| Definition | Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010084 |
| Length | 3,448,466 |
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The map label for this gene is mrcA [H]
Identifier: 161523470
GI number: 161523470
Start: 316135
End: 318528
Strand: Reverse
Name: mrcA [H]
Synonym: Bmul_0290
Alternate gene names: 161523470
Gene position: 318528-316135 (Counterclockwise)
Preceding gene: 161523483
Following gene: 161523469
Centisome position: 9.24
GC content: 65.54
Gene sequence:
>2394_bases ATGCCTATGCAATCCACGTCTCCTACGCCCCCGCCTCCATCCCCGGAGCCGAAGAAGCGCCCCTGGTGGCAAAAAGCGCT GTTCGGCTTCGCCGCGATGTGCGTGGCGCTCGTCGTGGCCGGCGGCCTCGTCCTCGGCTATGCGCTCGTCGTCGCATGGC CGAACATGCCGTCGCTCGACGCGCTGACCGACTATCGGCCGAAGGTGCCGCTGCGCATCTATACGTCCGATCACGTGCTG ATCGGCGAATTCGGCGAGGAACGCCGCGACATCGTCCATTTCAAGGACGTCCCCGATTCGCTGAAAAAGGCGATCCTCGC GATCGAGGATGCGCGCTTCTACGATCACGGCGGCGTCGACCTCACCGGCATCCTCCGCGCAGGCGTCGTCGCGCTGACGA ACGGCCACGCGACACAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAGCGAGAAGACGTAC ACGCGCAAGATCTACGAGATGCTGCTCGCGTACCGGATCGAGCGCGCGCTGACGAAAGATCAGATTCTCGAGGTCTATAT GAATCAGATCTATCTCGGCCAGCGCGCGTACGGCTTCGCGAGTGCCGCGCGCGTCTATTTCGGCAAGGACCTGAAGGACA TCACACTCGCCGAAGCGGCGATGCTGGCGGGGCTGCCGAAGGCGCCGTCCGCGTACAACCCGGTCGTCAATCCGAAGCGC GCGAAGGTGCGCCAGGAGTACATCCTGCAGCGGATGCTCGAGCTGAACTTCATCACGCGCGAGCAGTACGACGAAGCCGT CGCGCAGCCGCTCGTCGTCAAGGGCGCGGGCCGCGAGTACAGCGTGCATGCCGAATACGTCGCGGAAATGGTGCGTCAGA TGATGTATGCGCAATACCGCGAGGAAACCTACACGCGCGGCTTCAACGTCATCACGACGATCGATTCCGCCGATCAGCAG GTTGCGTATACCGCGCTGCGCAAGGGCATCATGGATTACGAGCGGCGCCACGGCTATCGCGGGCCGGAAGGCTTCATCGA GCTGCCTTCGGGCGCGGACGAGCGCGAACAGGCCATCGACGACGCGCTGCTCGAGCACCCGGACAACGGCGAGCTGATCG CGGCCGTCGTCACGGCCGCGAGCCCGCGCCAGATCACCGTCGCGTTCATCGACGGCAGCAGCGCGACGATCGAAGGCGAG AACCTGCGCTTCGCGGCCGGCGCGCTGTCGGCGAACGCGCAGCCGAACCGTCGCATTCGTCCGGGCGCAATCGTTCGCGT CGTGAAAAACGACGCGGGCAAATGGTCGATCACGCAGTTGCCGCAGGTCGAAGGCGCGTTCATCTCGATCGTGCCGCAGG ACGGCGCGATCCGCTCGCTGGTCGGCGGCTTCGACTACAACAAGAACAAGTTCAACCACGTGACGCAGGCGTGGCGGCAA CCCGGTTCGTCGTTCAAGCCGTTCATCTACTCGGCATCGCTCGACAAGGGGCTCGGGCCGGCCACGGTCATCAACGACGG GCCGCTCTATTTCAGCGCGGCGGAAACCGGCGGCCAGCCCTGGGAACCGAAGAACTACGGCGGCGGCTTCGAAGGGCCGA TGTCGATGCGCACCGCGCTGCAGCGCTCGCGCAACCTCGTGTCGATCCGGATCCTCAACCACATCGGCACGAAGTACGCG CAGCAGTACATCACGCGCTTCGGCTTCGACGCCGATCGCCATCCGGCCTATCTGCCGATGGCGCTCGGCGCCGGCCTCGT CACGCCGCTGCAGATGGCCGGCGCGTATTCGGTGTTCGCGAACGGCGGCTATCGCGTGAACCCGTATCTGATCGCCGAAG TCACCGATCCGAACGGTGCGATCGTCGCGCGCGCACAACCGCTCATCGCCGAGCAGAATGCGCCGCGCGCGATCGATGCG CGCAACGCGTACGTGATGAACAGCCTGTTGCAGAGCGTCGCGCAGCGCGGTACCGGCGCGAAGACCAACGTGCTCAAGCG CACGGATCTCGCGGGCAAGACCGGCACGACCAACGATTCGCACGATGCGTGGTTCGCCGGCTATCAGCACACGCTCGCCG CGATCGCATGGATCGGCTACGACAATCCGCGCAGCCTCGGCGATCGCGAAACCGGCGGCGGCCTCGCGCTGCCGGTATGG ATCGACTACATGGGTGCGGCGCTCAAGGGCGTGCCCGACTTCAAGCCGACGATGCCCGACGGCGTCGAATCGCTCGGCGG CGAGCTGTATTTCACCGACTTCACGCCCGGCCACGGCTTCGTGTCGACCGTCGGCGTGCCGCAGGCGCCCGCGTCGCAAG AGGCCGACGATGCCGTGCCGCATGTCGACGAGCAGGAAAAGCAGGACATCATGAACCTGTTCCGCGGCCACTGA
Upstream 100 bases:
>100_bases GCTATGCTGATCTCGGCACGGTCGGGCGGTTGCCCGGCCGCTCCCCCCGCGCCGGGGCCGCCTCCGGCGGGCAGCTATAA TCGCGGGACTGTTTTCCGGT
Downstream 100 bases:
>100_bases TCGGCGGCCCCGGCATGAGAAAAGCGCCCCTGAACTGCACCCCAAAAGTTGGATACCGATCCAACCTTTGGGGTGTTTTT CATGACGAAGTACGACGAGC
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 797; Mature: 796
Protein sequence:
>797_residues MPMQSTSPTPPPPSPEPKKRPWWQKALFGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDIVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGILRAGVVALTNGHATQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFITREQYDEAVAQPLVVKGAGREYSVHAEYVAEMVRQMMYAQYREETYTRGFNVITTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPSGADEREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSSATIEGE NLRFAAGALSANAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFISIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYA QQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLIAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGAKTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVW IDYMGAALKGVPDFKPTMPDGVESLGGELYFTDFTPGHGFVSTVGVPQAPASQEADDAVPHVDEQEKQDIMNLFRGH
Sequences:
>Translated_797_residues MPMQSTSPTPPPPSPEPKKRPWWQKALFGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDIVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGILRAGVVALTNGHATQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFITREQYDEAVAQPLVVKGAGREYSVHAEYVAEMVRQMMYAQYREETYTRGFNVITTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPSGADEREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSSATIEGE NLRFAAGALSANAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFISIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYA QQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLIAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGAKTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVW IDYMGAALKGVPDFKPTMPDGVESLGGELYFTDFTPGHGFVSTVGVPQAPASQEADDAVPHVDEQEKQDIMNLFRGH >Mature_796_residues PMQSTSPTPPPPSPEPKKRPWWQKALFGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVLI GEFGEERRDIVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGILRAGVVALTNGHATQGASTITMQVARNFFLSSEKTYT RKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRA KVRQEYILQRMLELNFITREQYDEAVAQPLVVKGAGREYSVHAEYVAEMVRQMMYAQYREETYTRGFNVITTIDSADQQV AYTALRKGIMDYERRHGYRGPEGFIELPSGADEREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSSATIEGEN LRFAAGALSANAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFISIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQP GSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYAQ QYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLIAEQNAPRAIDAR NAYVMNSLLQSVAQRGTGAKTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWI DYMGAALKGVPDFKPTMPDGVESLGGELYFTDFTPGHGFVSTVGVPQAPASQEADDAVPHVDEQEKQDIMNLFRGH
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=609, Percent_Identity=42.8571428571429, Blast_Score=496, Evalue=1e-141, Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=38.5542168674699, Blast_Score=151, Evalue=2e-37, Organism=Escherichia coli, GI1788867, Length=229, Percent_Identity=32.7510917030568, Blast_Score=115, Evalue=1e-26, Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=32.5842696629214, Blast_Score=92, Evalue=1e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 87345; Mature: 87214
Theoretical pI: Translated: 7.09; Mature: 7.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPMQSTSPTPPPPSPEPKKRPWWQKALFGFAAMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDIVHFKDVPDSLKKAILAIEDARFYDHGGVD HHHCCCCCCCEEEECCCEEEEECCCCCHHHEEEECCCCHHHHHHHHHHHCCCEECCCCCC LTGILRAGVVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFITREQYDEAVAQPLVVKGAGREYSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECHHHHHHHHHHHHHHHHH EETYTRGFNVITTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPSGADEREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSSATIEGENLRFAAGALSANAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCCCC PGAIVRVVKNDAGKWSITQLPQVEGAFISIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNHIGTKYAQQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAYSVFA HHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHCCEEEEE NGGYRVNPYLIAEVTDPNGAIVARAQPLIAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC KTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVW CHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEHHHH IDYMGAALKGVPDFKPTMPDGVESLGGELYFTDFTPGHGFVSTVGVPQAPASQEADDAVP HHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCC HVDEQEKQDIMNLFRGH CCCCHHHHHHHHHHCCC >Mature Secondary Structure PMQSTSPTPPPPSPEPKKRPWWQKALFGFAAMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDIVHFKDVPDSLKKAILAIEDARFYDHGGVD HHHCCCCCCCEEEECCCEEEEECCCCCHHHEEEECCCCHHHHHHHHHHHCCCEECCCCCC LTGILRAGVVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFITREQYDEAVAQPLVVKGAGREYSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECHHHHHHHHHHHHHHHHH EETYTRGFNVITTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPSGADEREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSSATIEGENLRFAAGALSANAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCCCC PGAIVRVVKNDAGKWSITQLPQVEGAFISIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNHIGTKYAQQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAYSVFA HHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHCCEEEEE NGGYRVNPYLIAEVTDPNGAIVARAQPLIAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC KTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVW CHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEHHHH IDYMGAALKGVPDFKPTMPDGVESLGGELYFTDFTPGHGFVSTVGVPQAPASQEADDAVP HHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCC HVDEQEKQDIMNLFRGH CCCCHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA