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The map label for this gene is rep [H]

Identifier: 161523326

GI number: 161523326

Start: 163516

End: 165681

Strand: Direct

Name: rep [H]

Synonym: Bmul_0146

Alternate gene names: 161523326

Gene position: 163516-165681 (Clockwise)

Preceding gene: 161523318

Following gene: 161523328

Centisome position: 4.74

GC content: 66.07

Gene sequence:

>2166_bases
ATGCGCGTGCTTCGCAGCCTCCGGCGCGCGCGGCATCCTCCCGGCCCATCGCCGTCAACCGCAACATCATCCGACATCAT
GTCCGCAGGCCTGAACCCCGCTCAGAACGAAGCGGTGCGCTACCTCGACGGTCCCTGCCTCGTGCTCGCCGGCGCGGGCA
GCGGCAAGACCCGCGTGATCACGCAGAAGATCGCGCACCTGATCGAAGCGAAGGGCTTCGAGCCGCGCCACATCGCGGCC
GTCACGTTCACGAACAAGGCGGCCGCCGAAATGCGCGAACGCGTGTCGAAGCTGCTCGAAGGCAAGACGCTCACGACGCC
CGGCAAGGAAGGGCGCAAGGTGCCCGTGAACCAGCTGACCGTCTGCACGTTCCACTCGCTCGGCGTGCAGATCCTGCGCC
AGGAGGCCGAGCACGTCGGCCTGAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGGATGATCCAGGAGCAG
ATCGGTACGACCGACAAGGGCCTGATCCGCAAGATCCAGAACATCATTTCGCTGTGGAAGAACGGCCTCGTCACGCCCGA
GGAGGCGATGACGATCGCCGCGAACGAGGACGAGCACCAGGCCGCGCTCGTGTACCGCAACTACGTCGCGACGCTGCACG
CGTACCAGGCCGTCGATTTCGACGACCTGATCCGGCTGCCGACCGAGCTGTTCGCGAAGAACGAGCAGGTGCGCGACCGC
TGGCAGAACAAGCTGCGCTACCTGCTGATCGACGAGTACCAGGACACGAACGCGTGCCAGTACCAGCTGCTGAAGCTGCT
CGCGGGCCCGCGCGCCGCGTTCACGGCTGTCGGCGACGACGATCAGGCGATCTACGGCTGGCGCGGCGCGACGCTCGAGA
ACCTGGCGCAGCTCGGCAAGGATTTTCCGAAGCTGCACGTGATCAAGCTCGAGCAGAACTACCGGTCGACGGTGCGGATC
CTGACTGCCGCGAACAACGTCATCGCGAACAACCCGAAGCTGTTCGAGAAGAAGCTGTGGTCCGAGCACGGGATGGGCGA
CGCAATCACCGTCACCGCCTGCAACGACGAGGAGCACGAGGCCGAATCGGTCGTGTTCCGGCTGTCCGCGCACAAGTTCG
AGCGGCGCGCGCAGTTCCGCGACTACGCGATCCTGTATCGCGGCAACTTCCAGGCGCGGATCTTCGAACAGGTGCTGCGG
CGCGAGCGGATTCCGTACGTGCTGTCCGGCGGCCAGTCGTTCTTCGACAAGGCCGAGATCAAGGATCTGTGCGCGTATCT
GCGCCTGATCGCGAACGCCGACGACGATCCCGCGTTCATTCGCGCCGTCACGACGCCGCGCCGCGGGATCGGCAACACGA
CGCTCGAGGCGCTCGGCGCATTCGCCGGGCAGGCGAAGGTGTCGCTGTTCGAGGCCGTTTATATGGGCGGGATCGAGGCG
CGGCTGTCGGCGCGGCAGATCGAGCCGCTGCGGATGTTCTGCGACTTCATCCAGCGGCTGACCGAGCGCGCGGACAAGGA
GCCGGCGTCCGTCGTGCTCGACGACATGATGGAAGCGATCCATTACGAGGCGTATCTGTACGATGCGTTCGACGAGCGGC
AGGCGCAGTCGAAGTGGCAGAACGTGCTCGAGTTCCTCGAATGGCTGAAGCGCAAGGGCACGAAGCCCGAGACGGAAGCC
GTCGACGGCGAGGCCGACGGTTTCCACAACGCGGACGGGCTCGCCGATACCGGCAAGAACCTGCTCGGGCTGATCCAGAC
CGTCGCGCTGATGTCGATGCTCGAAGGCAAGGAAGAGGACCCGGATGCCGTGCGGCTGTCGACCGTCCATGCGTCGAAGG
GGCTCGAGTATCCGCACGTGTTCCTCGTCGGCGTCGAGGAGGGCATCATGCCGCACCGCGGCGGCAGCGAGGACGACGGC
CCGATCGACGACGAGCGGATCGAGGAGGAGCGCCGGCTGATGTACGTCGCGATCACGCGCGCGCAGCGCAGCCTGCATCT
GAACTGGTGCAAGAAGCGCAAGCGGGCGCGCGAGACCGTCGTGTGCGAGCCGTCGCGGTTCATTCCCGAGATGGGCCTCG
ACGATGCGCCGCCGCCGACGCCCGAGGAAGCGCCGATGACGCCGAAGGACCGGCTCGCGAGCCTGAAGGCGCTGCTGCAG
AAGTGA

Upstream 100 bases:

>100_bases
GGCCCATCGTGCGATTGCGGGGCGCGCCGTGCCGGCGGTCGGCGGCGGGCTCCGCGAAACGGGCGCCGCCGACGTGTTAA
CATGCGCGTCCTGTCCGCGC

Downstream 100 bases:

>100_bases
TGCCGCAGCGCCGGTGCGGCGATTGCGCGGACAAACAAAAACCCGCGACGCGCAACGCGTCGCGGGTTTTTTTATCGCGC
GGCGTACGTTATTTCGCTGC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MRVLRSLRRARHPPGPSPSTATSSDIMSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAA
VTFTNKAAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQ
IGTTDKGLIRKIQNIISLWKNGLVTPEEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFAKNEQVRDR
WQNKLRYLLIDEYQDTNACQYQLLKLLAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRI
LTAANNVIANNPKLFEKKLWSEHGMGDAITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLR
RERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEA
RLSARQIEPLRMFCDFIQRLTERADKEPASVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETEA
VDGEADGFHNADGLADTGKNLLGLIQTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDG
PIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDDAPPPTPEEAPMTPKDRLASLKALLQ
K

Sequences:

>Translated_721_residues
MRVLRSLRRARHPPGPSPSTATSSDIMSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAA
VTFTNKAAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQ
IGTTDKGLIRKIQNIISLWKNGLVTPEEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFAKNEQVRDR
WQNKLRYLLIDEYQDTNACQYQLLKLLAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRI
LTAANNVIANNPKLFEKKLWSEHGMGDAITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLR
RERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEA
RLSARQIEPLRMFCDFIQRLTERADKEPASVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETEA
VDGEADGFHNADGLADTGKNLLGLIQTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDG
PIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDDAPPPTPEEAPMTPKDRLASLKALLQ
K
>Mature_721_residues
MRVLRSLRRARHPPGPSPSTATSSDIMSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAA
VTFTNKAAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQ
IGTTDKGLIRKIQNIISLWKNGLVTPEEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFAKNEQVRDR
WQNKLRYLLIDEYQDTNACQYQLLKLLAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRI
LTAANNVIANNPKLFEKKLWSEHGMGDAITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLR
RERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEA
RLSARQIEPLRMFCDFIQRLTERADKEPASVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETEA
VDGEADGFHNADGLADTGKNLLGLIQTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDG
PIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDDAPPPTPEEAPMTPKDRLASLKALLQ
K

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=701, Percent_Identity=47.5035663338088, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI2367296, Length=665, Percent_Identity=35.187969924812, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6322369, Length=728, Percent_Identity=28.7087912087912, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324477, Length=685, Percent_Identity=23.9416058394161, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80993; Mature: 80993

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLRSLRRARHPPGPSPSTATSSDIMSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVI
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCHHHH
TQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLT
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
VCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQNIISLWK
EHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NGLVTPEEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFAKNEQVRDR
CCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHH
WQNKLRYLLIDEYQDTNACQYQLLKLLAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGK
HHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCC
DFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDAITVTACNDEEHE
CCCCEEEEEECCCCHHHEEHHHHCCCHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHH
AESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEI
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
KDLCAYLRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEA
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
RLSARQIEPLRMFCDFIQRLTERADKEPASVVLDDMMEAIHYEAYLYDAFDERQAQSKWQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVLEFLEWLKRKGTKPETEAVDGEADGFHNADGLADTGKNLLGLIQTVALMSMLEGKEED
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDDERIEEERRLMYVAITR
CCCEEEEEEEHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEH
AQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDDAPPPTPEEAPMTPKDRLASLKALLQ
HHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MRVLRSLRRARHPPGPSPSTATSSDIMSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVI
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCHHHH
TQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLT
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
VCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQNIISLWK
EHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NGLVTPEEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFAKNEQVRDR
CCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHH
WQNKLRYLLIDEYQDTNACQYQLLKLLAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGK
HHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCC
DFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDAITVTACNDEEHE
CCCCEEEEEECCCCHHHEEHHHHCCCHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHH
AESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEI
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
KDLCAYLRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEA
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
RLSARQIEPLRMFCDFIQRLTERADKEPASVVLDDMMEAIHYEAYLYDAFDERQAQSKWQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVLEFLEWLKRKGTKPETEAVDGEADGFHNADGLADTGKNLLGLIQTVALMSMLEGKEED
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDDERIEEERRLMYVAITR
CCCEEEEEEEHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEH
AQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDDAPPPTPEEAPMTPKDRLASLKALLQ
HHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]