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The map label for this gene is aroK [H]

Identifier: 161523475

GI number: 161523475

Start: 322969

End: 323457

Strand: Direct

Name: aroK [H]

Synonym: Bmul_0295

Alternate gene names: 161523475

Gene position: 322969-323457 (Clockwise)

Preceding gene: 161523474

Following gene: 161523476

Centisome position: 9.37

GC content: 65.85

Gene sequence:

>489_bases
GTGGGCCGTGCGGTCGCGCGCCGGCTCGACAGGACGTTCTTCGACTCCGACCACGAAATCGAGGCCCGCACGGGCGCGCG
CATTCCGGTGATCTTCGAGCTGGAGGGCGAGGCCGGGTTTCGCGACCGCGAAGCGCAGATGATCGCCGAGCTCACGCAGC
GCGAGAACATCGTGCTCGCGACGGGCGGCGGCGCGGTGCTGCGGGCCGAGAATCGCGCATGCCTGAAAGGCAACGGCATC
GTCGTCTACCTGCGCGCCAATCCGCACGATCTGTGGCTTCGCACACGCAAGGACAAAAATCGTCCGCTGCTGCAGACCGA
CGATCCGAAGGCCCGTCTCGAAGCGCTCTACGAAGTGCGCGACCCGTTGTACCGCGAATGCGCGGACTTCGTGATCGAAA
CGGGCCGCCCGTCCGTGAACGGTCTCGTCAACATGGTGCTGATGCAACTCGAACTGGCGGGCGTCGTCGCCAAGCCGCTA
CAAGCATGA

Upstream 100 bases:

>100_bases
CCACACACCAGCAAGATTGAAGCAGAGGAAGCCGTTGCAAGCGCGGGACCCACATGCAAACGTATTTTTCGTCGGCCTTA
TGGGAGCGGGTAAGACCACC

Downstream 100 bases:

>100_bases
TTACTGTCAACGTCGATCTGGGCGACCGCGCCTACCCGATTCACATTGGCGCCGGCCTGATCGGCCGCGCCGAGCTGTTC
GCTCCCCACATCAAAGGTTC

Product: shikimate kinase

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGRAVARRLDRTFFDSDHEIEARTGARIPVIFELEGEAGFRDREAQMIAELTQRENIVLATGGGAVLRAENRACLKGNGI
VVYLRANPHDLWLRTRKDKNRPLLQTDDPKARLEALYEVRDPLYRECADFVIETGRPSVNGLVNMVLMQLELAGVVAKPL
QA

Sequences:

>Translated_162_residues
MGRAVARRLDRTFFDSDHEIEARTGARIPVIFELEGEAGFRDREAQMIAELTQRENIVLATGGGAVLRAENRACLKGNGI
VVYLRANPHDLWLRTRKDKNRPLLQTDDPKARLEALYEVRDPLYRECADFVIETGRPSVNGLVNMVLMQLELAGVVAKPL
QA
>Mature_161_residues
GRAVARRLDRTFFDSDHEIEARTGARIPVIFELEGEAGFRDREAQMIAELTQRENIVLATGGGAVLRAENRACLKGNGIV
VYLRANPHDLWLRTRKDKNRPLLQTDDPKARLEALYEVRDPLYRECADFVIETGRPSVNGLVNMVLMQLELAGVVAKPLQ
A

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=152, Percent_Identity=48.0263157894737, Blast_Score=138, Evalue=1e-34,
Organism=Escherichia coli, GI1786587, Length=142, Percent_Identity=36.6197183098592, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 18198; Mature: 18067

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAVARRLDRTFFDSDHEIEARTGARIPVIFELEGEAGFRDREAQMIAELTQRENIVLA
CCHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
TGGGAVLRAENRACLKGNGIVVYLRANPHDLWLRTRKDKNRPLLQTDDPKARLEALYEVR
ECCCEEEEECCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
DPLYRECADFVIETGRPSVNGLVNMVLMQLELAGVVAKPLQA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRAVARRLDRTFFDSDHEIEARTGARIPVIFELEGEAGFRDREAQMIAELTQRENIVLA
CHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
TGGGAVLRAENRACLKGNGIVVYLRANPHDLWLRTRKDKNRPLLQTDDPKARLEALYEVR
ECCCEEEEECCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
DPLYRECADFVIETGRPSVNGLVNMVLMQLELAGVVAKPLQA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA