Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 161486630

GI number: 161486630

Start: 1355890

End: 1356942

Strand: Direct

Name: bioB [H]

Synonym: VV1327

Alternate gene names: 161486630

Gene position: 1355890-1356942 (Clockwise)

Preceding gene: 161486631

Following gene: 37679512

Centisome position: 40.42

GC content: 46.44

Gene sequence:

>1053_bases
GTGGAAGTACGTCATAACTGGACTCATGCGCAAGTTCGTGAGTTGATGGAAATGCCTTTTATGGATTTGTTGTTTGAAGC
GCAGCAAGTTCATCGTCAATACCACCCTAAGAACTATGTGCAAGTGAGTACCTTGCTTTCGATTAAAACCGGTGCTTGTC
CAGAAGATTGCAAATACTGCCCGCAAAGCGCTCGTTATCAAACGGATGTTGAAAAAGAGCGATTAATGGAAGTGGAGCGT
GTTCTGGATGCCGCGCAAAAGGCCAAAAATGCCGGTTCGACTCGTTTTTGTATGGGAGCAGCTTGGAAGAATCCGAAAGA
ACGCGACATGCCGCTGCTGACTGACATGATCAAAGGCGTCAAAGATATGGGGCTGGAAACCTGTATGACTCTGGGCATGT
TAACCCCAGAACAAGCTAAGCAACTGGCAAGCGCGGGTTTGGATTATTACAACCACAACCTCGATACCTCACCGGAGTAT
TACGGTAACATTATTACCACACGCACTTATCAAGATCGCCTAGATACCTTATCTCATGTACGTGACGCAGGCATGAAGAT
CTGCTCTGGCGGTATCATCGGTATGGGTGAAAGCGCTAATGATCGCGCTGGGTTGCTTGTTGAGTTGGCCAATCTGCCTA
CTCACCCAGAAAGTGTTCCTATCAACATGCTGGTTAAGGTGAAAGGGACACCTTTAGAAACGGCTGAAGAGGTTGACCCG
TTTGACTTCATCCGTTTAATTGCCGTTGCTCGTATTATGATGCCGACTTCAGCCGTTCGTTTGTCTGCAGGGCGTGAAAA
CATGAATGAACAAATGCAAACCTTGTGTTTTATGGCGGGCGCCAATTCTATCTTTTACGGCTGTAAGCTGCTCACAACGC
CAAACCCTTCCGAAGATAAAGACATGCAGCTGTTTAACAAGCTGGGTATTAACAGTCAGCAAGTCTCACAAAAACCTGAT
GAAATTACAGAAAACGAATTGTTAGATCGCGTAGTCGAAAGCGTGGCTGCTCGCCCAACAAAAGACGATCTGTTTTATGA
CGCAAGCCTTTAA

Upstream 100 bases:

>100_bases
GTAAACTTTGATTTATTTGCTAGTGTTGACAGTTTATCGGGACTCGGTAGACTGTCAACAGAACAAAAATAGCAAGTTAA
CTATTACGCAAGGAATACAC

Downstream 100 bases:

>100_bases
CGAAAGAATCGTGCAAGCCCTGAAGCAGAGACGCGAACAGGGCTTGAATCGCCAAAGTGAAGTGATTTTCTCGGGAAATC
AAACGGTTTTAGAACATCAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MEVRHNWTHAQVRELMEMPFMDLLFEAQQVHRQYHPKNYVQVSTLLSIKTGACPEDCKYCPQSARYQTDVEKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPLLTDMIKGVKDMGLETCMTLGMLTPEQAKQLASAGLDYYNHNLDTSPEY
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESANDRAGLLVELANLPTHPESVPINMLVKVKGTPLETAEEVDP
FDFIRLIAVARIMMPTSAVRLSAGRENMNEQMQTLCFMAGANSIFYGCKLLTTPNPSEDKDMQLFNKLGINSQQVSQKPD
EITENELLDRVVESVAARPTKDDLFYDASL

Sequences:

>Translated_350_residues
MEVRHNWTHAQVRELMEMPFMDLLFEAQQVHRQYHPKNYVQVSTLLSIKTGACPEDCKYCPQSARYQTDVEKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPLLTDMIKGVKDMGLETCMTLGMLTPEQAKQLASAGLDYYNHNLDTSPEY
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESANDRAGLLVELANLPTHPESVPINMLVKVKGTPLETAEEVDP
FDFIRLIAVARIMMPTSAVRLSAGRENMNEQMQTLCFMAGANSIFYGCKLLTTPNPSEDKDMQLFNKLGINSQQVSQKPD
EITENELLDRVVESVAARPTKDDLFYDASL
>Mature_350_residues
MEVRHNWTHAQVRELMEMPFMDLLFEAQQVHRQYHPKNYVQVSTLLSIKTGACPEDCKYCPQSARYQTDVEKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPLLTDMIKGVKDMGLETCMTLGMLTPEQAKQLASAGLDYYNHNLDTSPEY
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESANDRAGLLVELANLPTHPESVPINMLVKVKGTPLETAEEVDP
FDFIRLIAVARIMMPTSAVRLSAGRENMNEQMQTLCFMAGANSIFYGCKLLTTPNPSEDKDMQLFNKLGINSQQVSQKPD
EITENELLDRVVESVAARPTKDDLFYDASL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=350, Percent_Identity=70.2857142857143, Blast_Score=516, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=49.8432601880878, Blast_Score=329, Evalue=5e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39331; Mature: 39331

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRHNWTHAQVRELMEMPFMDLLFEAQQVHRQYHPKNYVQVSTLLSIKTGACPEDCKYC
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCHHHHHC
PQSARYQTDVEKERLMEVERVLDAAQKAKNAGSTRFCMGAAWKNPKERDMPLLTDMIKGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KDMGLETCMTLGMLTPEQAKQLASAGLDYYNHNLDTSPEYYGNIITTRTYQDRLDTLSHV
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHH
RDAGMKICSGGIIGMGESANDRAGLLVELANLPTHPESVPINMLVKVKGTPLETAEEVDP
HHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHCCH
FDFIRLIAVARIMMPTSAVRLSAGRENMNEQMQTLCFMAGANSIFYGCKLLTTPNPSEDK
HHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHEECEEEEECCCCCCCH
DMQLFNKLGINSQQVSQKPDEITENELLDRVVESVAARPTKDDLFYDASL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
>Mature Secondary Structure
MEVRHNWTHAQVRELMEMPFMDLLFEAQQVHRQYHPKNYVQVSTLLSIKTGACPEDCKYC
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCHHHHHC
PQSARYQTDVEKERLMEVERVLDAAQKAKNAGSTRFCMGAAWKNPKERDMPLLTDMIKGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KDMGLETCMTLGMLTPEQAKQLASAGLDYYNHNLDTSPEYYGNIITTRTYQDRLDTLSHV
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHH
RDAGMKICSGGIIGMGESANDRAGLLVELANLPTHPESVPINMLVKVKGTPLETAEEVDP
HHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHCCH
FDFIRLIAVARIMMPTSAVRLSAGRENMNEQMQTLCFMAGANSIFYGCKLLTTPNPSEDK
HHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHEECEEEEECCCCCCCH
DMQLFNKLGINSQQVSQKPDEITENELLDRVVESVAARPTKDDLFYDASL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA