BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is paaF [H]

Identifier: 161523761

GI number: 161523761

Start: 635232

End: 636002

Strand: Direct

Name: paaF [H]

Synonym: Bmul_0581

Alternate gene names: 161523761

Gene position: 635232-636002 (Clockwise)

Preceding gene: 161523760

Following gene: 161523763

Centisome position: 18.42

GC content: 68.09

Gene sequence:

>771_bases
ATGGTGGCCGACATTCAAGTGGAACGCGCCGACGGCGTACTGACGATCACGATCGCGCGTCCGGCGAAGAAGAATGCGCT
GACGGCGGCGATGTATCAGGCGATGGCCGATGCGCTCGCCGATGCGCAGGAAGACAGCGCGGTCCGCGCTATCCTGCTGC
GCGGCAGCGACGGCAATTTCAGCGCGGGCAACGATCTCGAGGATTTCCTGAAGGCGCCGCCGAAGGACGAGAACGCGCCG
GTGTTCCAGTTTCTCGCGCGCATCAGCGGCGCGAGCAAGCCGATCGTCGCCGCGGTGCCGGGCATCGCGGTCGGGGTCGG
CGTGACGATGCTGCTGCACTGCGATCTCGTCTACGCGGCCGATACCGCGACCTTCTCGCTGCCGTTCGTGCAGCTCGGGC
TGTGTCCCGAGGCCGCGTCGAGCATGCTGCTGCCGCGGCTCACAGGCCATCAGATCGCTGCGGAGAAGCTGCTGCTCGGC
GAACCGTTCGACGCGCTCGAGGCGCACCGCATCGGGATCGTCAATCGCGTGCTGCCGGCCGCGGAGCTCGATGCGTTCGC
CGCGAAGCAGGCCGCGAAGCTCGCGGCGCTGCCGGCCTCGTCGCTGCGCGTGACGAAGGCGCTGCTCAAGGATACGGGCG
GCGTGACGGTGGCCGCACGGATGGCCGAAGAGGCCGGCCATTTCTCGGCGATGCTGCGCGCGCCCGAAGCGCGCGAGGCG
ATGACGGCATTCTTCGAGAAGCGCAAACCCGATTTCCGCCAGTTCGACTGA

Upstream 100 bases:

>100_bases
GAACGCCTGTAAGCGGGACGCGACGAAACGCGACGACGGTGCGCGGGCGGCGAGCTCGCGCACCGTTTTTCATTCCGGTA
GGGGCAAGTCAAAGGAGACG

Downstream 100 bases:

>100_bases
CGCGCCGCGCGTGCCGGCCCGGCGACCGCGCCGCGGCCGGGCCGGCGCCGGCCGTCATGCGGTGCCGTAGTCGACGTAGC
CGGTTTCGCGGCAGAAGCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVADIQVERADGVLTITIARPAKKNALTAAMYQAMADALADAQEDSAVRAILLRGSDGNFSAGNDLEDFLKAPPKDENAP
VFQFLARISGASKPIVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFVQLGLCPEAASSMLLPRLTGHQIAAEKLLLG
EPFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPASSLRVTKALLKDTGGVTVAARMAEEAGHFSAMLRAPEAREA
MTAFFEKRKPDFRQFD

Sequences:

>Translated_256_residues
MVADIQVERADGVLTITIARPAKKNALTAAMYQAMADALADAQEDSAVRAILLRGSDGNFSAGNDLEDFLKAPPKDENAP
VFQFLARISGASKPIVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFVQLGLCPEAASSMLLPRLTGHQIAAEKLLLG
EPFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPASSLRVTKALLKDTGGVTVAARMAEEAGHFSAMLRAPEAREA
MTAFFEKRKPDFRQFD
>Mature_256_residues
MVADIQVERADGVLTITIARPAKKNALTAAMYQAMADALADAQEDSAVRAILLRGSDGNFSAGNDLEDFLKAPPKDENAP
VFQFLARISGASKPIVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFVQLGLCPEAASSMLLPRLTGHQIAAEKLLLG
EPFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPASSLRVTKALLKDTGGVTVAARMAEEAGHFSAMLRAPEAREA
MTAFFEKRKPDFRQFD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=249, Percent_Identity=32.9317269076305, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI260275230, Length=249, Percent_Identity=32.9317269076305, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI260274832, Length=249, Percent_Identity=32.9317269076305, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=30, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=32.5490196078431, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=27.6264591439689, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=27.6264591439689, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=26.8482490272374, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=26.8482490272374, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757966, Length=215, Percent_Identity=29.7674418604651, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI51538581, Length=215, Percent_Identity=29.7674418604651, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI68989263, Length=277, Percent_Identity=29.2418772563177, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=24.124513618677, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=24.124513618677, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=29.5698924731183, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=24.124513618677, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=28.3720930232558, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI150378541, Length=247, Percent_Identity=29.1497975708502, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=27.5590551181102, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=27.807486631016, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=27.807486631016, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI62530384, Length=234, Percent_Identity=23.5042735042735, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=31.7647058823529, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=32.319391634981, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=31.9230769230769, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=32.258064516129, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=29.6875, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790281, Length=257, Percent_Identity=26.8482490272374, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=29.0155440414508, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=249, Percent_Identity=36.9477911646586, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=32.03125, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=31.9066147859922, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=30.6122448979592, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=31.9796954314721, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=204, Percent_Identity=26.9607843137255, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=24.3801652892562, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=32.8638497652582, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=213, Percent_Identity=32.8638497652582, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=32.8638497652582, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=183, Percent_Identity=25.6830601092896, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=27.4111675126904, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323314, Length=199, Percent_Identity=27.6381909547739, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24654903, Length=246, Percent_Identity=30.8943089430894, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=30.7053941908714, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=30.7053941908714, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=32.4427480916031, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=25.0980392156863, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357171, Length=263, Percent_Identity=26.615969581749, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=27.3224043715847, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=27.1844660194175, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27041; Mature: 27041

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADIQVERADGVLTITIARPAKKNALTAAMYQAMADALADAQEDSAVRAILLRGSDGNF
CCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SAGNDLEDFLKAPPKDENAPVFQFLARISGASKPIVAAVPGIAVGVGVTMLLHCDLVYAA
CCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHEEE
DTATFSLPFVQLGLCPEAASSMLLPRLTGHQIAAEKLLLGEPFDALEAHRIGIVNRVLPA
CCCEEECCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
AELDAFAAKQAAKLAALPASSLRVTKALLKDTGGVTVAARMAEEAGHFSAMLRAPEAREA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHH
MTAFFEKRKPDFRQFD
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVADIQVERADGVLTITIARPAKKNALTAAMYQAMADALADAQEDSAVRAILLRGSDGNF
CCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SAGNDLEDFLKAPPKDENAPVFQFLARISGASKPIVAAVPGIAVGVGVTMLLHCDLVYAA
CCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHEEE
DTATFSLPFVQLGLCPEAASSMLLPRLTGHQIAAEKLLLGEPFDALEAHRIGIVNRVLPA
CCCEEECCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
AELDAFAAKQAAKLAALPASSLRVTKALLKDTGGVTVAARMAEEAGHFSAMLRAPEAREA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHH
MTAFFEKRKPDFRQFD
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]