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The map label for this gene is ygfK [H]

Identifier: 161407219

GI number: 161407219

Start: 5154055

End: 5155734

Strand: Reverse

Name: ygfK [H]

Synonym: Mmcs_4866

Alternate gene names: 161407219

Gene position: 5155734-5154055 (Counterclockwise)

Preceding gene: 108801830

Following gene: 108801828

Centisome position: 90.37

GC content: 71.73

Gene sequence:

>1680_bases
ATGGGCGTCGAGCGCAGTGGCGACTCGGACACGGACGGGGACGTGCCCGGCGCTGCCTCGGACCTGACCCGGTTACCCGA
CCTGGCGCACGGCACGCCGCGGGCGGGCCCGGTACGCCTGCGCTACCCGGTCTACGTCGATCTGCTCCCGCCGTGCAACG
ACGCCTGCCCCGCAGGCGAGAACATCCAGGCGTGGCTGAGTCACGTCCGCGCCGGGCGTCACGAACAGGCGTGGCGCCGG
CTGGTGTCGGACAATCCGTTCGCCGCCATCCACGGGCGGGTGTGCTACCACCCGTGCGAAACACACTGCAACCGAGGCCA
ATTGGACAGTCCGGTCGCGATCCACGCGGTGGAGCGGTTCCTCGGCGACCTCGCGACGCAGAACCGGTGGGAGTTCGCGC
CTCCGGCCGCCCGCAGCGGTAAGCGGGTGCTGGTCGTCGGTGCCGGCCCGAGCGGGTTGTCGGCGGCCTATCTCCTGGCG
CTGCGCGGACACGACGTCGAGATCCGCGACGCCGGTCCGGAGCCCGGCGGCATGATGCGCTACGGCATCCCCAGCTACCG
GCTCCCGCGCGAGGTGGTCGATGCGGAGGTGGACCGGATCGCGGCGCTGGGGGTGCGGCTCACCTGCGGCCACCAGGTCG
AGGACCTGGCCGCCGAACGCGACAGCGGCGACTTCGACGCGGTCTTCGTCGCAGTGGGCGCCCATCTGGCCAAACGTGTC
GACATCCCCGGCCGCGACGCGGGTGCGCTGCTTGATGCGGTGACCTTCCTGCGCAGTGTGGCCGCCGGGCAGCCCCCGGA
GCTCGGGCGGCATGTGGCCGTGTACGGGGGTGGCGACACCGCGATGGACGTCGCCCGGGTGGCCCGCAGGCTGGGGGCCG
AGGACTCCGTCATCATCTACCGCCGCACCCGCGAGCAGATGAGCGCGCACGCCGAGGAGACCGAGGAGGCCGAACGCGAA
GGTGTGCGCATCAACTGGCTGCGCACCATCACCGCGTTCGATCGCCCCGACCTGCAGGTCGAGGTGATGGACCTCGACGA
ACACGGGCAGCCCCGGCCGACCGGGCGGTTCGAGAAGCTGGCGGCCGACACGGTGATCATGGCGCTCGGGCAGAAGACCG
AGTCGGAGTTCATGCGCACCCTGCCGGGCGTGCGCTTCGACGACGACGGCACCGTGCAGGTGTCGCCGACGCTGATGACC
GGGTGCCCCGGGGTGTTCGCCGGCGGGGACATGGTGCCCGGCGAACGCACCGTGACCGTCGGCGTCGGGCACGGCAAGCG
GGCAGCCCGCCACATCGACGCCTGGCTGGCCGGCCGTTCCGGCGAGCGGCCCCCCAGCCACCCGCCGGTCGCCTTCGACG
ACCTGCACCTGTGGTACTTCGGCGACGCCCCGCAGCGCCCGCAGCCCCACCTCGACGCCGAGCGCAGTGTCGCCGGATTC
GACGAGGTGGTCGGGGGCCTGACCGAGCAGGCCGCGGTATACGAGGCCGCGCGCTGCCTCTCGTGCGGTAACTGCTTCGA
ATGCGACGGCTGTTACGGCGCCTGCCCTGAGGACGCGATCATCAAGGTCGCCGAGGGCCACCACGGGTACGAATTCGTCT
ACGACCGCTGCACCGGCTGTGGCGCCTGCTTCGAGCAGTGTCCGGTACATGCCATCGAGATGCTCCCGGAGACGTCGTGA

Upstream 100 bases:

>100_bases
CGTACGGTTTCCACGGCAATTGGATCACCGGATAGGTCGTCGCCCGCTCGACTATCGCCAACCCGGCCCTTATCGCCGCT
GCGGTGCCTACCCTGATGAA

Downstream 100 bases:

>100_bases
CCCGGACCACCGTGGACGGCAACGAGGCGGTGGCCTCGGTCGCCTACCGCCTCAACGAGGTCTGCTGCATCTACCCGATC
ACCCCGTCGTCCCCGATGGC

Product: putative glutamate synthase (NADPH) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MGVERSGDSDTDGDVPGAASDLTRLPDLAHGTPRAGPVRLRYPVYVDLLPPCNDACPAGENIQAWLSHVRAGRHEQAWRR
LVSDNPFAAIHGRVCYHPCETHCNRGQLDSPVAIHAVERFLGDLATQNRWEFAPPAARSGKRVLVVGAGPSGLSAAYLLA
LRGHDVEIRDAGPEPGGMMRYGIPSYRLPREVVDAEVDRIAALGVRLTCGHQVEDLAAERDSGDFDAVFVAVGAHLAKRV
DIPGRDAGALLDAVTFLRSVAAGQPPELGRHVAVYGGGDTAMDVARVARRLGAEDSVIIYRRTREQMSAHAEETEEAERE
GVRINWLRTITAFDRPDLQVEVMDLDEHGQPRPTGRFEKLAADTVIMALGQKTESEFMRTLPGVRFDDDGTVQVSPTLMT
GCPGVFAGGDMVPGERTVTVGVGHGKRAARHIDAWLAGRSGERPPSHPPVAFDDLHLWYFGDAPQRPQPHLDAERSVAGF
DEVVGGLTEQAAVYEAARCLSCGNCFECDGCYGACPEDAIIKVAEGHHGYEFVYDRCTGCGACFEQCPVHAIEMLPETS

Sequences:

>Translated_559_residues
MGVERSGDSDTDGDVPGAASDLTRLPDLAHGTPRAGPVRLRYPVYVDLLPPCNDACPAGENIQAWLSHVRAGRHEQAWRR
LVSDNPFAAIHGRVCYHPCETHCNRGQLDSPVAIHAVERFLGDLATQNRWEFAPPAARSGKRVLVVGAGPSGLSAAYLLA
LRGHDVEIRDAGPEPGGMMRYGIPSYRLPREVVDAEVDRIAALGVRLTCGHQVEDLAAERDSGDFDAVFVAVGAHLAKRV
DIPGRDAGALLDAVTFLRSVAAGQPPELGRHVAVYGGGDTAMDVARVARRLGAEDSVIIYRRTREQMSAHAEETEEAERE
GVRINWLRTITAFDRPDLQVEVMDLDEHGQPRPTGRFEKLAADTVIMALGQKTESEFMRTLPGVRFDDDGTVQVSPTLMT
GCPGVFAGGDMVPGERTVTVGVGHGKRAARHIDAWLAGRSGERPPSHPPVAFDDLHLWYFGDAPQRPQPHLDAERSVAGF
DEVVGGLTEQAAVYEAARCLSCGNCFECDGCYGACPEDAIIKVAEGHHGYEFVYDRCTGCGACFEQCPVHAIEMLPETS
>Mature_558_residues
GVERSGDSDTDGDVPGAASDLTRLPDLAHGTPRAGPVRLRYPVYVDLLPPCNDACPAGENIQAWLSHVRAGRHEQAWRRL
VSDNPFAAIHGRVCYHPCETHCNRGQLDSPVAIHAVERFLGDLATQNRWEFAPPAARSGKRVLVVGAGPSGLSAAYLLAL
RGHDVEIRDAGPEPGGMMRYGIPSYRLPREVVDAEVDRIAALGVRLTCGHQVEDLAAERDSGDFDAVFVAVGAHLAKRVD
IPGRDAGALLDAVTFLRSVAAGQPPELGRHVAVYGGGDTAMDVARVARRLGAEDSVIIYRRTREQMSAHAEETEEAEREG
VRINWLRTITAFDRPDLQVEVMDLDEHGQPRPTGRFEKLAADTVIMALGQKTESEFMRTLPGVRFDDDGTVQVSPTLMTG
CPGVFAGGDMVPGERTVTVGVGHGKRAARHIDAWLAGRSGERPPSHPPVAFDDLHLWYFGDAPQRPQPHLDAERSVAGFD
EVVGGLTEQAAVYEAARCLSCGNCFECDGCYGACPEDAIIKVAEGHHGYEFVYDRCTGCGACFEQCPVHAIEMLPETS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=26.8722466960352, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1789243, Length=512, Percent_Identity=30.859375, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788468, Length=392, Percent_Identity=31.1224489795918, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789606, Length=477, Percent_Identity=27.8825995807128, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788811, Length=420, Percent_Identity=30.4761904761905, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI87082180, Length=423, Percent_Identity=30.4964539007092, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17570289, Length=452, Percent_Identity=26.3274336283186, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=436, Percent_Identity=26.1467889908257, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=30.625, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=257, Percent_Identity=29.5719844357977, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24665547, Length=430, Percent_Identity=29.5348837209302, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24665543, Length=430, Percent_Identity=29.5348837209302, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI28574881, Length=430, Percent_Identity=29.5348837209302, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24665539, Length=430, Percent_Identity=29.5348837209302, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24640763, Length=436, Percent_Identity=27.2935779816514, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18858217, Length=436, Percent_Identity=27.2935779816514, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 60415; Mature: 60284

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVERSGDSDTDGDVPGAASDLTRLPDLAHGTPRAGPVRLRYPVYVDLLPPCNDACPAGE
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCC
NIQAWLSHVRAGRHEQAWRRLVSDNPFAAIHGRVCYHPCETHCNRGQLDSPVAIHAVERF
CHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEECHHHHCCCCCCCCCCHHHHHHHHH
LGDLATQNRWEFAPPAARSGKRVLVVGAGPSGLSAAYLLALRGHDVEIRDAGPEPGGMMR
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCCCEEE
YGIPSYRLPREVVDAEVDRIAALGVRLTCGHQVEDLAAERDSGDFDAVFVAVGAHLAKRV
ECCCCCCCCHHHHHHHHHHHHHHCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHC
DIPGRDAGALLDAVTFLRSVAAGQPPELGRHVAVYGGGDTAMDVARVARRLGAEDSVIIY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEE
RRTREQMSAHAEETEEAEREGVRINWLRTITAFDRPDLQVEVMDLDEHGQPRPTGRFEKL
ECHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHH
AADTVIMALGQKTESEFMRTLPGVRFDDDGTVQVSPTLMTGCPGVFAGGDMVPGERTVTV
HHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEECCHHCCCCCCCCCCCCCCCCCEEEEE
GVGHGKRAARHIDAWLAGRSGERPPSHPPVAFDDLHLWYFGDAPQRPQPHLDAERSVAGF
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEECCCCCCCCCCCCCHHHHCCH
DEVVGGLTEQAAVYEAARCLSCGNCFECDGCYGACPEDAIIKVAEGHHGYEFVYDRCTGC
HHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHEEEEECCCCCHHHHHHHHCCH
GACFEQCPVHAIEMLPETS
HHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
GVERSGDSDTDGDVPGAASDLTRLPDLAHGTPRAGPVRLRYPVYVDLLPPCNDACPAGE
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCC
NIQAWLSHVRAGRHEQAWRRLVSDNPFAAIHGRVCYHPCETHCNRGQLDSPVAIHAVERF
CHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEECHHHHCCCCCCCCCCHHHHHHHHH
LGDLATQNRWEFAPPAARSGKRVLVVGAGPSGLSAAYLLALRGHDVEIRDAGPEPGGMMR
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCCCEEE
YGIPSYRLPREVVDAEVDRIAALGVRLTCGHQVEDLAAERDSGDFDAVFVAVGAHLAKRV
ECCCCCCCCHHHHHHHHHHHHHHCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHC
DIPGRDAGALLDAVTFLRSVAAGQPPELGRHVAVYGGGDTAMDVARVARRLGAEDSVIIY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEE
RRTREQMSAHAEETEEAEREGVRINWLRTITAFDRPDLQVEVMDLDEHGQPRPTGRFEKL
ECHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHH
AADTVIMALGQKTESEFMRTLPGVRFDDDGTVQVSPTLMTGCPGVFAGGDMVPGERTVTV
HHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEECCHHCCCCCCCCCCCCCCCCCEEEEE
GVGHGKRAARHIDAWLAGRSGERPPSHPPVAFDDLHLWYFGDAPQRPQPHLDAERSVAGF
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEECCCCCCCCCCCCCHHHHCCH
DEVVGGLTEQAAVYEAARCLSCGNCFECDGCYGACPEDAIIKVAEGHHGYEFVYDRCTGC
HHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHEEEEECCCCCHHHHHHHHCCH
GACFEQCPVHAIEMLPETS
HHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]