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The map label for this gene is ubiB [H]

Identifier: 16127939

GI number: 16127939

Start: 3963915

End: 3965423

Strand: Direct

Name: ubiB [H]

Synonym: CC_3709

Alternate gene names: 16127939

Gene position: 3963915-3965423 (Clockwise)

Preceding gene: 16127938

Following gene: 16127942

Centisome position: 98.68

GC content: 69.72

Gene sequence:

>1509_bases
GTGGCCACGCTCGAAGCCTTCTGGCGCCTGACCGGCGCGGGCTGGGCCTTGGTCCGGGCCGACGCTCTGATCCCGCGTGA
ACTGGAACCCCTGCTTCCCCCCAGCGCGAAGCTGGCTGGGCGCGTCCTGCGCCTCTTCGCAGGCCCGGCCGCCCGCAAGG
GACGGCCGGGGGAACGCCTGGCGGCGGTGCTCGAACGTCAAGGGCCGGCCGCAATCAAGATGGGCCAGTTCCTGTCGACC
CGCGCCGATATCTTCGGCGCGGCCTTCGCCGATGACCTGTCGCGCCTGAAGGACCGGCTGCCGGCCTTTCCACTGAGCGT
CGCCAAGGCCGAGATCGCCCGCAACCTCGGCAAGCCGCTGGATGAGATCTTCGCCGAGATCGGTGAACCGGTGGCCGCCG
CCTCTCTGGCCCAGGCCCACCCCGCAACGCTGGTCGACGGCCAAAAGGTCGCGGTCAAGGTCCTGCGCCCGGGCGTCGAG
CGGCAGGTCGCCCGAGACACCGCCGTCCTGCGCCTGGCCGCGCGTCTCGCCGAGACCCTCGTCCCAGTCTCGCGACGTCT
GCGTCCGACCGAGTTCGTCGAGGTCGTGATCCGCGCACTCGAGCTTGAGATGGACCTGCGCTTCGAGGCCGCCGGCTGCG
CCGAGCTGGGCGAGGCCATGGCCAAAGACCCCTATATGCGCGCGCCCGCCGTCTGCTGGGAGGGCGTCGGCAAACGCGTC
CTGACCCTGAGCTGGGCGGAAGGCGCACCCTTATCCGACCCCGCCGCACTGGATCTGCCAGGCCTGGACCGCAAGGCGCT
GGCCGAGAACGTCACGCGCGGTTTCCTCGCCCAAGCGCTGGATCACGGCCTGTTCCACGCCGACCTCCACGAGGGCAATC
TGTTCATCGCCGCGCCGGCGGCGATCACGGCGGTGGACTATGGCATTGTCGGTCGCCTGGGGCCCGGCGAGCGCCGCTAC
CTCGCCGAGATCCTCTACGGCTTTCTCAATCGTGACTATGCGCGCGTGGCCAAGATCCATTTCGACGCCGGTTATGTGCC
CGCCCACCAGGATATGGACGCCTTCGCCCAAGCCCTGCGCGCTGTCGGCGAGCCGGTGTTTGGCCGCAACGCGCGCGAGG
TCTCGATGGGCCGACTGCTGGCGCAGCTGTTCGAGATTACCGCCCTGTTCGATATGGCGCTGCGCCCAGAGCTCGTTCTG
CTTCAGAAGACCATGGTCACCGTTGAAGGCGTCGCTCGCCGGATCGATCCCACCCACGACCTTTGGGCGGCCGCAGATCC
GGTCGTACGGCGGTGGATCGGCCGTGAACTCTCCCCCGCCGCCAAGGCGCGAGACTTCGCCGAAGAGGCGATACGGGCGA
TCAAGGCTCTCGCGCGGCTGGCGGAGACCCCCACCACACCCCCGCCCGTCGCCGCCGAACGCCTTCACACTTGGCCCCTG
CTCTGGTTTCTGGTCGGCGCGGCCACGGCGGGAGCGGCCTTTGTGACCGGTGTGCTCCTGGCCCGCTAG

Upstream 100 bases:

>100_bases
TTCGCGGGCATGATCGAGGCCGCCGGATTCAAGCGCGTGACCTTCACCAACTTTACCGGCGGCGTCGCGGCCCTGCACCA
GGGCTGGGCGATCTAGGACC

Downstream 100 bases:

>100_bases
GATCAATCATCCAGAGGCAGCCGGCGCACCTCCCGTTCGTTGAGCGGTTGGGCTCGTGGCCTCGACGTTGAAACTCCCGA
CGATCGCCTCATAAAGCGTC

Product: ubiquinone biosynthesis protein AarF

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MATLEAFWRLTGAGWALVRADALIPRELEPLLPPSAKLAGRVLRLFAGPAARKGRPGERLAAVLERQGPAAIKMGQFLST
RADIFGAAFADDLSRLKDRLPAFPLSVAKAEIARNLGKPLDEIFAEIGEPVAAASLAQAHPATLVDGQKVAVKVLRPGVE
RQVARDTAVLRLAARLAETLVPVSRRLRPTEFVEVVIRALELEMDLRFEAAGCAELGEAMAKDPYMRAPAVCWEGVGKRV
LTLSWAEGAPLSDPAALDLPGLDRKALAENVTRGFLAQALDHGLFHADLHEGNLFIAAPAAITAVDYGIVGRLGPGERRY
LAEILYGFLNRDYARVAKIHFDAGYVPAHQDMDAFAQALRAVGEPVFGRNAREVSMGRLLAQLFEITALFDMALRPELVL
LQKTMVTVEGVARRIDPTHDLWAAADPVVRRWIGRELSPAAKARDFAEEAIRAIKALARLAETPTTPPPVAAERLHTWPL
LWFLVGAATAGAAFVTGVLLAR

Sequences:

>Translated_502_residues
MATLEAFWRLTGAGWALVRADALIPRELEPLLPPSAKLAGRVLRLFAGPAARKGRPGERLAAVLERQGPAAIKMGQFLST
RADIFGAAFADDLSRLKDRLPAFPLSVAKAEIARNLGKPLDEIFAEIGEPVAAASLAQAHPATLVDGQKVAVKVLRPGVE
RQVARDTAVLRLAARLAETLVPVSRRLRPTEFVEVVIRALELEMDLRFEAAGCAELGEAMAKDPYMRAPAVCWEGVGKRV
LTLSWAEGAPLSDPAALDLPGLDRKALAENVTRGFLAQALDHGLFHADLHEGNLFIAAPAAITAVDYGIVGRLGPGERRY
LAEILYGFLNRDYARVAKIHFDAGYVPAHQDMDAFAQALRAVGEPVFGRNAREVSMGRLLAQLFEITALFDMALRPELVL
LQKTMVTVEGVARRIDPTHDLWAAADPVVRRWIGRELSPAAKARDFAEEAIRAIKALARLAETPTTPPPVAAERLHTWPL
LWFLVGAATAGAAFVTGVLLAR
>Mature_501_residues
ATLEAFWRLTGAGWALVRADALIPRELEPLLPPSAKLAGRVLRLFAGPAARKGRPGERLAAVLERQGPAAIKMGQFLSTR
ADIFGAAFADDLSRLKDRLPAFPLSVAKAEIARNLGKPLDEIFAEIGEPVAAASLAQAHPATLVDGQKVAVKVLRPGVER
QVARDTAVLRLAARLAETLVPVSRRLRPTEFVEVVIRALELEMDLRFEAAGCAELGEAMAKDPYMRAPAVCWEGVGKRVL
TLSWAEGAPLSDPAALDLPGLDRKALAENVTRGFLAQALDHGLFHADLHEGNLFIAAPAAITAVDYGIVGRLGPGERRYL
AEILYGFLNRDYARVAKIHFDAGYVPAHQDMDAFAQALRAVGEPVFGRNAREVSMGRLLAQLFEITALFDMALRPELVLL
QKTMVTVEGVARRIDPTHDLWAAADPVVRRWIGRELSPAAKARDFAEEAIRAIKALARLAETPTTPPPVAAERLHTWPLL
WFLVGAATAGAAFVTGVLLAR

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254938, Length=294, Percent_Identity=25.1700680272109, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI41393593, Length=271, Percent_Identity=27.6752767527675, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI27363457, Length=324, Percent_Identity=27.1604938271605, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI217035081, Length=233, Percent_Identity=25.7510729613734, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI217416386, Length=269, Percent_Identity=27.8810408921933, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI34147522, Length=246, Percent_Identity=28.0487804878049, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI2367309, Length=427, Percent_Identity=37.4707259953162, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17559152, Length=291, Percent_Identity=24.7422680412371, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32565180, Length=318, Percent_Identity=24.5283018867925, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6325148, Length=435, Percent_Identity=21.8390804597701, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321319, Length=321, Percent_Identity=24.9221183800623, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24662575, Length=319, Percent_Identity=28.2131661442006, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI22024280, Length=280, Percent_Identity=24.6428571428571, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 54295; Mature: 54164

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLEAFWRLTGAGWALVRADALIPRELEPLLPPSAKLAGRVLRLFAGPAARKGRPGERL
CCCHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
AAVLERQGPAAIKMGQFLSTRADIFGAAFADDLSRLKDRLPAFPLSVAKAEIARNLGKPL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
DEIFAEIGEPVAAASLAQAHPATLVDGQKVAVKVLRPGVERQVARDTAVLRLAARLAETL
HHHHHHHCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VPVSRRLRPTEFVEVVIRALELEMDLRFEAAGCAELGEAMAKDPYMRAPAVCWEGVGKRV
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEE
LTLSWAEGAPLSDPAALDLPGLDRKALAENVTRGFLAQALDHGLFHADLHEGNLFIAAPA
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCH
AITAVDYGIVGRLGPGERRYLAEILYGFLNRDYARVAKIHFDAGYVPAHQDMDAFAQALR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCCCCHHHHHHHHH
AVGEPVFGRNAREVSMGRLLAQLFEITALFDMALRPELVLLQKTMVTVEGVARRIDPTHD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
LWAAADPVVRRWIGRELSPAAKARDFAEEAIRAIKALARLAETPTTPPPVAAERLHTWPL
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LWFLVGAATAGAAFVTGVLLAR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATLEAFWRLTGAGWALVRADALIPRELEPLLPPSAKLAGRVLRLFAGPAARKGRPGERL
CCHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
AAVLERQGPAAIKMGQFLSTRADIFGAAFADDLSRLKDRLPAFPLSVAKAEIARNLGKPL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
DEIFAEIGEPVAAASLAQAHPATLVDGQKVAVKVLRPGVERQVARDTAVLRLAARLAETL
HHHHHHHCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VPVSRRLRPTEFVEVVIRALELEMDLRFEAAGCAELGEAMAKDPYMRAPAVCWEGVGKRV
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEE
LTLSWAEGAPLSDPAALDLPGLDRKALAENVTRGFLAQALDHGLFHADLHEGNLFIAAPA
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCH
AITAVDYGIVGRLGPGERRYLAEILYGFLNRDYARVAKIHFDAGYVPAHQDMDAFAQALR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCCCCHHHHHHHHH
AVGEPVFGRNAREVSMGRLLAQLFEITALFDMALRPELVLLQKTMVTVEGVARRIDPTHD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
LWAAADPVVRRWIGRELSPAAKARDFAEEAIRAIKALARLAETPTTPPPVAAERLHTWPL
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LWFLVGAATAGAAFVTGVLLAR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]