BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is braG [H]

Identifier: 161523264

GI number: 161523264

Start: 89348

End: 90103

Strand: Direct

Name: braG [H]

Synonym: Bmul_0084

Alternate gene names: 161523264

Gene position: 89348-90103 (Clockwise)

Preceding gene: 161523263

Following gene: 161523265

Centisome position: 2.59

GC content: 66.4

Gene sequence:

>756_bases
ATGAAGCACAGCGAACGGGAGGAACGCGAGTTGAGCGGTGTCGAAAGCGGCACGCCCGCGCTGGAAATCACGGGCCTCGA
GGCCTGGTACGGGGAATCGCACATTCTGCATGGCGTCGATCTGACCGTGCATCGCGGCGAGGTCGTCACGCTGCTCGGCC
GCAACGGCGCGGGCCGCACGACGACGCTGCGCGCGATCATGGGGCTCACGGGCCGCCGCAGCGGCTCGATCAAGGTGGCA
GGCAACGAGACGATCGGTCTCGCGACGCACCGCATCGCGCATTTCGGTGTCGGCTATTGCCCGGAGGAGCGCGGGATCTT
CTCGAGCCTGTCGTGCGAGGAGAACCTGATGCTGCCACCGATGATCGGCGCGCGCGAGAACGCGATGTCGCTCGACGACA
TCTACGCGATGTTTCCGAACCTCGCCTCGCGGCGGCAGAGCCAGGGCACGCGGCTGTCCGGCGGCGAGCAGCAGATGCTC
GCGGTTGCGCGCATCCTGCGCACGGGCGCGAACCTGCTGCTGCTCGACGAGATCTCGGAAGGCCTTGCACCGGTGATCGT
GCAGGCGCTTGCGCGCATGATCGTCGCGCTGAAGTCGCGCGGCTACACGATCGTGATGGTCGAACAGAATTTCCGCTTTG
CCGCACCGCTCGCCGACCGCTTCTACGTGATGGAGCACGGCCGCATCGTCGAACATTTCCGCGCGTCGGAACTGGAAGGC
AAGATGCCGGTCCTGCACGACCTGCTCGGGGTGTGA

Upstream 100 bases:

>100_bases
AGGTGCTCGCCGAGGGTTCGTATGCGGAGGTGTCGAAGAATCCGCTCGTCATCGAGGCTTACATGGGCAGCGCCGACGCG
GCGCTCGCGGGGGCGCACGC

Downstream 100 bases:

>100_bases
CGCCGCCCCGTTTCCTTGCCGCCGGCCGTCGCGGCGCACGAACAACCACAACGCATCAGAACAACAGGAGACGTCAATGA
AAATGAAGACCCTCGCCCAC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKHSEREERELSGVESGTPALEITGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA
GNETIGLATHRIAHFGVGYCPEERGIFSSLSCEENLMLPPMIGARENAMSLDDIYAMFPNLASRRQSQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKSRGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFRASELEG
KMPVLHDLLGV

Sequences:

>Translated_251_residues
MKHSEREERELSGVESGTPALEITGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA
GNETIGLATHRIAHFGVGYCPEERGIFSSLSCEENLMLPPMIGARENAMSLDDIYAMFPNLASRRQSQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKSRGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFRASELEG
KMPVLHDLLGV
>Mature_251_residues
MKHSEREERELSGVESGTPALEITGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA
GNETIGLATHRIAHFGVGYCPEERGIFSSLSCEENLMLPPMIGARENAMSLDDIYAMFPNLASRRQSQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKSRGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFRASELEG
KMPVLHDLLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=235, Percent_Identity=26.8085106382979, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI21536376, Length=200, Percent_Identity=28, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI45446740, Length=201, Percent_Identity=29.8507462686567, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI47078218, Length=201, Percent_Identity=29.8507462686567, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI27881501, Length=219, Percent_Identity=28.7671232876712, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI30795238, Length=219, Percent_Identity=28.7671232876712, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI105990541, Length=193, Percent_Identity=27.4611398963731, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI150417984, Length=223, Percent_Identity=26.9058295964126, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=28.4974093264249, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4505771, Length=225, Percent_Identity=28, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI9961250, Length=225, Percent_Identity=28, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI9961252, Length=225, Percent_Identity=28, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI153792144, Length=198, Percent_Identity=25.2525252525253, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27477115, Length=220, Percent_Identity=27.7272727272727, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI27262624, Length=220, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI27262626, Length=220, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI87082268, Length=211, Percent_Identity=41.2322274881517, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1789593, Length=222, Percent_Identity=31.5315315315315, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788225, Length=230, Percent_Identity=33.4782608695652, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789672, Length=231, Percent_Identity=29.004329004329, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787792, Length=222, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787029, Length=215, Percent_Identity=29.7674418604651, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787089, Length=228, Percent_Identity=30.2631578947368, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=28.7037037037037, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790190, Length=216, Percent_Identity=26.3888888888889, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=25.2, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788897, Length=212, Percent_Identity=28.7735849056604, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=30.379746835443, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787370, Length=222, Percent_Identity=31.0810810810811, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787105, Length=248, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788761, Length=221, Percent_Identity=29.8642533936652, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790525, Length=224, Percent_Identity=26.7857142857143, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=26.3888888888889, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=29.8076923076923, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1786872, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87081791, Length=224, Percent_Identity=28.125, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=229, Percent_Identity=28.3842794759825, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789991, Length=230, Percent_Identity=26.9565217391304, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787712, Length=243, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788644, Length=241, Percent_Identity=29.4605809128631, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786398, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081782, Length=172, Percent_Identity=31.9767441860465, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790467, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788472, Length=231, Percent_Identity=26.8398268398268, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789751, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786698, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790544, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081709, Length=216, Percent_Identity=26.8518518518519, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788210, Length=225, Percent_Identity=24.8888888888889, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788165, Length=212, Percent_Identity=25.4716981132075, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789873, Length=212, Percent_Identity=22.6415094339623, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081834, Length=207, Percent_Identity=25.1207729468599, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994943, Length=241, Percent_Identity=23.2365145228216, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787112, Length=218, Percent_Identity=29.8165137614679, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=241, Percent_Identity=27.8008298755187, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=27.5555555555556, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=27.5555555555556, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=230, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=27.5555555555556, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71983523, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=206, Percent_Identity=26.6990291262136, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=208, Percent_Identity=28.3653846153846, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323959, Length=218, Percent_Identity=24.7706422018349, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648314, Length=213, Percent_Identity=27.2300469483568, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=27.8301886792453, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI116007328, Length=233, Percent_Identity=26.6094420600858, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28574150, Length=233, Percent_Identity=26.6094420600858, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221512771, Length=214, Percent_Identity=27.1028037383178, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24643674, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI85725262, Length=187, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24650853, Length=181, Percent_Identity=27.6243093922652, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24650855, Length=181, Percent_Identity=27.6243093922652, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281362751, Length=181, Percent_Identity=27.6243093922652, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27477; Mature: 27477

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSEREERELSGVESGTPALEITGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT
CCCCHHHHHHHCCCCCCCCEEEEECCHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH
TTLRAIMGLTGRRSGSIKVAGNETIGLATHRIAHFGVGYCPEERGIFSSLSCEENLMLPP
HHHHHHHHCCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
MIGARENAMSLDDIYAMFPNLASRRQSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALARMIVALKSRGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFRASELEG
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHCCC
KMPVLHDLLGV
CCHHHHHHHCC
>Mature Secondary Structure
MKHSEREERELSGVESGTPALEITGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT
CCCCHHHHHHHCCCCCCCCEEEEECCHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH
TTLRAIMGLTGRRSGSIKVAGNETIGLATHRIAHFGVGYCPEERGIFSSLSCEENLMLPP
HHHHHHHHCCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
MIGARENAMSLDDIYAMFPNLASRRQSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALARMIVALKSRGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFRASELEG
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHCCC
KMPVLHDLLGV
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]