity=38.9072847682119, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI2367371, Length=491, Percent_Identity=34.6232179226069, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786464, Length=515, Percent_Identity=34.1747572815534, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788157, Length=483, Percent_Identity=31.055900621118, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=46.0714285714286, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59266; Mature: 59134

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNRRSKHITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGHSTITPCNAGLQRLSDA
CCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVEAIKAAGANPQIFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARLNVPGIYVYGGTIRPGHWKGKDLTIVSAFEAVGEFTAGRMSDED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCH
FEGIERNACPTSGSCGGMYTANTMSSSFEALGMSLMYSSTMANPDQEKVDSAAESARVLV
HCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKRDLKPRDIITKQSIENAVSLIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKR
HHHHHCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VPVICDLKPSGQYVATDLHRAGGIPQVLKILLDAGLLHGDCVTITGRTLAEELKDVPSVP
CCEEEECCCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
RADQQVIFPIERALYKEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMDA
CCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILGDKIRAGDVLVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHCCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDAELARRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHH
AKYAALARPANRGAVTG
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYNRRSKHITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGHSTITPCNAGLQRLSDA
CCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVEAIKAAGANPQIFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARLNVPGIYVYGGTIRPGHWKGKDLTIVSAFEAVGEFTAGRMSDED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCH
FEGIERNACPTSGSCGGMYTANTMSSSFEALGMSLMYSSTMANPDQEKVDSAAESARVLV
HCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKRDLKPRDIITKQSIENAVSLIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKR
HHHHHCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VPVICDLKPSGQYVATDLHRAGGIPQVLKILLDAGLLHGDCVTITGRTLAEELKDVPSVP
CCEEEECCCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
RADQQVIFPIERALYKEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMDA
CCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILGDKIRAGDVLVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHCCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDAELARRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHH
AKYAALARPANRGAVTG
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA