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The map label for this gene is maiA [H]

Identifier: 161523671

GI number: 161523671

Start: 538121

End: 538765

Strand: Direct

Name: maiA [H]

Synonym: Bmul_0491

Alternate gene names: 161523671

Gene position: 538121-538765 (Clockwise)

Preceding gene: 161523668

Following gene: 161523673

Centisome position: 15.6

GC content: 64.65

Gene sequence:

>645_bases
ATGAAGCTCTACAGCTATTTCCGCAGTTCCGCTTCGTACCGCGTGCGGATCGCGCTGAACCTGAAGCGGCTGCCGTACGA
CTACGTGCCCGTGCACATGCTGCGCGACGGCGGCCAGCAACTGAAGGACGAGTATCGCGCGCTGAACCCCGACGCGCTCG
TGCCGACGCTCGTCGACGGCGACGCGACGCTGCAGCAGTCGCTCGCGATCATCGAGTATCTCGACGAAACGCATCCGGAG
CCCGCGTTGCTGCCGAAGGCGCCCGTCGATCGAGCGTACGTGCGTGCGATCGCGCTGCAGATCGCATGCGAGATCCATCC
GCTCGACAACCTGCGCGTGCTGAAGTACCTGAAGCACACGCTGAAGGTATCGGAGGATGACAAGAACGCCTGGTACCGGC
ACTGGATCGAGTCGGGCTTCGCGTCGCTCGAAACGCGCCTCGCGAACGATCCGCGCACCGGCAAGCTCTGCTTCGGCGAT
ACACCGACGCTCGCCGACATCTGCCTCGTGCCGCAGGTGTTCAACGCGAACCGCTTCTCGATCGACACGACGCGCTATCC
GACGATCCAGCGCATTGCCGATTACGCGTCGACGCTCGACGCGTTCAAGGCGGCCGAACCGGGCGTGCAGCCGGACGCCG
AGTGA

Upstream 100 bases:

>100_bases
CGCTCGCGCACGCACTGGCTGCGCACGGCGCTACACTCTGGTTCCCCGGCGGCGCGCCTGCGCATGCCTCGGCCGAATAC
GACATCAAGAGAGACGACCC

Downstream 100 bases:

>100_bases
CGCGCAGGCGCCGCACCGACGAAAAAGGCGCCCGAAGGCGCCTCGTGCGATGCGGTGCGGCGAAGGACGATCAGCCGAGC
AGCGCGTCCGCGAATTCCTC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSASYRVRIALNLKRLPYDYVPVHMLRDGGQQLKDEYRALNPDALVPTLVDGDATLQQSLAIIEYLDETHPE
PALLPKAPVDRAYVRAIALQIACEIHPLDNLRVLKYLKHTLKVSEDDKNAWYRHWIESGFASLETRLANDPRTGKLCFGD
TPTLADICLVPQVFNANRFSIDTTRYPTIQRIADYASTLDAFKAAEPGVQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSASYRVRIALNLKRLPYDYVPVHMLRDGGQQLKDEYRALNPDALVPTLVDGDATLQQSLAIIEYLDETHPE
PALLPKAPVDRAYVRAIALQIACEIHPLDNLRVLKYLKHTLKVSEDDKNAWYRHWIESGFASLETRLANDPRTGKLCFGD
TPTLADICLVPQVFNANRFSIDTTRYPTIQRIADYASTLDAFKAAEPGVQPDAE
>Mature_214_residues
MKLYSYFRSSASYRVRIALNLKRLPYDYVPVHMLRDGGQQLKDEYRALNPDALVPTLVDGDATLQQSLAIIEYLDETHPE
PALLPKAPVDRAYVRAIALQIACEIHPLDNLRVLKYLKHTLKVSEDDKNAWYRHWIESGFASLETRLANDPRTGKLCFGD
TPTLADICLVPQVFNANRFSIDTTRYPTIQRIADYASTLDAFKAAEPGVQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=50, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=38.5714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=47.7987421383648, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17551302, Length=210, Percent_Identity=43.3333333333333, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=42.654028436019, Blast_Score=156, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=41.9811320754717, Blast_Score=143, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=47.39336492891, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=47.39336492891, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=47.39336492891, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=46.6981132075472, Blast_Score=184, Evalue=5e-47,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24252; Mature: 24252

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSASYRVRIALNLKRLPYDYVPVHMLRDGGQQLKDEYRALNPDALVPTLVDG
CCHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEECC
DATLQQSLAIIEYLDETHPEPALLPKAPVDRAYVRAIALQIACEIHPLDNLRVLKYLKHT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVSEDDKNAWYRHWIESGFASLETRLANDPRTGKLCFGDTPTLADICLVPQVFNANRFS
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCEEE
IDTTRYPTIQRIADYASTLDAFKAAEPGVQPDAE
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSASYRVRIALNLKRLPYDYVPVHMLRDGGQQLKDEYRALNPDALVPTLVDG
CCHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEECC
DATLQQSLAIIEYLDETHPEPALLPKAPVDRAYVRAIALQIACEIHPLDNLRVLKYLKHT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVSEDDKNAWYRHWIESGFASLETRLANDPRTGKLCFGDTPTLADICLVPQVFNANRFS
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCEEE
IDTTRYPTIQRIADYASTLDAFKAAEPGVQPDAE
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]