Click here to switch to the map view.

The map label for this gene is dat [H]

Identifier: 161523592

GI number: 161523592

Start: 453311

End: 454237

Strand: Direct

Name: dat [H]

Synonym: Bmul_0412

Alternate gene names: 161523592

Gene position: 453311-454237 (Clockwise)

Preceding gene: 161523591

Following gene: 161523593

Centisome position: 13.15

GC content: 65.59

Gene sequence:

>927_bases
ATGAGCCCAACCGAATTCGAACCGATCGTCTATCTGAGCGTAGCGTCTCAAGAAGAAATGGTGCCGCTGTCGCAAGCGCG
CGTGCCGGTGCTCGATCGCGGATTCATCTTCGGCGACGGCGTGTATGAAGTCGTCCCCGTCTATGCGCACGACGGCGCGC
ACGTGCCGTTCCGGATCGCACAGCATCTGGAGCGGCTCGAGCGCAGCCTGAAGAAGATCGGCATCGACAATCCGCACGAC
GCGGCCGGCTGGCGCGCGCTGATCGAGCGCCTCGTCGACGCGAATGCGCAGGCGCTCGGCGACAGCCACGCGCTCGTGTA
CCTGCAGGTCACGCGCGGCGTCGCGAAGCGCGGCCATGCGTTTCCGGCCAACGCGGTGCCGACCGTATTCGCGATGACGA
GCCCGCTGCGGCTGCCGTCCGCGGAGGAGCGCGCGAAGGGTGTGCGCTGCGTGACGGCCGAAGATCGGCGCTGGCTGCAT
TGCGACATCAAGTCGACGTCGCTGCTCGGCAACGTGCTGATGGCGCAGCATGCGGCCGAGCGCGACGCGTTCGAAACGGT
GCAGCTGCGCGACGGCAATCTGACCGAAGGATCGTCGTCGAACGTCTGGGTCGTGAAGAACGGCGAGCTGTATGCGCCGC
CGCGCAGCAACAAGATCCTCGAAGGGATCCGCTACGCGCTGCTCGAGGAGCTCGCCGACGAATGCGGCATCCGCTTCGTT
GCGCGCGAGATCAGCGAAAGCGAACTGCGCGCGGCGGACGAAATCCTGATTACATCGGCGACGAAGGAAGTGCTGCCGGT
GACGTCGCTCGACGATCTGCCCGTACAGGGCGGCAAGCCGGGCCCGGTATTCGCGGCGTTGTACGACGCGTACCAGCGCG
CGAAAGCGCGCGAGTTTGAACAGTTTGACCTTTCCCGGAGGAAGTGA

Upstream 100 bases:

>100_bases
TTCGGCCGTATCTGGGACTCGATCCTGCTGATGTTCAGCAAGAAGAAGTAACGCGCGGCGGGTCTTTCTTTCGACGACTT
CATCCCCATCTGGTATAGCC

Downstream 100 bases:

>100_bases
TGAGCGAACCGACCAAAACCGTCGAGGTGACCGGCGCGATCGAAACGCCGAAGGAGTCGCTGCTGGAGTTCCCGTGCGAC
TTCCCGATCAAGATCATGGG

Product: class IV aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSPTEFEPIVYLSVASQEEMVPLSQARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIAQHLERLERSLKKIGIDNPHD
AAGWRALIERLVDANAQALGDSHALVYLQVTRGVAKRGHAFPANAVPTVFAMTSPLRLPSAEERAKGVRCVTAEDRRWLH
CDIKSTSLLGNVLMAQHAAERDAFETVQLRDGNLTEGSSSNVWVVKNGELYAPPRSNKILEGIRYALLEELADECGIRFV
AREISESELRAADEILITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLSRRK

Sequences:

>Translated_308_residues
MSPTEFEPIVYLSVASQEEMVPLSQARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIAQHLERLERSLKKIGIDNPHD
AAGWRALIERLVDANAQALGDSHALVYLQVTRGVAKRGHAFPANAVPTVFAMTSPLRLPSAEERAKGVRCVTAEDRRWLH
CDIKSTSLLGNVLMAQHAAERDAFETVQLRDGNLTEGSSSNVWVVKNGELYAPPRSNKILEGIRYALLEELADECGIRFV
AREISESELRAADEILITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLSRRK
>Mature_307_residues
SPTEFEPIVYLSVASQEEMVPLSQARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIAQHLERLERSLKKIGIDNPHDA
AGWRALIERLVDANAQALGDSHALVYLQVTRGVAKRGHAFPANAVPTVFAMTSPLRLPSAEERAKGVRCVTAEDRRWLHC
DIKSTSLLGNVLMAQHAAERDAFETVQLRDGNLTEGSSSNVWVVKNGELYAPPRSNKILEGIRYALLEELADECGIRFVA
REISESELRAADEILITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLSRRK

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=288, Percent_Identity=25.6944444444444, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 34070; Mature: 33939

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTEFEPIVYLSVASQEEMVPLSQARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIA
CCCCCCCCEEEEEECCCCCCCCHHHCCCCEEECCEEECCCCEEEEEEEECCCCCCHHHHH
QHLERLERSLKKIGIDNPHDAAGWRALIERLVDANAQALGDSHALVYLQVTRGVAKRGHA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEHHHHHHCCCC
FPANAVPTVFAMTSPLRLPSAEERAKGVRCVTAEDRRWLHCDIKSTSLLGNVLMAQHAAE
CCCCCCCCHHEECCCCCCCCHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHH
RDAFETVQLRDGNLTEGSSSNVWVVKNGELYAPPRSNKILEGIRYALLEELADECGIRFV
HCHHEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHH
AREISESELRAADEILITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFE
HHHHHHHHHHHHHHHEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFDLSRRK
HHHHCCCC
>Mature Secondary Structure 
SPTEFEPIVYLSVASQEEMVPLSQARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIA
CCCCCCCEEEEEECCCCCCCCHHHCCCCEEECCEEECCCCEEEEEEEECCCCCCHHHHH
QHLERLERSLKKIGIDNPHDAAGWRALIERLVDANAQALGDSHALVYLQVTRGVAKRGHA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEHHHHHHCCCC
FPANAVPTVFAMTSPLRLPSAEERAKGVRCVTAEDRRWLHCDIKSTSLLGNVLMAQHAAE
CCCCCCCCHHEECCCCCCCCHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHH
RDAFETVQLRDGNLTEGSSSNVWVVKNGELYAPPRSNKILEGIRYALLEELADECGIRFV
HCHHEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHH
AREISESELRAADEILITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFE
HHHHHHHHHHHHHHHEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFDLSRRK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]