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The map label for this gene is ilvB [H]

Identifier: 161523316

GI number: 161523316

Start: 151093

End: 152796

Strand: Reverse

Name: ilvB [H]

Synonym: Bmul_0136

Alternate gene names: 161523316

Gene position: 152796-151093 (Counterclockwise)

Preceding gene: 161523319

Following gene: 161523315

Centisome position: 4.43

GC content: 70.66

Gene sequence:

>1704_bases
ATGTCGCATTCCAAGGATCTCGAGCAGCCCGCCACCACCGGCGCCCGACTGCTCGTCGATGCGTTGCTCGCCAATCACGT
CGAACGCGTGTTCTGCGTGCCGGGCGAGAGCTTTCTCGCCGTACTCGATGCGCTCGCGGACGACACCGCGCGCATCCAGA
CGGTCGTCTGCCGCCACGAGGCGGCCGCCGCCAACATGGCCGAAGCGGTCGGCAAGCTGACCGGCCGCCCCGGCATCGCG
TTCGTCACACGCGGGCCCGGCGCGACGCACGCGTCGATCGGCGTGCATACCGCATTCCAGGATTCGACGCCGATGATCCT
GTTCGTCGGCCAGTGCGCACGCGAGCACCTCGATCGCGAGGCGTTCCAGGAAATCGACTACCGCCGGATGTTCGGCCAGA
TGGCGAAATGGGTCGCGCAGATCGACGATCCGCGCCGCATCCCCGAATACCTGAGCCACGCCTTTCACGTCGCGACGTCC
GGGCGGCCGGGGCCGGTCGTGCTCGCGCTGCCGGAAGACGTGCTGTCCGACGTGTGCGCGCCGCAGCCGACGGTGCCCGC
CGCGAAGCGCGTCGCGGCCGCGCCGTCGCCGGCCCAGATCGACGAGCTGCGCGAGCGGCTCGCGCGCGCGGAACGCCCGT
TCGCGATCGTCGGCGGCAGCGGCTGGACGCCCGAGGCCTGCGCGAACCTGCGCACGTTCGCCGAACGCTGGCAGTTGCCG
GTGGGCTGCGCATTCCGGTATCAGGACACGTTCGACAACGCACATCCGAACTACGCGGGCGACGTCGGGCTCGGCATCAA
TCCGGCGCTCGCGAAGCGCATCCGCGACGCCGACTTGCTGCTCGTGCTCGGCCCGCGCCTCGGCGAAGCGACGACCGGCG
GCTATACGCTGCTCGACATCCCGAAGACGCGGCAGACGCTGATCCACGTGCATCAGGGCGCGGACGAGCTCGGCCGCGTC
TATGCGGCCGACCTGCCGATCGTCTCGGGGATGCCGGAGATCGCCGCGCCGCTCGCCGCGCTCGAGCCGCCCGCGCTGCT
TCCGTGGGCCGGCACGGCCGCCGACGCGCACCGCGCCTACCGCGAGTGGCATGCGCCGCTACCGATGCCGGGCGACGTGC
AGCTCGGCGACGTAATGGTGCAGCTGCGCGAGCGGCTGTCGCACGATGCGATCGTGACGAACGGCGCGGGCAATTATGCG
ATCTGGCTGCATCGTCATTTCGCGTACCGGCACTTCCGCTCGCAGCTCGCGCCGACGAGCGGCGCGATGGGCTACGGCGT
GCCGGCCGCGCTCGCCGCGAAGTCGCTCTATCCGTCGCGCGCGGTGGTCGCGCTGGCCGGCGACGGCTGCTTCATGATGG
CCGGCAACGAACTGGCGACCGCGATGCAGTACGGGCTGAACATCGTCGTGATCGTCGTCAACAACGGGCATTTCGGCACG
ATCCGCATGCATCAGGAGCGCAACTATCCGGGCCGCGTGCACGGCACGGGCCTCACGAACCCCGACTTCGCCGCGTATGC
GCGCGCGTTCGGTGCGCATGGCGAGACGGTCGTGCGCACGGCAGAATTCGCGCCGGCGCTCGATCGCGCGCTGACCTGCG
GGCTGCCCGCGCTGATCGAGATCCGCATTCCGCAGGACGCGAGCACGCCGGCCGCGACGCTCGAACAGATCCGCGAGCAA
GGCCGTCGCGCACGCGGCGCATGA

Upstream 100 bases:

>100_bases
TGCGCCCTCCCGTGTTCCGACACTTTTCATCGGCCGGCATACCGAAGCCCCGCCAACGAGCGTAATCTGTACGTCCCGCT
ACCCAGGGAGACGGCAGCCC

Downstream 100 bases:

>100_bases
CGGTGGCCACGCTCGCCGCGCCGGCGGCCGCCGCCCCCGCCGCACTGTCGCACGACGCGGCGCTCGCGTTTCCCGGCACG
CTGCTCGCGCCCGACCGCAC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSHSKDLEQPATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIA
FVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATS
GRPGPVVLALPEDVLSDVCAPQPTVPAAKRVAAAPSPAQIDELRERLARAERPFAIVGGSGWTPEACANLRTFAERWQLP
VGCAFRYQDTFDNAHPNYAGDVGLGINPALAKRIRDADLLLVLGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRV
YAADLPIVSGMPEIAAPLAALEPPALLPWAGTAADAHRAYREWHAPLPMPGDVQLGDVMVQLRERLSHDAIVTNGAGNYA
IWLHRHFAYRHFRSQLAPTSGAMGYGVPAALAAKSLYPSRAVVALAGDGCFMMAGNELATAMQYGLNIVVIVVNNGHFGT
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVVRTAEFAPALDRALTCGLPALIEIRIPQDASTPAATLEQIREQ
GRRARGA

Sequences:

>Translated_567_residues
MSHSKDLEQPATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIA
FVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATS
GRPGPVVLALPEDVLSDVCAPQPTVPAAKRVAAAPSPAQIDELRERLARAERPFAIVGGSGWTPEACANLRTFAERWQLP
VGCAFRYQDTFDNAHPNYAGDVGLGINPALAKRIRDADLLLVLGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRV
YAADLPIVSGMPEIAAPLAALEPPALLPWAGTAADAHRAYREWHAPLPMPGDVQLGDVMVQLRERLSHDAIVTNGAGNYA
IWLHRHFAYRHFRSQLAPTSGAMGYGVPAALAAKSLYPSRAVVALAGDGCFMMAGNELATAMQYGLNIVVIVVNNGHFGT
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVVRTAEFAPALDRALTCGLPALIEIRIPQDASTPAATLEQIREQ
GRRARGA
>Mature_566_residues
SHSKDLEQPATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIAF
VTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSG
RPGPVVLALPEDVLSDVCAPQPTVPAAKRVAAAPSPAQIDELRERLARAERPFAIVGGSGWTPEACANLRTFAERWQLPV
GCAFRYQDTFDNAHPNYAGDVGLGINPALAKRIRDADLLLVLGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRVY
AADLPIVSGMPEIAAPLAALEPPALLPWAGTAADAHRAYREWHAPLPMPGDVQLGDVMVQLRERLSHDAIVTNGAGNYAI
WLHRHFAYRHFRSQLAPTSGAMGYGVPAALAAKSLYPSRAVVALAGDGCFMMAGNELATAMQYGLNIVVIVVNNGHFGTI
RMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVVRTAEFAPALDRALTCGLPALIEIRIPQDASTPAATLEQIREQG
RRARGA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=24.0137221269297, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=24.6846846846847, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=29.4559099437148, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=28.9855072463768, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1786717, Length=540, Percent_Identity=28.1481481481481, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=26.25, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788716, Length=581, Percent_Identity=25.1290877796902, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=26.8292682926829, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=26.8292682926829, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=561, Percent_Identity=23.5294117647059, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=25.7142857142857, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320816, Length=546, Percent_Identity=21.978021978022, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922626, Length=484, Percent_Identity=26.6528925619835, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60994; Mature: 60863

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSKDLEQPATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
AAAANMAEAVGKLTGRPGIAFVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDRE
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHH
AFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSGRPGPVVLALPEDVLSDVCA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHHHHHHCC
PQPTVPAAKRVAAAPSPAQIDELRERLARAERPFAIVGGSGWTPEACANLRTFAERWQLP
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
VGCAFRYQDTFDNAHPNYAGDVGLGINPALAKRIRDADLLLVLGPRLGEATTGGYTLLDI
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEC
PKTRQTLIHVHQGADELGRVYAADLPIVSGMPEIAAPLAALEPPALLPWAGTAADAHRAY
CCHHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
REWHAPLPMPGDVQLGDVMVQLRERLSHDAIVTNGAGNYAIWLHRHFAYRHFRSQLAPTS
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCC
GAMGYGVPAALAAKSLYPSRAVVALAGDGCFMMAGNELATAMQYGLNIVVIVVNNGHFGT
CCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEEECCCEEE
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVVRTAEFAPALDRALTCGLPALIE
EEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCEEEE
IRIPQDASTPAATLEQIREQGRRARGA
EECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHSKDLEQPATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHE
CCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
AAAANMAEAVGKLTGRPGIAFVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDRE
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHH
AFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSGRPGPVVLALPEDVLSDVCA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHHHHHHCC
PQPTVPAAKRVAAAPSPAQIDELRERLARAERPFAIVGGSGWTPEACANLRTFAERWQLP
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
VGCAFRYQDTFDNAHPNYAGDVGLGINPALAKRIRDADLLLVLGPRLGEATTGGYTLLDI
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEC
PKTRQTLIHVHQGADELGRVYAADLPIVSGMPEIAAPLAALEPPALLPWAGTAADAHRAY
CCHHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
REWHAPLPMPGDVQLGDVMVQLRERLSHDAIVTNGAGNYAIWLHRHFAYRHFRSQLAPTS
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCC
GAMGYGVPAALAAKSLYPSRAVVALAGDGCFMMAGNELATAMQYGLNIVVIVVNNGHFGT
CCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEEECCCEEE
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVVRTAEFAPALDRALTCGLPALIE
EEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCEEEE
IRIPQDASTPAATLEQIREQGRRARGA
EECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]