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The map label for this gene is rlmH

Identifier: 16127663

GI number: 16127663

Start: 3676620

End: 3677087

Strand: Direct

Name: rlmH

Synonym: CC_3433

Alternate gene names: 16127663

Gene position: 3676620-3677087 (Clockwise)

Preceding gene: 16127662

Following gene: 16127664

Centisome position: 91.53

GC content: 69.23

Gene sequence:

>468_bases
TTGATGAAGATCACCATCCTCACCGTCGGAAAACTCGGCCGTATGGTCGAGGCGCAGCTGGCTCTGGACTATGCGTCCCG
CGCCACAGCGTCCGGCCGCGCCCTGGCGCTGGGCCCCGTCGACATTCTCGAGGTCGAGGCCAGGAAGCCCGGCAAGGCCG
CCGAGGCCGAGGTGCTGCGCCCGCATCTGGAGGGCGCCTATGTCGTGGCGTGTGACGAGCACGGCAAGGCCTGGAAGAGC
CGCGCCTTCGCCGATCACCTGGCCCGCCTGCGCGATGACGGAAACCGCCGCGTGGTGTTCCTGATCGGCGGCGCCGACGG
TCTGGATCCCTCGATCCTGGCGGCGGCGAACGAGACCATGGCCTTCGGCGTCCAGACCTGGCCGCACGCCCTGGCCCGCG
CCATGCTGGCCGAGCAGATCTATCGCGCCGCCACCATCCTGTCGGGCTCGCCCTATCACCGCGATTAA

Upstream 100 bases:

>100_bases
ACGGCAAGGCCGTCGCTCCACGAGCGACGGCCTTTAGCTTTTTTGCTTGGCGCGTGGCGGGCGCCGAAACCGGCTTCCAC
TTTCGGCTGCCACGCTTGCC

Downstream 100 bases:

>100_bases
GGCAAGAGTTGCGGCGATGTCCCGCTCGCGACTCGTCTTGGCTTTCTGCGCCCTGATCCTGGCCGTTCCCACGGCCGTGG
TCGGCTGGCAGCGCGCCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MMKITILTVGKLGRMVEAQLALDYASRATASGRALALGPVDILEVEARKPGKAAEAEVLRPHLEGAYVVACDEHGKAWKS
RAFADHLARLRDDGNRRVVFLIGGADGLDPSILAAANETMAFGVQTWPHALARAMLAEQIYRAATILSGSPYHRD

Sequences:

>Translated_155_residues
MMKITILTVGKLGRMVEAQLALDYASRATASGRALALGPVDILEVEARKPGKAAEAEVLRPHLEGAYVVACDEHGKAWKS
RAFADHLARLRDDGNRRVVFLIGGADGLDPSILAAANETMAFGVQTWPHALARAMLAEQIYRAATILSGSPYHRD
>Mature_155_residues
MMKITILTVGKLGRMVEAQLALDYASRATASGRALALGPVDILEVEARKPGKAAEAEVLRPHLEGAYVVACDEHGKAWKS
RAFADHLARLRDDGNRRVVFLIGGADGLDPSILAAANETMAFGVQTWPHALARAMLAEQIYRAATILSGSPYHRD

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=125, Percent_Identity=36.8, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_CAUCR (Q9A2X3)

Other databases:

- EMBL:   AE005673
- PIR:   G87674
- RefSeq:   NP_422227.1
- ProteinModelPortal:   Q9A2X3
- SMR:   Q9A2X3
- GeneID:   940813
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_3433
- NMPDR:   fig|190650.1.peg.3407
- TIGR:   CC_3433
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16690; Mature: 16690

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKITILTVGKLGRMVEAQLALDYASRATASGRALALGPVDILEVEARKPGKAAEAEVLR
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCHHHHHH
PHLEGAYVVACDEHGKAWKSRAFADHLARLRDDGNRRVVFLIGGADGLDPSILAAANETM
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHEEECCCCE
AFGVQTWPHALARAMLAEQIYRAATILSGSPYHRD
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMKITILTVGKLGRMVEAQLALDYASRATASGRALALGPVDILEVEARKPGKAAEAEVLR
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCHHHHHH
PHLEGAYVVACDEHGKAWKSRAFADHLARLRDDGNRRVVFLIGGADGLDPSILAAANETM
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHEEECCCCE
AFGVQTWPHALARAMLAEQIYRAATILSGSPYHRD
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647