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The map label for this gene is pilQ [H]

Identifier: 161523474

GI number: 161523474

Start: 321141

End: 322862

Strand: Direct

Name: pilQ [H]

Synonym: Bmul_0294

Alternate gene names: 161523474

Gene position: 321141-322862 (Clockwise)

Preceding gene: 161523473

Following gene: 161523475

Centisome position: 9.31

GC content: 72.71

Gene sequence:

>1722_bases
GTGACGAAGCTCAACCGGTACGGCATCGCGGTCTGGTTCGGCATCGGAGTCGGCGCCGTCGGCCTCGGCCTCGGCGCGGC
TACGGCGAACGCCGCGTTGCCGCCGCTGCCTGCGGCCTGGCCGGTCGCCTCGACGATGGCTGAAGCGCGCGAGGCGCCGC
TGCCGCCCGGCGTGCCGGACGGCGCGATCGATGACGCAATCGACGGTGCGGCCGGTCGCGCGGCGTCCTACCCCCAGACC
GACCGCACGCACGAAGCCGCGTCGCCACCCGCGCCCCCGCCGCCGTTCGAGCACGCATCCCGCGTCGCGCAGCACGCGCA
ACCGGCCGCGCCGGCACCGGCGCTGGAAGGGCCGCCGATCCCGCTGCCGCCGCCCGCGCGCATGAATGACGCGGCCGCGC
ATCGCGCAGGCGACGCGGACGACACGCGCCGCATCTCGTTGAACCTGCAGCGCGCGGACCTGACGGCCGCCTTCGACGCA
TTCGCACGCTTCACGGGCCTGAACATCGTCGTGAGCGAGCAGGTTCGCGGCACCGTGACCTTGCGTCTGAACAATGTCCG
TTGGCGCGATGCATTCGACACGCTGCTCGACACGCATGGGCTCGCGATGTCCCGGCGCGGCGACATCGTCTGGGTGACGC
GGGCCGCCGAACTGGCGGTGCGCGAGCGCGAGCGCTTCGAAACGCACGCGCGTGCCGCCGATCTGGAGCCGCTCGAGAGC
CGCCGGTTCGAATTGCACTATGCGCGCGCGCAGGACGTGCAGCGGCTGCTGGCCGGCGCGACCGGCCAGCGCCTGCTGTC
GAAGCGCGGTGCCGCGGCCGCCGATGCGCGCACGAACCTGCTGTTCGTCACCGATCTCGCGCCGCGCATCGCGCAGATCG
CCGATCTGATCGCGGCCCTCGACCGGCCGTCGCGGCAGGTGCGCATCGAGGCGCGCATCGTCGAGGGCGAGCAGGGCTTT
TCGCGTCATCTCGGCGCCCGGCTCGCGTTGCGCGCGCAAGGGCGGCCGGCGGCGGCCGAAGCTGGCGCGTCCCCGGCCGG
CGCCCGCACGGCGCTCGATCTGGCCGCGCGGCCGCTCGCCGGCTTCGATGCCGCGACGGCCGGCTTCACGCTGCTCGCCG
CGCCGCTCAGCCGCGTGCTCGACGTCGAGCTGAGCGCGCTGGAGGCGCAGGGGCGCGGCCAGATCGTGTCGAGCCCGCGC
GTCGTGACGGCCGATCGCGCGAAGGCGCTCGTCGAGCAGGGCGCGGAGCTGCCGTATCAGGCAAAGGTCGGCAACGGCGT
GACGGGCGTGCAGTTCCGCCGCGCGACGCTGAAGCTCGAGGTCGAGCCGCAGATCACGCCGGACGGGCGCGTCGTGCTCG
ACCTCGATGTGACGAAGGACAGCGTCGGCGAACCGACCGCGGCCGGCCCCGCAATCCATACGAAACACGTGCAAACGCGC
GTCGAAGTCGACGACGGCGGAACGGTCGCGATCGGCGGGATCTATGAGCGGCTGCATCGGCACGATGTGACGCGGGTGCC
GCTGTTGGGCAAAATACCGGTTCTGGGCGCGCTTTTCCGGCATCGCGCGCAGCGCGAGCAGCGTAGCGAGCTGGTCGTGT
TCATCACGCCGACGATCGTCAGCGCCGGCTGCGGCCCGCCCGGCACAGCCGACGAAAATGCCGAGAAATCGGGGCCGGAA
GGGGGTGGCGCAGGCTCGACAAGGCAGTCGCTTTGCCAGTAA

Upstream 100 bases:

>100_bases
CGCTCGGTGCGTTGCGCGTTGCGCGCGTCGATGCGGGCGCCGTGACGCTGTCGACGTCGGACGGCCCGCGGCGGCTCGCA
CTCGAAGACGGAGGCCGCTC

Downstream 100 bases:

>100_bases
GCTGCGCCACACACCAGCAAGATTGAAGCAGAGGAAGCCGTTGCAAGCGCGGGACCCACATGCAAACGTATTTTTCGTCG
GCCTTATGGGAGCGGGTAAG

Product: type IV pilus secretin PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTKLNRYGIAVWFGIGVGAVGLGLGAATANAALPPLPAAWPVASTMAEAREAPLPPGVPDGAIDDAIDGAAGRAASYPQT
DRTHEAASPPAPPPPFEHASRVAQHAQPAAPAPALEGPPIPLPPPARMNDAAAHRAGDADDTRRISLNLQRADLTAAFDA
FARFTGLNIVVSEQVRGTVTLRLNNVRWRDAFDTLLDTHGLAMSRRGDIVWVTRAAELAVRERERFETHARAADLEPLES
RRFELHYARAQDVQRLLAGATGQRLLSKRGAAAADARTNLLFVTDLAPRIAQIADLIAALDRPSRQVRIEARIVEGEQGF
SRHLGARLALRAQGRPAAAEAGASPAGARTALDLAARPLAGFDAATAGFTLLAAPLSRVLDVELSALEAQGRGQIVSSPR
VVTADRAKALVEQGAELPYQAKVGNGVTGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSVGEPTAAGPAIHTKHVQTR
VEVDDGGTVAIGGIYERLHRHDVTRVPLLGKIPVLGALFRHRAQREQRSELVVFITPTIVSAGCGPPGTADENAEKSGPE
GGGAGSTRQSLCQ

Sequences:

>Translated_573_residues
MTKLNRYGIAVWFGIGVGAVGLGLGAATANAALPPLPAAWPVASTMAEAREAPLPPGVPDGAIDDAIDGAAGRAASYPQT
DRTHEAASPPAPPPPFEHASRVAQHAQPAAPAPALEGPPIPLPPPARMNDAAAHRAGDADDTRRISLNLQRADLTAAFDA
FARFTGLNIVVSEQVRGTVTLRLNNVRWRDAFDTLLDTHGLAMSRRGDIVWVTRAAELAVRERERFETHARAADLEPLES
RRFELHYARAQDVQRLLAGATGQRLLSKRGAAAADARTNLLFVTDLAPRIAQIADLIAALDRPSRQVRIEARIVEGEQGF
SRHLGARLALRAQGRPAAAEAGASPAGARTALDLAARPLAGFDAATAGFTLLAAPLSRVLDVELSALEAQGRGQIVSSPR
VVTADRAKALVEQGAELPYQAKVGNGVTGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSVGEPTAAGPAIHTKHVQTR
VEVDDGGTVAIGGIYERLHRHDVTRVPLLGKIPVLGALFRHRAQREQRSELVVFITPTIVSAGCGPPGTADENAEKSGPE
GGGAGSTRQSLCQ
>Mature_572_residues
TKLNRYGIAVWFGIGVGAVGLGLGAATANAALPPLPAAWPVASTMAEAREAPLPPGVPDGAIDDAIDGAAGRAASYPQTD
RTHEAASPPAPPPPFEHASRVAQHAQPAAPAPALEGPPIPLPPPARMNDAAAHRAGDADDTRRISLNLQRADLTAAFDAF
ARFTGLNIVVSEQVRGTVTLRLNNVRWRDAFDTLLDTHGLAMSRRGDIVWVTRAAELAVRERERFETHARAADLEPLESR
RFELHYARAQDVQRLLAGATGQRLLSKRGAAAADARTNLLFVTDLAPRIAQIADLIAALDRPSRQVRIEARIVEGEQGFS
RHLGARLALRAQGRPAAAEAGASPAGARTALDLAARPLAGFDAATAGFTLLAAPLSRVLDVELSALEAQGRGQIVSSPRV
VTADRAKALVEQGAELPYQAKVGNGVTGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSVGEPTAAGPAIHTKHVQTRV
EVDDGGTVAIGGIYERLHRHDVTRVPLLGKIPVLGALFRHRAQREQRSELVVFITPTIVSAGCGPPGTADENAEKSGPEG
GGAGSTRQSLCQ

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=410, Percent_Identity=34.8780487804878, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI87082242, Length=356, Percent_Identity=28.0898876404494, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 60400; Mature: 60269

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLNRYGIAVWFGIGVGAVGLGLGAATANAALPPLPAAWPVASTMAEAREAPLPPGVPD
CCCCCCCCEEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
GAIDDAIDGAAGRAASYPQTDRTHEAASPPAPPPPFEHASRVAQHAQPAAPAPALEGPPI
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PLPPPARMNDAAAHRAGDADDTRRISLNLQRADLTAAFDAFARFTGLNIVVSEQVRGTVT
CCCCCCCCCCHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCEEEE
LRLNNVRWRDAFDTLLDTHGLAMSRRGDIVWVTRAAELAVRERERFETHARAADLEPLES
EEECCEEHHHHHHHHHHHCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHC
RRFELHYARAQDVQRLLAGATGQRLLSKRGAAAADARTNLLFVTDLAPRIAQIADLIAAL
CCEEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
DRPSRQVRIEARIVEGEQGFSRHLGARLALRAQGRPAAAEAGASPAGARTALDLAARPLA
CCCCCEEEEEEEEECCCCCHHHHHCCEEEEEECCCCCHHHCCCCCCCCHHHHHHHHCCCC
GFDAATAGFTLLAAPLSRVLDVELSALEAQGRGQIVSSPRVVTADRAKALVEQGAELPYQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEHHHHHHHHHCCCCCCEE
AKVGNGVTGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSVGEPTAAGPAIHTKHVQTR
EECCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
VEVDDGGTVAIGGIYERLHRHDVTRVPLLGKIPVLGALFRHRAQREQRSELVVFITPTIV
EEECCCCEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHH
SAGCGPPGTADENAEKSGPEGGGAGSTRQSLCQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
TKLNRYGIAVWFGIGVGAVGLGLGAATANAALPPLPAAWPVASTMAEAREAPLPPGVPD
CCCCCCCEEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
GAIDDAIDGAAGRAASYPQTDRTHEAASPPAPPPPFEHASRVAQHAQPAAPAPALEGPPI
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PLPPPARMNDAAAHRAGDADDTRRISLNLQRADLTAAFDAFARFTGLNIVVSEQVRGTVT
CCCCCCCCCCHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCEEEE
LRLNNVRWRDAFDTLLDTHGLAMSRRGDIVWVTRAAELAVRERERFETHARAADLEPLES
EEECCEEHHHHHHHHHHHCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHC
RRFELHYARAQDVQRLLAGATGQRLLSKRGAAAADARTNLLFVTDLAPRIAQIADLIAAL
CCEEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
DRPSRQVRIEARIVEGEQGFSRHLGARLALRAQGRPAAAEAGASPAGARTALDLAARPLA
CCCCCEEEEEEEEECCCCCHHHHHCCEEEEEECCCCCHHHCCCCCCCCHHHHHHHHCCCC
GFDAATAGFTLLAAPLSRVLDVELSALEAQGRGQIVSSPRVVTADRAKALVEQGAELPYQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEHHHHHHHHHCCCCCCEE
AKVGNGVTGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSVGEPTAAGPAIHTKHVQTR
EECCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
VEVDDGGTVAIGGIYERLHRHDVTRVPLLGKIPVLGALFRHRAQREQRSELVVFITPTIV
EEECCCCEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHH
SAGCGPPGTADENAEKSGPEGGGAGSTRQSLCQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]