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The map label for this gene is priA [H]

Identifier: 161523293

GI number: 161523293

Start: 118711

End: 120984

Strand: Direct

Name: priA [H]

Synonym: Bmul_0113

Alternate gene names: 161523293

Gene position: 118711-120984 (Clockwise)

Preceding gene: 161523292

Following gene: 161523294

Centisome position: 3.44

GC content: 72.25

Gene sequence:

>2274_bases
ATGAGCGGCACGTATCTGCGCGTCGCGCTCGACCACCCGCTCGCGACGCTGTTCGACTACCGATGCGACGTCGACCCGCC
GCCGGGCCCCGGCACGCTCGTGCAGGTGCCGTTCGGCAAGCGCCGCGCGGTGGGCCTCGTCTGCGAAGTGACGACTCACA
CCGATGTGCCGCCGTCGCGGCTGCGCGCGATCGACGCGGTCTGCACCGAACTGCCGCCGCTGTCGACCGACTGGCTCGCG
CTCGTGTCGTTCGCGGCCGACTACTATCAGCGCGGCCGCGGCGAAGTCGCGCTGCCGGCGCTGCCGCAGGCGCTGCGCGA
CGCGGAGCGCTGGGGGCGGCTGCTCGCGCCCGAAGTGCGATACCGGCTCACCGACGCCGGCCGCGCGGCCCTGCCCGACG
CATTGCCGGCGCGCGCCGCGGCGTTGCGGCGGCTCGCGCAGGCGCTCGCCGACACGGACGCGCTCACGCTGCCCGACGCG
CGCGCGCTGCATCCGAAGGCGGCCGCGACGCTCGACGACTGGGCGGCGCGCGGCTGGGTCGACGTGCAGGAGATCGGCTG
GTCGGACGCGGCTGTGGGCAATCCTGTGCCCAACGGTGTGGATAACCTGTCGACAAGCGGTGGAAGAACTGTGCCGCCGG
CGCTGACCGACCAGCAGGCGGAAGCGCTCGACGCGATCCGGGCGGCGCGGGGCTTCGCGCCGTTCCTGCTGCACGGCGTG
ACCGGCAGCGGCAAGACGGAGGTCTATCTGCACGCGCTCGCGTCGCTGCTCGACACGCGTCCCGATGCGCAGGCGCTCGT
GCTCGTCCCCGAAATCAACCTCACCCCGCAGTTCGAGGCCGCGTTCCGCGCGCGCTTCGCAGGCACGCTCGCCGACGACG
CGATCGTCACGCTCCACAGCGGCCTCGCGGAAGGCGAACGCGCGCGCAACTGGCTCGCCGCGCACACGGGCCGCGCGCGG
ATCGTGCTCGGCACGCGGCTCGCGGTGCTCGCGTCGCTGCCGACGCTCGCGCTGATCGTCGTCGACGAGGAACACGAACC
GGCGTACAAGCAGCAGGAAGGGTTGCGCTATTCGGCGCGCGATCTCGCGGTCTGGCGCGCGAAGCATCTCGACATTCCGG
TCGTGCTCGGCTCCGCGACGCCTTCGCTCGAGAGCTGGTGGCAGGCCGAGCAGGGACGCTACACGCGGCTCACGCTGTCG
CGTCGCGCTGTGGCCGACGCGGTGCTGCCGACGGTGCGGCTGATCGACCTCGAAGAGGAGCGGCGGCGCGGGCGCGCATC
GACGGGCGGGCTGTCCGGGCCGCTGATCGCCGCGCTGAAGACGCGACTCGAGCGCGGCGAGCAGAGCCTCGTGTTCCTGA
ACCGGCGCGGCTACGCGCCGCAGCTCGCCTGCGACGCGTGCGGCTGGGTCGCGGGATGCCCGCGCTGCAGCGCCTATGTC
GTGCTGCACAAACCCGAGCATGCGCTGCGCTGCCATCACTGCGGCTGGGAAGCGCGCATTCCGCGCTCGTGCCCCGAATG
CGGCAACGTCGACATCGCGCCGCTCGGGCGCGGCACGCAGCGTATCGAGGAGACGCTCGCCGAAGCCGTGCCGGGCGCGC
GCGTGCTGCGCATCGACGCGGATAGCACGCGCCGCAAGGGCAGCGCCCAGGCGCTGTTTTCCGACGTGCACGCGGGCGAA
GTCGATATCCTGGTCGGCACGCAGATGATCGCGAAGGGGCACGACTTCCAGCGCGTGTCGCTCGTCGGCGTGCTCAACGC
CGATACCGCGCTGTTCTCGCACGACTTCCGTGCGAGCGAGCGGCTGTTCGCGCAGCTGATGCAGGTCAGCGGCCGCGCCG
GGCGCGCGGGGCTGCCGGGCGAGGTGCTCGTGCAGACGCGCTATCCGCGTCATGCGCTCTATCACGCGCTCGCGCGGCAG
GATTACGTCGGCTTCGCGAATTCGACGCTCGGCGAGCGTCGCGACGCGCATCTGCCGCCGTTCGTCTATCAGGCGCTGCT
GCGCGCGGAAGCGCGCACGCTCGATGCCGCGCTCGCGTTTCTGCAGCAGGCCGCGGCCGCGCTGCCCGGGCTGCCGGGCG
CCGATCGTGTAACGGTCTACGATGCGGTGCCGATGACGATCGTGAAGGTCGCGAACGTCCATCGTGCGCAACTGCTGCTC
GAAAGCGCGTCGCGTGCGGCGCTCCAGCATGCGCTGCGCGCATGGCAGCCGGAACTGCGCGCGCTGAAGGGCGTGCTGCG
GTGGAGCGTCGAGGTCGATCCGCTCGACATCTGA

Upstream 100 bases:

>100_bases
TAGCGCCGAATCTGAAGTTTTACTTTAAGTTCGCAGACGCGATGCGGACGCGCGCCTATACCGTCTGTGCCACCGCCGCA
GCGGCCGCCGGAGCCCCGCG

Downstream 100 bases:

>100_bases
CGCGCCGCAGCGGCGCGCGCTTGCTGCCGGCGCTTGGCGCCGCTTCTTCCTTACACCCGCCGCGGCCACGCGGCCATCCC
AGTGCAACCCGTTTTCGTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MSGTYLRVALDHPLATLFDYRCDVDPPPGPGTLVQVPFGKRRAVGLVCEVTTHTDVPPSRLRAIDAVCTELPPLSTDWLA
LVSFAADYYQRGRGEVALPALPQALRDAERWGRLLAPEVRYRLTDAGRAALPDALPARAAALRRLAQALADTDALTLPDA
RALHPKAAATLDDWAARGWVDVQEIGWSDAAVGNPVPNGVDNLSTSGGRTVPPALTDQQAEALDAIRAARGFAPFLLHGV
TGSGKTEVYLHALASLLDTRPDAQALVLVPEINLTPQFEAAFRARFAGTLADDAIVTLHSGLAEGERARNWLAAHTGRAR
IVLGTRLAVLASLPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKHLDIPVVLGSATPSLESWWQAEQGRYTRLTLS
RRAVADAVLPTVRLIDLEEERRRGRASTGGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYV
VLHKPEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEETLAEAVPGARVLRIDADSTRRKGSAQALFSDVHAGE
VDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARQ
DYVGFANSTLGERRDAHLPPFVYQALLRAEARTLDAALAFLQQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLL
ESASRAALQHALRAWQPELRALKGVLRWSVEVDPLDI

Sequences:

>Translated_757_residues
MSGTYLRVALDHPLATLFDYRCDVDPPPGPGTLVQVPFGKRRAVGLVCEVTTHTDVPPSRLRAIDAVCTELPPLSTDWLA
LVSFAADYYQRGRGEVALPALPQALRDAERWGRLLAPEVRYRLTDAGRAALPDALPARAAALRRLAQALADTDALTLPDA
RALHPKAAATLDDWAARGWVDVQEIGWSDAAVGNPVPNGVDNLSTSGGRTVPPALTDQQAEALDAIRAARGFAPFLLHGV
TGSGKTEVYLHALASLLDTRPDAQALVLVPEINLTPQFEAAFRARFAGTLADDAIVTLHSGLAEGERARNWLAAHTGRAR
IVLGTRLAVLASLPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKHLDIPVVLGSATPSLESWWQAEQGRYTRLTLS
RRAVADAVLPTVRLIDLEEERRRGRASTGGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYV
VLHKPEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEETLAEAVPGARVLRIDADSTRRKGSAQALFSDVHAGE
VDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARQ
DYVGFANSTLGERRDAHLPPFVYQALLRAEARTLDAALAFLQQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLL
ESASRAALQHALRAWQPELRALKGVLRWSVEVDPLDI
>Mature_756_residues
SGTYLRVALDHPLATLFDYRCDVDPPPGPGTLVQVPFGKRRAVGLVCEVTTHTDVPPSRLRAIDAVCTELPPLSTDWLAL
VSFAADYYQRGRGEVALPALPQALRDAERWGRLLAPEVRYRLTDAGRAALPDALPARAAALRRLAQALADTDALTLPDAR
ALHPKAAATLDDWAARGWVDVQEIGWSDAAVGNPVPNGVDNLSTSGGRTVPPALTDQQAEALDAIRAARGFAPFLLHGVT
GSGKTEVYLHALASLLDTRPDAQALVLVPEINLTPQFEAAFRARFAGTLADDAIVTLHSGLAEGERARNWLAAHTGRARI
VLGTRLAVLASLPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKHLDIPVVLGSATPSLESWWQAEQGRYTRLTLSR
RAVADAVLPTVRLIDLEEERRRGRASTGGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVV
LHKPEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEETLAEAVPGARVLRIDADSTRRKGSAQALFSDVHAGEV
DILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARQD
YVGFANSTLGERRDAHLPPFVYQALLRAEARTLDAALAFLQQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLE
SASRAALQHALRAWQPELRALKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=763, Percent_Identity=41.1533420707733, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82039; Mature: 81907

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTYLRVALDHPLATLFDYRCDVDPPPGPGTLVQVPFGKRRAVGLVCEVTTHTDVPPSR
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHH
LRAIDAVCTELPPLSTDWLALVSFAADYYQRGRGEVALPALPQALRDAERWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCHHH
YRLTDAGRAALPDALPARAAALRRLAQALADTDALTLPDARALHPKAAATLDDWAARGWV
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHCCHHHHHHCCCC
DVQEIGWSDAAVGNPVPNGVDNLSTSGGRTVPPALTDQQAEALDAIRAARGFAPFLLHGV
CHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECC
TGSGKTEVYLHALASLLDTRPDAQALVLVPEINLTPQFEAAFRARFAGTLADDAIVTLHS
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
GLAEGERARNWLAAHTGRARIVLGTRLAVLASLPTLALIVVDEEHEPAYKQQEGLRYSAR
CCCCCHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHH
DLAVWRAKHLDIPVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADAVLPTVRLIDLEEE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHCCEEEEECHHH
RRRGRASTGGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYV
HHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEE
VLHKPEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEETLAEAVPGARVLRIDA
EEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECC
DSTRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASE
CCCCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHH
RLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARQDYVGFANSTLGERRDAHLPP
HHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
FVYQALLRAEARTLDAALAFLQQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
ESASRAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
SGTYLRVALDHPLATLFDYRCDVDPPPGPGTLVQVPFGKRRAVGLVCEVTTHTDVPPSR
CCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHH
LRAIDAVCTELPPLSTDWLALVSFAADYYQRGRGEVALPALPQALRDAERWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCHHH
YRLTDAGRAALPDALPARAAALRRLAQALADTDALTLPDARALHPKAAATLDDWAARGWV
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHCCHHHHHHCCCC
DVQEIGWSDAAVGNPVPNGVDNLSTSGGRTVPPALTDQQAEALDAIRAARGFAPFLLHGV
CHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECC
TGSGKTEVYLHALASLLDTRPDAQALVLVPEINLTPQFEAAFRARFAGTLADDAIVTLHS
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
GLAEGERARNWLAAHTGRARIVLGTRLAVLASLPTLALIVVDEEHEPAYKQQEGLRYSAR
CCCCCHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHH
DLAVWRAKHLDIPVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADAVLPTVRLIDLEEE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHCCEEEEECHHH
RRRGRASTGGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYV
HHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEE
VLHKPEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEETLAEAVPGARVLRIDA
EEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECC
DSTRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASE
CCCCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHH
RLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARQDYVGFANSTLGERRDAHLPP
HHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
FVYQALLRAEARTLDAALAFLQQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
ESASRAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]