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The map label for this gene is aspS [H]

Identifier: 161523754

GI number: 161523754

Start: 624938

End: 626740

Strand: Direct

Name: aspS [H]

Synonym: Bmul_0574

Alternate gene names: 161523754

Gene position: 624938-626740 (Clockwise)

Preceding gene: 161523753

Following gene: 161523755

Centisome position: 18.12

GC content: 65.28

Gene sequence:

>1803_bases
ATGTCGATGCGTACTGAATACTGCGGTCTCGTGACCGAACACCTGCTGGGCCAAACCGTGTCGCTGTGCGGCTGGGTGCA
GCGCCGCCGCGATCACGGCGGTGTGATCTTCATCGACCTGCGCGATCGTGAAGGCCTGGTGCAGGTCGTGTGCGACCCGG
ATCGCGCCGAGATGTTCGCGACCGCCGAAGGCGTGCGCAACGAATTCTGCGTGCAGGTGAAGGGCCTCGTGCGCAACCGT
CCGGAAGGCACGGTGAACGCGGGCCTGAAGAGCGGCAAGATCGAGGTGCTCTGCCACGAGCTGAACGTGCTGAACCCGTC
GATCACGCCGCCGTTCCAGCTCGACGACGACAACCTGTCGGAAACGACGCGCCTCACGCACCGCGTGCTCGACCTGCGCC
GCCCGCAGATGCAGCACAACCTGCGCCTGCGCTACCGCGTCGCGATCGAAGCGCGCAAGTACCTCGACGAGCAAGGCTTC
ATCGACATCGAAACGCCGATGCTGACGAAGAGCACGCCGGAAGGCGCGCGCGACTACCTCGTGCCGTCGCGCGTGAACGC
AGGCCAGTTCTTCGCGCTGCCGCAGTCGCCGCAGCTGTTCAAGCAGCTGCTGATGGTCGCGAACTTCGACCGCTACTACC
AGATCACGAAGTGCTTCCGTGACGAAGACCTGCGCGCCGATCGCCAGCCGGAATTCACGCAGATCGACTGCGAGACGTCG
TTCCTCGGCGAGCAGGAGATCCGCGACCTGTTCGAGGACATGATCCGTCACATCTTCAAGACGACGATCAACGTCGAGCT
CGACGCGAAATTCCCGGTGATGCCGTACTCGGAAGCGATGGCGCGCTTCGGCTCCGACAAGCCGGACCTGCGCGTGAAGC
TCGAGTTCACCGAGCTGACCGATGCAATGAAGGACGTCGACTTCAAGGTGTTCAGCACGCCGGCCAACGCGAAGGACGGC
CGCGTCGCGGCGCTGCGCGTGCCGAAGGGCGCCGAGCTGTCGCGCGGCGACATCGACGGCTACACGGAATTCGTGCGCAT
CTACGGCGCGAAGGGTCTCGCGTGGATCAAGGTCAACGAGAAGGCGAAGGGTCGCGACGGCCTGCAGAGCCCGATCGTCA
AGAACCTGCACGACGCGTCGATCGCCGCGATTCTCGAGCGCACGGGCGCGGAAGACGGCGACATCATCTTCTTCGCGGCC
GATCGCGCGAAGGTCGTCAACGACAGTCTCGGCGCGCTGCGCCTGAAGATCGGTCATTCGGAGTTCGGCAAGGCGAACGG
CCTGGTCGAGGCGGGCTGGAAGCCGCTGTGGGTCGTCGACTTCCCGATGTTCGAATACGACGACGAAGACGCACGCTACG
TGGCCGCGCACCACCCGTTCACGAGCCCGAAGGACGAGCACCTCGAGTATCTCGAGAGCGATCCGGGCCGCTGCCTGGCG
AAGGCATACGACATGGTGCTGAACGGCTGGGAAATCGGCGGCGGCTCGGTGCGTATCCACCGCGAGGACGTGCAGAGCAA
GGTGTTCCGCGCGCTGAAGATCGGCGCGGAAGAAGCGCAGGCGAAGTTCGGCTTCCTGCTGGACGCGCTGCAATACGGCG
CGCCGCCGCACGGCGGTATCGCGTTCGGTCTCGACCGCATCGTCACGATGATGGCCGGCGCCGATTCGATCCGCGACGTG
ATCGCGTTCCCGAAGACGCAGCGTGCGCAGGATCTGCTCACGCAGGCGCCGAGCCCGGTCGACGAGCGTCAGCTGCGCGA
ACTGCACATCCGTCTGCGTCAGCCGGAGCAGCCGGCGTCGTAA

Upstream 100 bases:

>100_bases
CCCGCCCGCCGTCCCGTCGAGCCGCCGCTGTAAGCCAAGAATCATCGCCCGGGCCGCGCGTTGCGGCGCCCGTTGATCAA
ACCGAACGAAAGCAAACATC

Downstream 100 bases:

>100_bases
TGCGCGAACTGCATCGCGGCGTGTGCGGCCACGCGCACGCTGTCTGAAGAAAAAGGCGCCATCGTGCGCCTTTTTCGTTT
TTTGCGGTACAGTCGCAGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQVKGLVRNR
PEGTVNAGLKSGKIEVLCHELNVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLGEQEIRDLFEDMIRHIFKTTINVELDAKFPVMPYSEAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANAKDG
RVAALRVPKGAELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLESDPGRCLA
KAYDMVLNGWEIGGGSVRIHREDVQSKVFRALKIGAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPAS

Sequences:

>Translated_600_residues
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQVKGLVRNR
PEGTVNAGLKSGKIEVLCHELNVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLGEQEIRDLFEDMIRHIFKTTINVELDAKFPVMPYSEAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANAKDG
RVAALRVPKGAELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLESDPGRCLA
KAYDMVLNGWEIGGGSVRIHREDVQSKVFRALKIGAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPAS
>Mature_599_residues
SMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFATAEGVRNEFCVQVKGLVRNRP
EGTVNAGLKSGKIEVLCHELNVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFI
DIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSF
LGEQEIRDLFEDMIRHIFKTTINVELDAKFPVMPYSEAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANAKDGR
VAALRVPKGAELSRGDIDGYTEFVRIYGAKGLAWIKVNEKAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAAD
RAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLESDPGRCLAK
AYDMVLNGWEIGGGSVRIHREDVQSKVFRALKIGAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVI
AFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPAS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=38.2207578253707, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI45439306, Length=286, Percent_Identity=29.3706293706294, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194272210, Length=255, Percent_Identity=24.3137254901961, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI5031815, Length=255, Percent_Identity=24.3137254901961, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=57.3604060913706, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=262, Percent_Identity=25.5725190839695, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790571, Length=247, Percent_Identity=25.5060728744939, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=616, Percent_Identity=34.4155844155844, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17535927, Length=301, Percent_Identity=23.2558139534884, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=301, Percent_Identity=23.2558139534884, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=279, Percent_Identity=23.2974910394265, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=262, Percent_Identity=25.5725190839695, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=647, Percent_Identity=32.6120556414219, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6323011, Length=259, Percent_Identity=29.3436293436293, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=249, Percent_Identity=27.3092369477912, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=207, Percent_Identity=26.5700483091787, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=263, Percent_Identity=25.0950570342205, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=36.3486842105263, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=28.4090909090909, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640849, Length=254, Percent_Identity=26.3779527559055, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=254, Percent_Identity=26.3779527559055, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67673; Mature: 67542

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQVKGLVRNRPEGTVNAGLKSGKIEVLCHELNVLNPSITPPFQLDDDNLS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCEEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLGEQEIRDLFEDMIRHIFKTTINVELDAKFPVMPYSEAMARFGSDKPDLRVKLEFTELT
HCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANAKDGRVAALRVPKGAELSRGDIDGYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
KAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAADRAKVVNDSLGALRLKIGHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVEAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLESDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHH
KAYDMVLNGWEIGGGSVRIHREDVQSKVFRALKIGAEEAQAKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPAS
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SMRTEYCGLVTEHLLGQTVSLCGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQVKGLVRNRPEGTVNAGLKSGKIEVLCHELNVLNPSITPPFQLDDDNLS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCEEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEARKYLDEQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLGEQEIRDLFEDMIRHIFKTTINVELDAKFPVMPYSEAMARFGSDKPDLRVKLEFTELT
HCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANAKDGRVAALRVPKGAELSRGDIDGYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
KAKGRDGLQSPIVKNLHDASIAAILERTGAEDGDIIFFAADRAKVVNDSLGALRLKIGHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVEAGWKPLWVVDFPMFEYDDEDARYVAAHHPFTSPKDEHLEYLESDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHH
KAYDMVLNGWEIGGGSVRIHREDVQSKVFRALKIGAEEAQAKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDERQLRELHIRLRQPEQPAS
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA