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The map label for this gene is metK [H]

Identifier: 161485728

GI number: 161485728

Start: 704536

End: 705750

Strand: Reverse

Name: metK [H]

Synonym: CT0722

Alternate gene names: 161485728

Gene position: 705750-704536 (Counterclockwise)

Preceding gene: 21673553

Following gene: 21673551

Centisome position: 32.75

GC content: 60.41

Gene sequence:

>1215_bases
ATGTCACATTCAAGGTATTACTTTACCTCGGAATCAGTTTCAGAAGGCCATCCGGACAAGGTGGCCGATCAGATTTCCGA
CGCCGTGCTGGATGAGTTCATCAAACAGGACCCCAACTCCCGCGTCGCCTGCGAAACGTTCGTTACCACCGGTCAGGTGA
TCGTCGGCGGCGAAGTCACCAGCAAAGGCATCGTTGATGTCCAGACCATCGCCCGCAAAACCATTACCGAGATCGGTTAC
ACCAAGGGAGAATACATGTTTGACGCAAACTCCTGCGGCATCCTCTCGGCCCTGCACTCGCAGTCGCCCGACATCAACCG
TGGCGTTGACCGCAAGGAAGAGATCGCCGACGAGTTCGACCGCGTTGGCGCTGGCGACCAGGGCATGATGTTCGGTTACG
CCTGCACCGAGACCCCGGAGCTGATGCCTGCCGCCATCCAGTACGCGCAGGAGCTGGTCAGATTGCTCGCCGAAATCCGC
AAGGAGGGCAAGATCATGACCTACCTCCGCCCCGACGCCAAGAGCCAGGTGACGCTCGAATACGACGGAAACGATAACGT
GCTCCGCGTTGAGGCCGTGGTGGTCTCAACGCAGCACGATCCCGAACCGGCAGGCATGAGCGAAGCCGAGTTCCAGGCCG
TCATCAAGAATGACGTCATCGAAAACGTCATCAAGAAGGTGATCCCCGCCAAGCTGATCGACGAGAACACCAAGTTCCAC
ATCAACCCGACCGGTCGTTTTGAAATCGGCGGTCCTCACGGCGACACCGGTCTGACTGGCCGCAAGATCATTGTTGACAC
CTACGGCGGCGCAGCTCCGCACGGCGGTGGCGCGTTCTCCGGTAAAGACCCGTCGAAGGTTGACCGCAGCGCGGCTTACG
CAGCCCGCCACGTGGCCAAGAACATCGTCGCCGCAGGTCTGGCCGACAAGTGCACCGTGCAGGTCTCCTACGCCATCGGC
GTTGCCCGTCCCATCTCGATCTACATCAACACCCACGGCACCAGCAAGCACGGCCTCTCCGACGAGCAGATCCAGGAGAA
AGCCGAGGCGATCTTCGATCTCCGCCCGCTGGCCATCATCCGCCGCTTCAACCTCGACCGTCCGCACGGCTGGTGCTACC
GCGACACGGCTGCTTACGGCCATTTCGGTCGCGAACAGTTCCCGTGGGAAAAGACCGAGAAGGTTGCCGAGCTGAAAGCC
GCTTTGGGCCTCTGA

Upstream 100 bases:

>100_bases
TCCCTCCGCGACATTCACCTTCACGAGAAAACCGCATCGGCAAACCGCCGGACACCTGCCGTTCTTGTCGTTCGATTAAC
AAAAAACCGAAAGGGAAACT

Downstream 100 bases:

>100_bases
TCAAAAACCCTCTGAAAGCCTCTGCGGACTTGCGTCCCGGAGGCTTTCCCTTATTCTGAAAAAACTGAAAACACGCTGAC
ATCATGACAACAGAAGCAGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSHSRYYFTSESVSEGHPDKVADQISDAVLDEFIKQDPNSRVACETFVTTGQVIVGGEVTSKGIVDVQTIARKTITEIGY
TKGEYMFDANSCGILSALHSQSPDINRGVDRKEEIADEFDRVGAGDQGMMFGYACTETPELMPAAIQYAQELVRLLAEIR
KEGKIMTYLRPDAKSQVTLEYDGNDNVLRVEAVVVSTQHDPEPAGMSEAEFQAVIKNDVIENVIKKVIPAKLIDENTKFH
INPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCTVQVSYAIG
VARPISIYINTHGTSKHGLSDEQIQEKAEAIFDLRPLAIIRRFNLDRPHGWCYRDTAAYGHFGREQFPWEKTEKVAELKA
ALGL

Sequences:

>Translated_404_residues
MSHSRYYFTSESVSEGHPDKVADQISDAVLDEFIKQDPNSRVACETFVTTGQVIVGGEVTSKGIVDVQTIARKTITEIGY
TKGEYMFDANSCGILSALHSQSPDINRGVDRKEEIADEFDRVGAGDQGMMFGYACTETPELMPAAIQYAQELVRLLAEIR
KEGKIMTYLRPDAKSQVTLEYDGNDNVLRVEAVVVSTQHDPEPAGMSEAEFQAVIKNDVIENVIKKVIPAKLIDENTKFH
INPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCTVQVSYAIG
VARPISIYINTHGTSKHGLSDEQIQEKAEAIFDLRPLAIIRRFNLDRPHGWCYRDTAAYGHFGREQFPWEKTEKVAELKA
ALGL
>Mature_403_residues
SHSRYYFTSESVSEGHPDKVADQISDAVLDEFIKQDPNSRVACETFVTTGQVIVGGEVTSKGIVDVQTIARKTITEIGYT
KGEYMFDANSCGILSALHSQSPDINRGVDRKEEIADEFDRVGAGDQGMMFGYACTETPELMPAAIQYAQELVRLLAEIRK
EGKIMTYLRPDAKSQVTLEYDGNDNVLRVEAVVVSTQHDPEPAGMSEAEFQAVIKNDVIENVIKKVIPAKLIDENTKFHI
NPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCTVQVSYAIGV
ARPISIYINTHGTSKHGLSDEQIQEKAEAIFDLRPLAIIRRFNLDRPHGWCYRDTAAYGHFGREQFPWEKTEKVAELKAA
LGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=395, Percent_Identity=52.4050632911392, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI4557737, Length=401, Percent_Identity=51.3715710723192, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=60.0997506234414, Blast_Score=469, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=53.1969309462916, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=53.5532994923858, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=398, Percent_Identity=51.7587939698493, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17538494, Length=394, Percent_Identity=52.7918781725888, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565905, Length=394, Percent_Identity=47.7157360406091, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=51.8950437317784, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=347, Percent_Identity=49.8559077809798, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=50.9493670886076, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=80, Percent_Identity=58.75, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=62, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=399, Percent_Identity=50.3759398496241, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=51.1568123393316, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45552159, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580529, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580537, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580533, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580535, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580531, Length=393, Percent_Identity=51.9083969465649, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=51.6624040920716, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=51.6624040920716, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=50.7936507936508, Blast_Score=325, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=51.6393442622951, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44178; Mature: 44047

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSRYYFTSESVSEGHPDKVADQISDAVLDEFIKQDPNSRVACETFVTTGQVIVGGEVT
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCC
SKGIVDVQTIARKTITEIGYTKGEYMFDANSCGILSALHSQSPDINRGVDRKEEIADEFD
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RVGAGDQGMMFGYACTETPELMPAAIQYAQELVRLLAEIRKEGKIMTYLRPDAKSQVTLE
HCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
YDGNDNVLRVEAVVVSTQHDPEPAGMSEAEFQAVIKNDVIENVIKKVIPAKLIDENTKFH
ECCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEE
INPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARHVAK
ECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NIVAAGLADKCTVQVSYAIGVARPISIYINTHGTSKHGLSDEQIQEKAEAIFDLRPLAII
HHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
RRFNLDRPHGWCYRDTAAYGHFGREQFPWEKTEKVAELKAALGL
HHCCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHSRYYFTSESVSEGHPDKVADQISDAVLDEFIKQDPNSRVACETFVTTGQVIVGGEVT
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCC
SKGIVDVQTIARKTITEIGYTKGEYMFDANSCGILSALHSQSPDINRGVDRKEEIADEFD
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RVGAGDQGMMFGYACTETPELMPAAIQYAQELVRLLAEIRKEGKIMTYLRPDAKSQVTLE
HCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
YDGNDNVLRVEAVVVSTQHDPEPAGMSEAEFQAVIKNDVIENVIKKVIPAKLIDENTKFH
ECCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEE
INPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARHVAK
ECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NIVAAGLADKCTVQVSYAIGVARPISIYINTHGTSKHGLSDEQIQEKAEAIFDLRPLAII
HHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
RRFNLDRPHGWCYRDTAAYGHFGREQFPWEKTEKVAELKAALGL
HHCCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA