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The map label for this gene is nfnB [H]

Identifier: 161349965

GI number: 161349965

Start: 1913671

End: 1914324

Strand: Direct

Name: nfnB [H]

Synonym: ACIAD1923

Alternate gene names: 161349965

Gene position: 1913671-1914324 (Clockwise)

Preceding gene: 50085062

Following gene: 50085068

Centisome position: 53.18

GC content: 40.67

Gene sequence:

>654_bases
ATGAACTTACTAGAGACCGCAAAAAGTCGTTATACCACCAAAGCTTATGATCGCAGTAAAATAATTCCCTTAGATCAATT
TGAGCGTTTACTCAAGGTTTTGCGTTTAACTCCCTCTTCTGTCAATATTCAGCCGTGGCATTTCTTAATCGCAGAAAGTG
AGCAAGCTAAAGCGCGTGTTGCCAAAGCCATGACTGGACGTTATGCCTATAATGCACCCAAAGTGCTAGAGTCTTCACAT
ACCATTGTTTTTTGCACCCGCACTGATGTCACACATGAACATCTTGAACAACTTCTTGAGCAAGATGATTTAAGCGGACG
TTTTAAAGATGAAAGTGCAAAAGAAGGCCAACGTCAGACCCGTGCAGGCTATGTCGAATATTATCGCAATGAGCAACAAA
ACCTGTATGGATGGATGGAAAACCAAACCTTTATCGCGCTCGGGCAGCTTTTATTGGCAGCGGGGTTAGAAGATGTTGAT
GCAACACCAATGGGTGGTTTCGATGAAGATATCTTAAATCAAGAATTTGGATTAACAGAAAAAGGGTTTCGTGCGTCTGT
TGTAGTTGCATTAGGTTATCGCAGTGAAGCTGACTTTAATGCAAAATTACCGAAATCACGTTTAAGCGATGACATCATTT
TTACTAAAATTTAA

Upstream 100 bases:

>100_bases
CTATTTTGTTACCTCAATTTTTATTTGAAAGCATTTCATCTGATAATTTTTCGATTTATGATCATGTTTTATCTAACCTT
TAAATATGAGGGACATGATC

Downstream 100 bases:

>100_bases
CCTAGAAAAAGGTCATCTGGTCTATTAATAACCCGATGACCTTGTTCACATCGCTTGAGCAAGACAAATAATTCCGACCA
TTCCCAATACTAAACCCATG

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NR [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNLLETAKSRYTTKAYDRSKIIPLDQFERLLKVLRLTPSSVNIQPWHFLIAESEQAKARVAKAMTGRYAYNAPKVLESSH
TIVFCTRTDVTHEHLEQLLEQDDLSGRFKDESAKEGQRQTRAGYVEYYRNEQQNLYGWMENQTFIALGQLLLAAGLEDVD
ATPMGGFDEDILNQEFGLTEKGFRASVVVALGYRSEADFNAKLPKSRLSDDIIFTKI

Sequences:

>Translated_217_residues
MNLLETAKSRYTTKAYDRSKIIPLDQFERLLKVLRLTPSSVNIQPWHFLIAESEQAKARVAKAMTGRYAYNAPKVLESSH
TIVFCTRTDVTHEHLEQLLEQDDLSGRFKDESAKEGQRQTRAGYVEYYRNEQQNLYGWMENQTFIALGQLLLAAGLEDVD
ATPMGGFDEDILNQEFGLTEKGFRASVVVALGYRSEADFNAKLPKSRLSDDIIFTKI
>Mature_217_residues
MNLLETAKSRYTTKAYDRSKIIPLDQFERLLKVLRLTPSSVNIQPWHFLIAESEQAKARVAKAMTGRYAYNAPKVLESSH
TIVFCTRTDVTHEHLEQLLEQDDLSGRFKDESAKEGQRQTRAGYVEYYRNEQQNLYGWMENQTFIALGQLLLAAGLEDVD
ATPMGGFDEDILNQEFGLTEKGFRASVVVALGYRSEADFNAKLPKSRLSDDIIFTKI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=40.5529953917051, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24734; Mature: 24734

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLETAKSRYTTKAYDRSKIIPLDQFERLLKVLRLTPSSVNIQPWHFLIAESEQAKARV
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHH
AKAMTGRYAYNAPKVLESSHTIVFCTRTDVTHEHLEQLLEQDDLSGRFKDESAKEGQRQT
HHHHHCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RAGYVEYYRNEQQNLYGWMENQTFIALGQLLLAAGLEDVDATPMGGFDEDILNQEFGLTE
HHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCC
KGFRASVVVALGYRSEADFNAKLPKSRLSDDIIFTKI
CCCCEEEEEEECCCCCCCCCCCCCHHHCCCCEEEECC
>Mature Secondary Structure
MNLLETAKSRYTTKAYDRSKIIPLDQFERLLKVLRLTPSSVNIQPWHFLIAESEQAKARV
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHH
AKAMTGRYAYNAPKVLESSHTIVFCTRTDVTHEHLEQLLEQDDLSGRFKDESAKEGQRQT
HHHHHCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RAGYVEYYRNEQQNLYGWMENQTFIALGQLLLAAGLEDVDATPMGGFDEDILNQEFGLTE
HHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCC
KGFRASVVVALGYRSEADFNAKLPKSRLSDDIIFTKI
CCCCEEEEEEECCCCCCCCCCCCCHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1999406; 1999405 [H]