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The map label for this gene is hisH

Identifier: 16127965

GI number: 16127965

Start: 3990583

End: 3991224

Strand: Direct

Name: hisH

Synonym: CC_3735

Alternate gene names: 16127965

Gene position: 3990583-3991224 (Clockwise)

Preceding gene: 16127964

Following gene: 16127966

Centisome position: 99.34

GC content: 66.51

Gene sequence:

>642_bases
ATGCAGACCGTCGCCCTGATCGATTACGGGTCGGGCAACCTGCGTTCGGCCGAGAAGGCCCTGCGTGAGGCGGCCCGACG
CCGCGCGCTGGACGCCGACATCGTTGTCACCGCCGACCCTGACCTTGTCGCCAAGGCCGATCGCGTCTTCCTGCCCGGCG
TCGGGGCCTTCGCCTCGTGCCGCGCCGGGCTTGACGCCACCGGCGTCTATGAGGCGATGAACCAGGCCGTACACGGACGC
GGCGTGCCGTTCATGGGCATCTGCGTCGGCCACCAGCTCCTGGCCACCGAAGGGCTGGAGTTCGGCGTCACCCCCGGCCT
GAACTGGATTCAGGGCCAGGTGAAGAAGCTCGAGCCCAACGATCCGACGCTGACCATCCCGCACATGGGCTGGAACGCGA
TCAGCCTCGTGCGTCCTCATGCCCTGTTCGCGGGCATCGACGACGGCGCCCACATGTATTTCGCCAACTCGTTCGCCCTG
ACCCCTTCCCATGTGGAAGATGTCGTCGCGACCGCCGACCACGGCGGACCGTTCACGGCCGCTGTGGCCAAGGACAATGT
CGCGGGCGTACAGTTCCACCCTGAAAAATCACAAGCATCAGGGCTCGCCCTGCTCGCCAACTTCCTGGAATGGCGCCCAT
GA

Upstream 100 bases:

>100_bases
CAAGGCCCTGGCCCGGGCGCTGCGTCAGGCGGTCGAGATCGACCCCAAGACCGGCGGCCAGGCTCCGTCCACCAAGGGCG
TGCTGTAGGAACCTGATTTC

Downstream 100 bases:

>100_bases
TCCTCTATCCCGCCATCGACCTGAAGGACGGCCAGTGCGTGCGCCTGCTGCATGGCGACATGGACAAGGCCACGGTCTTC
AACACCTCGCCCGCCGACCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQTVALIDYGSGNLRSAEKALREAARRRALDADIVVTADPDLVAKADRVFLPGVGAFASCRAGLDATGVYEAMNQAVHGR
GVPFMGICVGHQLLATEGLEFGVTPGLNWIQGQVKKLEPNDPTLTIPHMGWNAISLVRPHALFAGIDDGAHMYFANSFAL
TPSHVEDVVATADHGGPFTAAVAKDNVAGVQFHPEKSQASGLALLANFLEWRP

Sequences:

>Translated_213_residues
MQTVALIDYGSGNLRSAEKALREAARRRALDADIVVTADPDLVAKADRVFLPGVGAFASCRAGLDATGVYEAMNQAVHGR
GVPFMGICVGHQLLATEGLEFGVTPGLNWIQGQVKKLEPNDPTLTIPHMGWNAISLVRPHALFAGIDDGAHMYFANSFAL
TPSHVEDVVATADHGGPFTAAVAKDNVAGVQFHPEKSQASGLALLANFLEWRP
>Mature_213_residues
MQTVALIDYGSGNLRSAEKALREAARRRALDADIVVTADPDLVAKADRVFLPGVGAFASCRAGLDATGVYEAMNQAVHGR
GVPFMGICVGHQLLATEGLEFGVTPGLNWIQGQVKKLEPNDPTLTIPHMGWNAISLVRPHALFAGIDDGAHMYFANSFAL
TPSHVEDVVATADHGGPFTAAVAKDNVAGVQFHPEKSQASGLALLANFLEWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=39.4230769230769, Blast_Score=130, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=221, Percent_Identity=32.579185520362, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CAUCR (Q9A231)

Other databases:

- EMBL:   AE005673
- PIR:   E87712
- RefSeq:   NP_422529.1
- ProteinModelPortal:   Q9A231
- SMR:   Q9A231
- GeneID:   940607
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_3735
- NMPDR:   fig|190650.1.peg.3709
- TIGR:   CC_3735
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13146
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22581; Mature: 22581

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 193-193 ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVALIDYGSGNLRSAEKALREAARRRALDADIVVTADPDLVAKADRVFLPGVGAFASC
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCEEEECCCCHHHHH
RAGLDATGVYEAMNQAVHGRGVPFMGICVGHQLLATEGLEFGVTPGLNWIQGQVKKLEPN
HCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHCCCCCCCCCCCHHHHCCCEEECCC
DPTLTIPHMGWNAISLVRPHALFAGIDDGAHMYFANSFALTPSHVEDVVATADHGGPFTA
CCEEEECCCCCCEEEHCCCCCEEEECCCCCEEEEECCEEECHHHHHHHHHHCCCCCCEEE
AVAKDNVAGVQFHPEKSQASGLALLANFLEWRP
EEECCCCCCEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTVALIDYGSGNLRSAEKALREAARRRALDADIVVTADPDLVAKADRVFLPGVGAFASC
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCEEEECCCCHHHHH
RAGLDATGVYEAMNQAVHGRGVPFMGICVGHQLLATEGLEFGVTPGLNWIQGQVKKLEPN
HCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHCCCCCCCCCCCHHHHCCCEEECCC
DPTLTIPHMGWNAISLVRPHALFAGIDDGAHMYFANSFALTPSHVEDVVATADHGGPFTA
CCEEEECCCCCCEEEHCCCCCEEEECCCCCEEEEECCEEECHHHHHHHHHHCCCCCCEEE
AVAKDNVAGVQFHPEKSQASGLALLANFLEWRP
EEECCCCCCEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647