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The map label for this gene is outE [H]

Identifier: 161523242

GI number: 161523242

Start: 63940

End: 65436

Strand: Reverse

Name: outE [H]

Synonym: Bmul_0062

Alternate gene names: 161523242

Gene position: 65436-63940 (Counterclockwise)

Preceding gene: 161523243

Following gene: 161523241

Centisome position: 1.9

GC content: 68.74

Gene sequence:

>1497_bases
GTGAGCGACCTGCTGTCCTCCGCCCACGACGGCGCACCCGGCGAACGGCAGCCGCCCTCGCCGCTCGCCGCGCGGCTGCT
GCCGTACGGCTTCGCGAAGAACGGCCAGGTGCTGATCGCGCACCAGCTCGACGACACGCTCGAGGTGTGGATCAGCGATC
GCACGAACGACGCCGCGCTGGCCGAGATCGCACGCAATTTCGGCTCGATCGTCGTGCAGCGTGTGCCGGCCGACGAGCTC
GCGCAGGCGATCAACCACGCGTATGCGCGGCAGGACGGCAGCGCTGCGCAGATCGTCGGCGAGGTCGAAGGCGAAGTCGA
TCTGTCGCGGCTGATGCAGGACATCCCGGAAGTCGAGGATCTGCTCGAATCGGAGGACGACGCGCCGATCATCCGGATGA
TCAACGCGCTGCTGACGCAGGCCGCGCGCGAACAGGCGTCGGACATCCATATCGAACCGTTCGAGAATGCATCGGTCGTG
CGCTTTCGCGTCGACGGCACGCTGCGCGACGTCGTGCGGCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAA
GATCATGGCGCAGCTCGACATCGCGGAAAAGCGTCTGCCGCAGGACGGCCGCATCACGCTGCGCGTCGGCGGCCGACCGG
TCGACGTGCGCGTGTCGACGCTGCCGACCGGCCACGGCGAACGCGCGGTGCTGCGTCTGCTCGAGAAGGACGCGCAGCGG
CTGAACCTCGAAGCGCTCGGGATGGGCCGCGACACGCTCGTGCAGTTCGACAAGCTGATCTCGCGTCCGCACGGCATCGT
GCTCGTCACCGGGCCTACGGGCTCCGGGAAGACGACGACGCTGTACGCGTCGATGTCGCGGCTCGAAACCGCGACGACCA
ATATCATGACCGTCGAGGATCCGATCGAATACGACCTGTCGGGCATCGGCCAGACGCAGGTCAACGAGCGGATCGGGATG
ACCTTCGCGCGCGCGCTGCGCTCGATCCTGCGTCAGGACCCGGACGTGATCATGATCGGCGAAATCCGCGACCTCGAAAC
CGCACAGATCGCGGTGCAGGCCTCGCTCACCGGCCACCTCGTGCTCGCGACGCTGCACACGAACGATGCGGCGTCGGCCG
TCACGCGTCTGACCGACATGGGCGTCGAGCCGTATCTGCTCGCGTCGTCGCTGCTCGGCGTGCTCGCGCAGCGGCTCGTG
CGCCAGCTTTGCCCCGTCTGCAAGGAAGAGCGGCACGAGGACGGCCGCAGCGTGTGGCATCCGGTGGGCTGCGACAAGTG
CGGGCATTCGGGCTACACGGGCCGGCGCGGCGTGTACGAGCTGCTGATCGTCGACGATGCGATCCGCACGCTGATTCACC
GCAACGCGGCCGACGCGGAGATTCTCGCGGCCGGCCGCGCGCAGGGCATGCGCACGCTGCGCGACGACGCGGAGCGCTGG
CTCGCGGCCGGTGCGACGTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCATAG

Upstream 100 bases:

>100_bases
AGATCGCGCCGCCGGCCGCCGAGCAGGCGCCCGCGCAGTCGCAGGGCGTGCCGGCGCAGACGGTGCCGCAGCAGCCGCTG
ACCGCGCCGGGAGCGTCGCA

Downstream 100 bases:

>100_bases
CGCGATGCCCGCATTCCGTTTCGAAGCGATCGATGCGGCCGGCCGTACGCAGAAGGGCGTGGTCGATGCCGACAGCGCGC
GCGCCGCACGCGGGCAGCTG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSDLLSSAHDGAPGERQPPSPLAARLLPYGFAKNGQVLIAHQLDDTLEVWISDRTNDAALAEIARNFGSIVVQRVPADEL
AQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVV
RFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQR
LNLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGM
TFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLV
RQLCPVCKEERHEDGRSVWHPVGCDKCGHSGYTGRRGVYELLIVDDAIRTLIHRNAADAEILAAGRAQGMRTLRDDAERW
LAAGATSLEEVLRVTGGA

Sequences:

>Translated_498_residues
MSDLLSSAHDGAPGERQPPSPLAARLLPYGFAKNGQVLIAHQLDDTLEVWISDRTNDAALAEIARNFGSIVVQRVPADEL
AQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVV
RFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQR
LNLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGM
TFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLV
RQLCPVCKEERHEDGRSVWHPVGCDKCGHSGYTGRRGVYELLIVDDAIRTLIHRNAADAEILAAGRAQGMRTLRDDAERW
LAAGATSLEEVLRVTGGA
>Mature_497_residues
SDLLSSAHDGAPGERQPPSPLAARLLPYGFAKNGQVLIAHQLDDTLEVWISDRTNDAALAEIARNFGSIVVQRVPADELA
QAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVR
FRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMT
FARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVR
QLCPVCKEERHEDGRSVWHPVGCDKCGHSGYTGRRGVYELLIVDDAIRTLIHRNAADAEILAAGRAQGMRTLRDDAERWL
AAGATSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=54.2857142857143, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1786296, Length=439, Percent_Identity=42.369020501139, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=36.8794326241135, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54199; Mature: 54067

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLSSAHDGAPGERQPPSPLAARLLPYGFAKNGQVLIAHQLDDTLEVWISDRTNDAAL
CCHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHH
AEIARNFGSIVVQRVPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVED
HHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCHHHEEHHCCCCHHHHHHHHHCHHHHH
LLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
ALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
LNLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVED
CCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECC
PIEYDLSGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEE
VLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRSVWH
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVGCDKCGHSGYTGRRGVYELLIVDDAIRTLIHRNAADAEILAAGRAQGMRTLRDDAERW
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAAGATSLEEVLRVTGGA
HHHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLLSSAHDGAPGERQPPSPLAARLLPYGFAKNGQVLIAHQLDDTLEVWISDRTNDAAL
CHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHH
AEIARNFGSIVVQRVPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVED
HHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCHHHEEHHCCCCHHHHHHHHHCHHHHH
LLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
ALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
LNLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVED
CCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECC
PIEYDLSGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEE
VLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRSVWH
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVGCDKCGHSGYTGRRGVYELLIVDDAIRTLIHRNAADAEILAAGRAQGMRTLRDDAERW
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAAGATSLEEVLRVTGGA
HHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]