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The map label for this gene is lptD [H]

Identifier: 161523768

GI number: 161523768

Start: 642019

End: 644379

Strand: Direct

Name: lptD [H]

Synonym: Bmul_0588

Alternate gene names: 161523768

Gene position: 642019-644379 (Clockwise)

Preceding gene: 161523765

Following gene: 161523769

Centisome position: 18.62

GC content: 66.33

Gene sequence:

>2361_bases
ATGCCGCCCAAACCGCTATTCCCGAATGTCTTCCCCGCTGACGGGGCGCCGCGCAAACGGCGGCTCGCGCTTGCACTGCT
GGCCGTGCCCGGCCTCGTGCCGGCCGTGTCGCACGCGCAGCTGACGGGCGCGGCCGCGCAGCCGCAGCCGCTCGATTCGC
CGTGGGATCTGCGTCTCGCGCCGCAGCTCGAGGAACATCCGCTGAAGGACGGCGCGAAGCCCGCCGCGTTCGTGATCGCC
GACCACATGAGCGGCACGGCCGAGCAGGACATGGCCGCGAAAGGCTCGGCCGAACTGCGGCGCGGCGACGCGGTCGTGAA
AGCCGACGCGATCCACTACGATCAGGATACCGACATGGCCGATGCATACGGCCAGGTCAGGGTGATCAACGGCGGCACGT
CGTTCGCGGGCCCCGAAGCGCACCTGAAGATCGAGGCGAGCCAGGGCTTCATGACGGCGCCGAAGTATCACTTCAACGTG
ACGGGCGGCTCCGGCAGCGCCGAGCGCGTCGACATGCTCGACAGCGAGCGCTCGGTGTTCGTGAACGGCACGTACACCGC
GTGCGCGTGCGCGACGCATCCCGCGTGGTACATCAAGGGCAGCCGCTTCGACTTCGACACGGGCGCCGACGAAGGCACCG
CGCGCAACGGCGTGCTGTTCTTCCAGGGCGTGCCGATCTTCGCGAGCCCGTGGATGACGTTCCCGCTCTCGGGCGATCGC
AGAAGCGGCCTGCTGCCGCCGACGTTCTCGATGAACTCGAACAACGGGTTCGAGCTGTCGCTGCCGTACTACTTCAACAT
CGCGCCGAACCGCGACCTGACGATCACGCCGCGCATCATCTCGCGGCGCGGCGTGCAGACCGAAGCGACGTTCCGCTATC
TGTCGCCGAGCTATTCGGGCACGTTCACCGCGAACTACCTGCCGGACGACCGGCTCGCGCACCGCAACCGCTACGCGATC
TACTGGCAGCACCAGCAGAACTTCGGCGGCGGCTTCGGCGGCTACGTCTACTACAACAAGGTCTCGGACAATACCTATCC
CGAAGACCTTGGGTCGATGAACCAGTTCATCAACGGGACGCAGACGCTCTATCAGCAGGAAGCGGGCCTCACGTACAACA
AGGGCCCGTGGTCGGTGCTCGCGCGCTACCAGCACTGGCAGACGCTGCCGCCGTCGATCGCGCCGTACAGCCGCGAACCG
CAGTTGAACGTGAAGTACACGAAGTACAACGTCGGCGGCTTCGACTTCGGCGCGGAAGCCGACTACTCGCGGTTCCGCAT
CACGACCGCCGACTCGACGCAGGGCGACCGGATCGTCTTCAACCCGTACATCGCGTACGGCGTGTACGGCCCCGGCTACT
TCGTCGTGCCGAAGGTGCAGTACCACTTCGCGTCGTACGACCTGAACTATCTGTCGTCGAGCACGCCGAACAGCCCGAAG
CGCTTCACCGAATCGATTCCGACCGTCAGCTTCGACACCGGCCTGATCTTCGACCGCTCGGTGCGCCTGTTCGGCCAGGA
CTTCATCCAGACGCTCGAGCCGCGGCTCTACTACGTGTATACGCCGTACCGCGACCAGTCGAACGCGCCGCTGTTCGATA
CCGCGGAATCCGACTTCGGCCTCGCGGAGATCTACCAGCCGAACACGTTCGTCGGCAACGACCGCGTCGCGGACGCGAAC
CGGATCACGGCCGGCCTGACCTCGCGCTTCATCGATCCGCGCACCGGCGACGAGCGCGCGCGCTTCGTGATCGCGCAGCA
GTACTACTTCGCCGATCAGCGCGTGACGCTGAACACCGGGCAGTCCGCCGCGCAGGCGCGCCACTCCGACCTGATCGTCG
GCGCTGCGCTGAAGCTCGGTTCGGGCTTCATGTCGGAAACGGCGTTCCAGTACAACCAGAACAACAACCAGCTCGTGAAG
TCGAGCATCGGTTTCGGCTTCAGTCCCGGCGAGCGCAAGGTGATCAACGCCGCGTACCGCTACACGCGCGCCAGCACGAC
GCTCGACGGACAGCCGATCAACCAGTTCCTGGTGTCCGCGCAATGGCCGCTCACGCGGCGCCTGTACGCGGTCGGCCGCT
TCAACTACGATCTGGCCGGCGACCGGCTTGTCGACGGTCTGCTCGGCTTACAATACGACGCGGATTGCTGGGCGCTCGGC
GTCGGCGTGCAGCGGTTCGCCAACGGCGTGAATTCGTCCGGGCAGCGGAACTCGTCGACGCGCTTCATGATGCAGCTCAC
GCTCAAGGGGCTGTCGACCGTCGACAACGGCCTCGTCGCGGCGTTCCGCGCCGGCGTGCCGGGCTACACGCCGCTGCCGT
CGGCACCGCCGCCGATGTCCCGCTTCAGCAACTACGAGTAA

Upstream 100 bases:

>100_bases
TCGCCTCCCCGGTTTGCGCTCAGCCGCAGTCGCGGTTCGACGGCGCAGGCAGCCGTGCGTCGAAGGCGCCGTTGACAAGG
GGCGATTCGCCAAACGATAG

Downstream 100 bases:

>100_bases
CGCGGCCTGTCTGACCGGTCAGCCGATTTCAATGGAGTATCAGTGGCAATGAAGAAAACCCTTCGTTTCGCGGCAGTCGT
GTCCAGCCTCGCCGCGTCCG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MPPKPLFPNVFPADGAPRKRRLALALLAVPGLVPAVSHAQLTGAAAQPQPLDSPWDLRLAPQLEEHPLKDGAKPAAFVIA
DHMSGTAEQDMAAKGSAELRRGDAVVKADAIHYDQDTDMADAYGQVRVINGGTSFAGPEAHLKIEASQGFMTAPKYHFNV
TGGSGSAERVDMLDSERSVFVNGTYTACACATHPAWYIKGSRFDFDTGADEGTARNGVLFFQGVPIFASPWMTFPLSGDR
RSGLLPPTFSMNSNNGFELSLPYYFNIAPNRDLTITPRIISRRGVQTEATFRYLSPSYSGTFTANYLPDDRLAHRNRYAI
YWQHQQNFGGGFGGYVYYNKVSDNTYPEDLGSMNQFINGTQTLYQQEAGLTYNKGPWSVLARYQHWQTLPPSIAPYSREP
QLNVKYTKYNVGGFDFGAEADYSRFRITTADSTQGDRIVFNPYIAYGVYGPGYFVVPKVQYHFASYDLNYLSSSTPNSPK
RFTESIPTVSFDTGLIFDRSVRLFGQDFIQTLEPRLYYVYTPYRDQSNAPLFDTAESDFGLAEIYQPNTFVGNDRVADAN
RITAGLTSRFIDPRTGDERARFVIAQQYYFADQRVTLNTGQSAAQARHSDLIVGAALKLGSGFMSETAFQYNQNNNQLVK
SSIGFGFSPGERKVINAAYRYTRASTTLDGQPINQFLVSAQWPLTRRLYAVGRFNYDLAGDRLVDGLLGLQYDADCWALG
VGVQRFANGVNSSGQRNSSTRFMMQLTLKGLSTVDNGLVAAFRAGVPGYTPLPSAPPPMSRFSNYE

Sequences:

>Translated_786_residues
MPPKPLFPNVFPADGAPRKRRLALALLAVPGLVPAVSHAQLTGAAAQPQPLDSPWDLRLAPQLEEHPLKDGAKPAAFVIA
DHMSGTAEQDMAAKGSAELRRGDAVVKADAIHYDQDTDMADAYGQVRVINGGTSFAGPEAHLKIEASQGFMTAPKYHFNV
TGGSGSAERVDMLDSERSVFVNGTYTACACATHPAWYIKGSRFDFDTGADEGTARNGVLFFQGVPIFASPWMTFPLSGDR
RSGLLPPTFSMNSNNGFELSLPYYFNIAPNRDLTITPRIISRRGVQTEATFRYLSPSYSGTFTANYLPDDRLAHRNRYAI
YWQHQQNFGGGFGGYVYYNKVSDNTYPEDLGSMNQFINGTQTLYQQEAGLTYNKGPWSVLARYQHWQTLPPSIAPYSREP
QLNVKYTKYNVGGFDFGAEADYSRFRITTADSTQGDRIVFNPYIAYGVYGPGYFVVPKVQYHFASYDLNYLSSSTPNSPK
RFTESIPTVSFDTGLIFDRSVRLFGQDFIQTLEPRLYYVYTPYRDQSNAPLFDTAESDFGLAEIYQPNTFVGNDRVADAN
RITAGLTSRFIDPRTGDERARFVIAQQYYFADQRVTLNTGQSAAQARHSDLIVGAALKLGSGFMSETAFQYNQNNNQLVK
SSIGFGFSPGERKVINAAYRYTRASTTLDGQPINQFLVSAQWPLTRRLYAVGRFNYDLAGDRLVDGLLGLQYDADCWALG
VGVQRFANGVNSSGQRNSSTRFMMQLTLKGLSTVDNGLVAAFRAGVPGYTPLPSAPPPMSRFSNYE
>Mature_785_residues
PPKPLFPNVFPADGAPRKRRLALALLAVPGLVPAVSHAQLTGAAAQPQPLDSPWDLRLAPQLEEHPLKDGAKPAAFVIAD
HMSGTAEQDMAAKGSAELRRGDAVVKADAIHYDQDTDMADAYGQVRVINGGTSFAGPEAHLKIEASQGFMTAPKYHFNVT
GGSGSAERVDMLDSERSVFVNGTYTACACATHPAWYIKGSRFDFDTGADEGTARNGVLFFQGVPIFASPWMTFPLSGDRR
SGLLPPTFSMNSNNGFELSLPYYFNIAPNRDLTITPRIISRRGVQTEATFRYLSPSYSGTFTANYLPDDRLAHRNRYAIY
WQHQQNFGGGFGGYVYYNKVSDNTYPEDLGSMNQFINGTQTLYQQEAGLTYNKGPWSVLARYQHWQTLPPSIAPYSREPQ
LNVKYTKYNVGGFDFGAEADYSRFRITTADSTQGDRIVFNPYIAYGVYGPGYFVVPKVQYHFASYDLNYLSSSTPNSPKR
FTESIPTVSFDTGLIFDRSVRLFGQDFIQTLEPRLYYVYTPYRDQSNAPLFDTAESDFGLAEIYQPNTFVGNDRVADANR
ITAGLTSRFIDPRTGDERARFVIAQQYYFADQRVTLNTGQSAAQARHSDLIVGAALKLGSGFMSETAFQYNQNNNQLVKS
SIGFGFSPGERKVINAAYRYTRASTTLDGQPINQFLVSAQWPLTRRLYAVGRFNYDLAGDRLVDGLLGLQYDADCWALGV
GVQRFANGVNSSGQRNSSTRFMMQLTLKGLSTVDNGLVAAFRAGVPGYTPLPSAPPPMSRFSNYE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=775, Percent_Identity=26.8387096774194, Blast_Score=251, Evalue=9e-68,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR007543 [H]

Pfam domain/function: PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 86911; Mature: 86780

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPKPLFPNVFPADGAPRKRRLALALLAVPGLVPAVSHAQLTGAAAQPQPLDSPWDLRLA
CCCCCCCCCCCCCCCCCCHHHHEEHHHHHCCHHHCCCCCEECCCCCCCCCCCCCCCEEEC
PQLEEHPLKDGAKPAAFVIADHMSGTAEQDMAAKGSAELRRGDAVVKADAIHYDQDTDMA
CCHHHCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCCCHH
DAYGQVRVINGGTSFAGPEAHLKIEASQGFMTAPKYHFNVTGGSGSAERVDMLDSERSVF
HCCCEEEEEECCCCCCCCCEEEEEEECCCCEECCEEEEEEECCCCCCHHHHHCCCCCEEE
VNGTYTACACATHPAWYIKGSRFDFDTGADEGTARNGVLFFQGVPIFASPWMTFPLSGDR
EECCEEEEEECCCCEEEEECCEECCCCCCCCCCCCCCEEEEECCCEEECCCEEECCCCCC
RSGLLPPTFSMNSNNGFELSLPYYFNIAPNRDLTITPRIISRRGVQTEATFRYLSPSYSG
CCCCCCCEEECCCCCCEEEECCEEEEECCCCCEEECHHHHHCCCCCCHHEEEEECCCCCC
TFTANYLPDDRLAHRNRYAIYWQHQQNFGGGFGGYVYYNKVSDNTYPEDLGSMNQFINGT
EEEECCCCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCHH
QTLYQQEAGLTYNKGPWSVLARYQHWQTLPPSIAPYSREPQLNVKYTKYNVGGFDFGAEA
HHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
DYSRFRITTADSTQGDRIVFNPYIAYGVYGPGYFVVPKVQYHFASYDLNYLSSSTPNSPK
CCCEEEEEECCCCCCCEEEECCEEEEEECCCCEEEEEEEEEEEEEEEHHHHCCCCCCCHH
RFTESIPTVSFDTGLIFDRSVRLFGQDFIQTLEPRLYYVYTPYRDQSNAPLFDTAESDFG
HHHHHCCCEEECCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCC
LAEIYQPNTFVGNDRVADANRITAGLTSRFIDPRTGDERARFVIAQQYYFADQRVTLNTG
EEEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECC
QSAAQARHSDLIVGAALKLGSGFMSETAFQYNQNNNQLVKSSIGFGFSPGERKVINAAYR
CHHHHHHHCCEEEEEHHHHCCCHHHHHHEEECCCCCEEEHHHCCCCCCCCCHHHHHHHHH
YTRASTTLDGQPINQFLVSAQWPLTRRLYAVGRFNYDLAGDRLVDGLLGLQYDADCWALG
HEECCCCCCCCCHHHHHEECCCCHHHEEEEEECCCCCCCCHHHHHHHHCCEECCCCEEEH
VGVQRFANGVNSSGQRNSSTRFMMQLTLKGLSTVDNGLVAAFRAGVPGYTPLPSAPPPMS
HHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHH
RFSNYE
HCCCCC
>Mature Secondary Structure 
PPKPLFPNVFPADGAPRKRRLALALLAVPGLVPAVSHAQLTGAAAQPQPLDSPWDLRLA
CCCCCCCCCCCCCCCCCHHHHEEHHHHHCCHHHCCCCCEECCCCCCCCCCCCCCCEEEC
PQLEEHPLKDGAKPAAFVIADHMSGTAEQDMAAKGSAELRRGDAVVKADAIHYDQDTDMA
CCHHHCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCCCHH
DAYGQVRVINGGTSFAGPEAHLKIEASQGFMTAPKYHFNVTGGSGSAERVDMLDSERSVF
HCCCEEEEEECCCCCCCCCEEEEEEECCCCEECCEEEEEEECCCCCCHHHHHCCCCCEEE
VNGTYTACACATHPAWYIKGSRFDFDTGADEGTARNGVLFFQGVPIFASPWMTFPLSGDR
EECCEEEEEECCCCEEEEECCEECCCCCCCCCCCCCCEEEEECCCEEECCCEEECCCCCC
RSGLLPPTFSMNSNNGFELSLPYYFNIAPNRDLTITPRIISRRGVQTEATFRYLSPSYSG
CCCCCCCEEECCCCCCEEEECCEEEEECCCCCEEECHHHHHCCCCCCHHEEEEECCCCCC
TFTANYLPDDRLAHRNRYAIYWQHQQNFGGGFGGYVYYNKVSDNTYPEDLGSMNQFINGT
EEEECCCCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCHH
QTLYQQEAGLTYNKGPWSVLARYQHWQTLPPSIAPYSREPQLNVKYTKYNVGGFDFGAEA
HHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
DYSRFRITTADSTQGDRIVFNPYIAYGVYGPGYFVVPKVQYHFASYDLNYLSSSTPNSPK
CCCEEEEEECCCCCCCEEEECCEEEEEECCCCEEEEEEEEEEEEEEEHHHHCCCCCCCHH
RFTESIPTVSFDTGLIFDRSVRLFGQDFIQTLEPRLYYVYTPYRDQSNAPLFDTAESDFG
HHHHHCCCEEECCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCC
LAEIYQPNTFVGNDRVADANRITAGLTSRFIDPRTGDERARFVIAQQYYFADQRVTLNTG
EEEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECC
QSAAQARHSDLIVGAALKLGSGFMSETAFQYNQNNNQLVKSSIGFGFSPGERKVINAAYR
CHHHHHHHCCEEEEEHHHHCCCHHHHHHEEECCCCCEEEHHHCCCCCCCCCHHHHHHHHH
YTRASTTLDGQPINQFLVSAQWPLTRRLYAVGRFNYDLAGDRLVDGLLGLQYDADCWALG
HEECCCCCCCCCHHHHHEECCCCHHHEEEEEECCCCCCCCHHHHHHHHCCEECCCCEEEH
VGVQRFANGVNSSGQRNSSTRFMMQLTLKGLSTVDNGLVAAFRAGVPGYTPLPSAPPPMS
HHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHH
RFSNYE
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA