Click here to switch to the map view.

The map label for this gene is ybhO [H]

Identifier: 161523756

GI number: 161523756

Start: 627397

End: 628671

Strand: Direct

Name: ybhO [H]

Synonym: Bmul_0576

Alternate gene names: 161523756

Gene position: 627397-628671 (Clockwise)

Preceding gene: 161523755

Following gene: 161523757

Centisome position: 18.19

GC content: 68.86

Gene sequence:

>1275_bases
ATGAATGCGGAAACCCGCAAGCGCTTCGCGCAGTTGCGGCAGATGTTCCTGCAGGAGCGCGGCTCCGCGTCGCGGCTCGC
GTTCACGACCGGCAACACGGTGCGCTTGTGCGAAGGCGGCGGCGAGTTCTTCCGCGCGCTGATCGACCGCATCGACGCCG
CGCGCGAGCAGGTCATGCTCGAAACCTACATCTTCTGCGACGACGCGGCCGGCCGGCCGGTGTCCGACGCGCTGATCCGC
GCCGCGCTGCGCGGCGTGCGCGTGCGCGTGATCACCGACGGCGTCGGCACCGCGCGCCTGCCGCTGTTCGACGCGTGGAC
CGAGGCCGGCATCGAGCACTGCATCTACAACCGCTATCTGTTCGGCCGTTTCGGTTTTTCGCGCACGCATCGCAAGCTTG
CCGTGATCGACCGGTCGGCCGCGTTCTGCGGCGGCATCAACATCGTCGACGATTACGAGCAGAACGGCGCGCCGCTGCAG
TATCCGCGCTGGGACTTCGCGCTCGAGCTCGCGGGGCCGGCCGTCGCCGACGTACGCGCGGCATTCGAGCTGCAGTGGCA
CCGCATTCAGTTCGGTCACAAGCCGTACGCGCAGTACGCGGCCGGGCTGCACGGCGGCGAGCCGTTCCCCGAGATGTTCC
GGCGCTGGATTCGCAGCCACCGCTGGATCAAGGCCGGCGCGCTGCGCGTCGTGACCGAGCCGAGCGTCGCGTTCGTCGCG
CGCGACAACGTCGTGAACCGGCGCACGATCGAGAAGGCGTATCTCGCGGCGATCGGCCGCGCGCGCCATCAGATCCGGCT
CGCCAATCCGTATTTCATGCCGGGCCGCAAGCTGCGCCGCGCGCTGACGGGCGCGGCGCGGCGCGGCGTCGACGTGCGGA
TTCTGGTCGGCCGCAAGGAATTCGCGGCGCTCGACACGGCCGTGCCGTTCCTCTATCGCGCGCTGCTGCGCGCCGGCGTG
CGCGTCGCCGAATACGACAAGACGATCCTGCACGGCAAGGTGGCTGTGATCGACGACAACTGGGCGACGGTCGGCTCGTC
GAATCTGGACGCGCTGAGCCTGATGCTGAACAATGAGGCCAACGTGGTGCTCGTGCGCTACGACGCGGTCACGAACGAGC
TGCGCGATGCGATCGAGGCGGCATTCGCGGACGGCCGCGAGATCGATCCCGCGTGGTACGCGGCGCGGCCGCTCGGCGAA
CGCTTGCTGAGCTGGCTCGCTTATACGACCTACCGGATCGTCATGAAGGCGCTGACGGTCGGCGGTTACGACTAG

Upstream 100 bases:

>100_bases
GCGACGCGGCCGCGCGCTGCTTCTCGCCGTCGAACGCCGACGCGATCCTGCAGCTTCCGGTGCGCGCGTCGCTCGTGCAC
CTGCCGCACGGCTCGTCCGC

Downstream 100 bases:

>100_bases
GCGAGCGCGGCGCGGTGCCGGCGCCGCAGCGCGTCGGCCGCCGCGCAGTACGCGCCGTGCACGGGCGCATACCATTTCAA
AACGTCCGCACGTTCGTGCG

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNAETRKRFAQLRQMFLQERGSASRLAFTTGNTVRLCEGGGEFFRALIDRIDAAREQVMLETYIFCDDAAGRPVSDALIR
AALRGVRVRVITDGVGTARLPLFDAWTEAGIEHCIYNRYLFGRFGFSRTHRKLAVIDRSAAFCGGINIVDDYEQNGAPLQ
YPRWDFALELAGPAVADVRAAFELQWHRIQFGHKPYAQYAAGLHGGEPFPEMFRRWIRSHRWIKAGALRVVTEPSVAFVA
RDNVVNRRTIEKAYLAAIGRARHQIRLANPYFMPGRKLRRALTGAARRGVDVRILVGRKEFAALDTAVPFLYRALLRAGV
RVAEYDKTILHGKVAVIDDNWATVGSSNLDALSLMLNNEANVVLVRYDAVTNELRDAIEAAFADGREIDPAWYAARPLGE
RLLSWLAYTTYRIVMKALTVGGYD

Sequences:

>Translated_424_residues
MNAETRKRFAQLRQMFLQERGSASRLAFTTGNTVRLCEGGGEFFRALIDRIDAAREQVMLETYIFCDDAAGRPVSDALIR
AALRGVRVRVITDGVGTARLPLFDAWTEAGIEHCIYNRYLFGRFGFSRTHRKLAVIDRSAAFCGGINIVDDYEQNGAPLQ
YPRWDFALELAGPAVADVRAAFELQWHRIQFGHKPYAQYAAGLHGGEPFPEMFRRWIRSHRWIKAGALRVVTEPSVAFVA
RDNVVNRRTIEKAYLAAIGRARHQIRLANPYFMPGRKLRRALTGAARRGVDVRILVGRKEFAALDTAVPFLYRALLRAGV
RVAEYDKTILHGKVAVIDDNWATVGSSNLDALSLMLNNEANVVLVRYDAVTNELRDAIEAAFADGREIDPAWYAARPLGE
RLLSWLAYTTYRIVMKALTVGGYD
>Mature_424_residues
MNAETRKRFAQLRQMFLQERGSASRLAFTTGNTVRLCEGGGEFFRALIDRIDAAREQVMLETYIFCDDAAGRPVSDALIR
AALRGVRVRVITDGVGTARLPLFDAWTEAGIEHCIYNRYLFGRFGFSRTHRKLAVIDRSAAFCGGINIVDDYEQNGAPLQ
YPRWDFALELAGPAVADVRAAFELQWHRIQFGHKPYAQYAAGLHGGEPFPEMFRRWIRSHRWIKAGALRVVTEPSVAFVA
RDNVVNRRTIEKAYLAAIGRARHQIRLANPYFMPGRKLRRALTGAARRGVDVRILVGRKEFAALDTAVPFLYRALLRAGV
RVAEYDKTILHGKVAVIDDNWATVGSSNLDALSLMLNNEANVVLVRYDAVTNELRDAIEAAFADGREIDPAWYAARPLGE
RLLSWLAYTTYRIVMKALTVGGYD

Specific function: Catalyzes, in vitro, the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=397, Percent_Identity=36.0201511335013, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1787502, Length=398, Percent_Identity=26.8844221105528, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47671; Mature: 47671

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAETRKRFAQLRQMFLQERGSASRLAFTTGNTVRLCEGGGEFFRALIDRIDAAREQVML
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
ETYIFCDDAAGRPVSDALIRAALRGVRVRVITDGVGTARLPLFDAWTEAGIEHCIYNRYL
HHEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
FGRFGFSRTHRKLAVIDRSAAFCGGINIVDDYEQNGAPLQYPRWDFALELAGPAVADVRA
HHCCCCCHHCCEEEEEECCHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHH
AFELQWHRIQFGHKPYAQYAAGLHGGEPFPEMFRRWIRSHRWIKAGALRVVTEPSVAFVA
HHHEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCCEEEEECCCEEEEE
RDNVVNRRTIEKAYLAAIGRARHQIRLANPYFMPGRKLRRALTGAARRGVDVRILVGRKE
CCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHH
FAALDTAVPFLYRALLRAGVRVAEYDKTILHGKVAVIDDNWATVGSSNLDALSLMLNNEA
HHHHHHHHHHHHHHHHHCCCEEHHHCHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCC
NVVLVRYDAVTNELRDAIEAAFADGREIDPAWYAARPLGERLLSWLAYTTYRIVMKALTV
CEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGYD
CCCC
>Mature Secondary Structure
MNAETRKRFAQLRQMFLQERGSASRLAFTTGNTVRLCEGGGEFFRALIDRIDAAREQVML
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
ETYIFCDDAAGRPVSDALIRAALRGVRVRVITDGVGTARLPLFDAWTEAGIEHCIYNRYL
HHEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
FGRFGFSRTHRKLAVIDRSAAFCGGINIVDDYEQNGAPLQYPRWDFALELAGPAVADVRA
HHCCCCCHHCCEEEEEECCHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHH
AFELQWHRIQFGHKPYAQYAAGLHGGEPFPEMFRRWIRSHRWIKAGALRVVTEPSVAFVA
HHHEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCCEEEEECCCEEEEE
RDNVVNRRTIEKAYLAAIGRARHQIRLANPYFMPGRKLRRALTGAARRGVDVRILVGRKE
CCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHH
FAALDTAVPFLYRALLRAGVRVAEYDKTILHGKVAVIDDNWATVGSSNLDALSLMLNNEA
HHHHHHHHHHHHHHHHHCCCEEHHHCHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCC
NVVLVRYDAVTNELRDAIEAAFADGREIDPAWYAARPLGERLLSWLAYTTYRIVMKALTV
CEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGYD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]