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The map label for this gene is dacA [H]

Identifier: 161523591

GI number: 161523591

Start: 451945

End: 453261

Strand: Direct

Name: dacA [H]

Synonym: Bmul_0411

Alternate gene names: 161523591

Gene position: 451945-453261 (Clockwise)

Preceding gene: 161523589

Following gene: 161523592

Centisome position: 13.11

GC content: 67.2

Gene sequence:

>1317_bases
ATGCGTCTGTCTTCCCAAGGCCTCAAGTCCCTCGCTTCTTCCATCGCCTTCGGCACCGCCGCGCGCAACGTCGCGCTCGG
CGTGATGCTGCCCGTCGCGCTCGCGTCGACGATCGTCGTCGCCGAGGCGAAGCCGGCCGCCAAGGCCAAGGCCGCGCATG
CGGCGCCGGCCGCCGCCGAGCAGTCGACCGGCGCACCGGCGACCTTCATGCCGGGCGCAGTGCCGCCGCCGGGCGTGAAC
GCGCGCTCGTGGGTGCTCGTCGACGCGACGAGCAACACGGTGCTCGCGTCGGGCAATGCGGACGAGCGTGTGGAGCCGGC
GTCGCTGACGAAGCTGATGACCGCGTACCTCGTGTTCGAGGCGCTCGACAAGAAGAAGATCTCGATGGAGCAGATCGTCA
CGCCGAGCGAGGCCGTGCGCCGTGTCGGCCGCGACGAGTCGCGGATGTTCATCGAGGCGAACAAGCCGGTGTCGGTGCAC
GACCTCGTGTACGGGATGATCATCCAGTCGGGCAATGACGCGGCGATCGCGCTGGCCGAACTGGTCGGCGGCAGCGAAGC
GCAGTTCGTCACGATGATGAACGACGAGGCGCAGCGCCTCGGCATGAAGGGCACGCACTTCGCCGACGTGAACGGCATGC
CCGACCCGAACCACTACACGACGGCCGGCGACCTCGCGAAGCTGTCCGCGCACCTGATCCGCGATTTTCCGCAGTACTAC
GGCATCTTCTCGGAGAAGGAATTCAAGTACAACAACATCCGCCAGGGCAACCGCAACCGTCTGCTGTGGCTCGATCCGAC
CGTCGACGGCCTGAAGACCGGCCACACGCAGGCGGCCGGCTTCTGCCTGATCTCGTCGGCGAAGCGGCCGATTCCGGGCG
CTTCGGACGCGCAGCGCCGTCTGGTCGCCGTGATGATGGGCGAGCCGAAGGAGAGCGACCGCACGCAGGACAGCATGAAG
ATGCTGAACTACGGCTATAGCGCGTTCGACGCGGTGCGCCTGTTCAAGGGGGGCCAGGCGATCTCGACGCCGCGCCTCTA
CAAGGGCAAGGAGAACACCGTGCAGGTCGGCGTCGCGAGCGACCAGTGGGTGACCGTGCCGCGCGGCCTCGGCGACAAGG
TCAAGCCGGAAGTCGACGTCAACGCGCCGCTGATCGCGCCGCTCGCGCAAGGGCAGCAGGTCGGCACCGTGAAGGTCGTC
GCCGACGGCAAGACGCTGTCCGAATTCCCGGTCGTCGCGCTGCAGGCCGTGCCGGAAGCGGGTATCTTCGGCCGTATCTG
GGACTCGATCCTGCTGATGTTCAGCAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
GTCGTCCGAGGTGTCGGGCGAGTGCCCGAAACGCCTGCCGCCTTGCCGTTCAACCGTGCGCGTTTCGCGCCGCACTTTCT
GCCATCAGCCTGAATCGATC

Downstream 100 bases:

>100_bases
CGCGCGGCGGGTCTTTCTTTCGACGACTTCATCCCCATCTGGTATAGCCATGAGCCCAACCGAATTCGAACCGATCGTCT
ATCTGAGCGTAGCGTCTCAA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRLSSQGLKSLASSIAFGTAARNVALGVMLPVALASTIVVAEAKPAAKAKAAHAAPAAAEQSTGAPATFMPGAVPPPGVN
ARSWVLVDATSNTVLASGNADERVEPASLTKLMTAYLVFEALDKKKISMEQIVTPSEAVRRVGRDESRMFIEANKPVSVH
DLVYGMIIQSGNDAAIALAELVGGSEAQFVTMMNDEAQRLGMKGTHFADVNGMPDPNHYTTAGDLAKLSAHLIRDFPQYY
GIFSEKEFKYNNIRQGNRNRLLWLDPTVDGLKTGHTQAAGFCLISSAKRPIPGASDAQRRLVAVMMGEPKESDRTQDSMK
MLNYGYSAFDAVRLFKGGQAISTPRLYKGKENTVQVGVASDQWVTVPRGLGDKVKPEVDVNAPLIAPLAQGQQVGTVKVV
ADGKTLSEFPVVALQAVPEAGIFGRIWDSILLMFSKKK

Sequences:

>Translated_438_residues
MRLSSQGLKSLASSIAFGTAARNVALGVMLPVALASTIVVAEAKPAAKAKAAHAAPAAAEQSTGAPATFMPGAVPPPGVN
ARSWVLVDATSNTVLASGNADERVEPASLTKLMTAYLVFEALDKKKISMEQIVTPSEAVRRVGRDESRMFIEANKPVSVH
DLVYGMIIQSGNDAAIALAELVGGSEAQFVTMMNDEAQRLGMKGTHFADVNGMPDPNHYTTAGDLAKLSAHLIRDFPQYY
GIFSEKEFKYNNIRQGNRNRLLWLDPTVDGLKTGHTQAAGFCLISSAKRPIPGASDAQRRLVAVMMGEPKESDRTQDSMK
MLNYGYSAFDAVRLFKGGQAISTPRLYKGKENTVQVGVASDQWVTVPRGLGDKVKPEVDVNAPLIAPLAQGQQVGTVKVV
ADGKTLSEFPVVALQAVPEAGIFGRIWDSILLMFSKKK
>Mature_438_residues
MRLSSQGLKSLASSIAFGTAARNVALGVMLPVALASTIVVAEAKPAAKAKAAHAAPAAAEQSTGAPATFMPGAVPPPGVN
ARSWVLVDATSNTVLASGNADERVEPASLTKLMTAYLVFEALDKKKISMEQIVTPSEAVRRVGRDESRMFIEANKPVSVH
DLVYGMIIQSGNDAAIALAELVGGSEAQFVTMMNDEAQRLGMKGTHFADVNGMPDPNHYTTAGDLAKLSAHLIRDFPQYY
GIFSEKEFKYNNIRQGNRNRLLWLDPTVDGLKTGHTQAAGFCLISSAKRPIPGASDAQRRLVAVMMGEPKESDRTQDSMK
MLNYGYSAFDAVRLFKGGQAISTPRLYKGKENTVQVGVASDQWVTVPRGLGDKVKPEVDVNAPLIAPLAQGQQVGTVKVV
ADGKTLSEFPVVALQAVPEAGIFGRIWDSILLMFSKKK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=369, Percent_Identity=41.4634146341463, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=38.8451443569554, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1786851, Length=373, Percent_Identity=39.4101876675603, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082054, Length=221, Percent_Identity=31.2217194570136, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46798; Mature: 46798

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSSQGLKSLASSIAFGTAARNVALGVMLPVALASTIVVAEAKPAAKAKAAHAAPAAAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCC
QSTGAPATFMPGAVPPPGVNARSWVLVDATSNTVLASGNADERVEPASLTKLMTAYLVFE
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
ALDKKKISMEQIVTPSEAVRRVGRDESRMFIEANKPVSVHDLVYGMIIQSGNDAAIALAE
HHCCHHCCHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHEEHEEECCCCHHHHHHH
LVGGSEAQFVTMMNDEAQRLGMKGTHFADVNGMPDPNHYTTAGDLAKLSAHLIRDFPQYY
HHCCCCCEEEEEECCHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GIFSEKEFKYNNIRQGNRNRLLWLDPTVDGLKTGHTQAAGFCLISSAKRPIPGASDAQRR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCHHCEEEEECCCCCCCCCCHHHHH
LVAVMMGEPKESDRTQDSMKMLNYGYSAFDAVRLFKGGQAISTPRLYKGKENTVQVGVAS
EEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
DQWVTVPRGLGDKVKPEVDVNAPLIAPLAQGQQVGTVKVVADGKTLSEFPVVALQAVPEA
CCEEECCCCCCCCCCCCCCCCCCEECHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC
GIFGRIWDSILLMFSKKK
CHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLSSQGLKSLASSIAFGTAARNVALGVMLPVALASTIVVAEAKPAAKAKAAHAAPAAAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCC
QSTGAPATFMPGAVPPPGVNARSWVLVDATSNTVLASGNADERVEPASLTKLMTAYLVFE
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
ALDKKKISMEQIVTPSEAVRRVGRDESRMFIEANKPVSVHDLVYGMIIQSGNDAAIALAE
HHCCHHCCHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHEEHEEECCCCHHHHHHH
LVGGSEAQFVTMMNDEAQRLGMKGTHFADVNGMPDPNHYTTAGDLAKLSAHLIRDFPQYY
HHCCCCCEEEEEECCHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GIFSEKEFKYNNIRQGNRNRLLWLDPTVDGLKTGHTQAAGFCLISSAKRPIPGASDAQRR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCHHCEEEEECCCCCCCCCCHHHHH
LVAVMMGEPKESDRTQDSMKMLNYGYSAFDAVRLFKGGQAISTPRLYKGKENTVQVGVAS
EEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
DQWVTVPRGLGDKVKPEVDVNAPLIAPLAQGQQVGTVKVVADGKTLSEFPVVALQAVPEA
CCEEECCCCCCCCCCCCCCCCCCEECHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC
GIFGRIWDSILLMFSKKK
CHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]