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The map label for this gene is yogA [H]

Identifier: 16127315

GI number: 16127315

Start: 3314438

End: 3315712

Strand: Direct

Name: yogA [H]

Synonym: CC_3085

Alternate gene names: 16127315

Gene position: 3314438-3315712 (Clockwise)

Preceding gene: 16127313

Following gene: 16127318

Centisome position: 82.51

GC content: 66.82

Gene sequence:

>1275_bases
ATGACGATTCAGACGCTTGAGACCACCGCGCTGAAGGACCTGTACGAGATCGGTGAGATCCCGCCGGCCTTCCACGTGCC
GAAAACCATGTACGCCTGGAGCATCCGCAAGGAGCGCCACGGCAAGCCGACCCAGGCCATGCAGGTCGAGGTGGTGCCCA
CCTGGGAGATCGGCGAGGACGAGGTGCTGGTGCTCGTGATGGCCGCCGGCGTCAATTACAACGGGGTCTGGGCCGCTCTG
GGCGAGCCGATCAGCCCGCTGGACGGCCACAAGCAGCCTTTCCACATCGCCGGGTCCGACGCCTCGGGCATCGTCTGGAA
GGTCGGCGCCAAGGTGAAGCGGTGGAAGCTGGGCGACGAGGTCGTCATCCACTGTAACCAGGACGACGGCGACGACGAGG
AGTGCAACGGCGGCGACCCGATGTTCTCGTCCAGCCAGCGCATCTGGGGCTACGAGACGCCGGACGGCAGCTTCGCCCAG
TTCTGCCGGGTGCAGTCGCGCCAGCTGCTGCCGCGCCCCAAGCACCTGACCTGGGAAGAGAGCGCCTGCTACACCCTGAC
CCTGGCCACCGCCTACCGCATGCTGTTCGGCCACAAGCCGCATGAGCTGAAGCCCGGCCAGAACGTGCTGGTCTGGGGCG
CCTCGGGCGGTCTTGGCGTCTTCGCCACCCAGCTGGCCGCCGTGGCCGGCGCCAACGCCATCGGCGTGGTGTCCTCCGAG
GATAAGCGCGAGTTCGTGCTGTCGATGGGCGCCAAGGCGGTGCTGAACCGGGGCGAGTTCAACTGCTGGGGCCAGCTGCC
GAAGGTCAACGGCCCCGAGTTCAACGACTACATGAAGGAGAGCCGCAAGTTCGGCAAAGCCATCTGGCAGATCACCGGAA
ACAAGGACGTCGACATGGTGTTCGAGCACCCTGGCGAGCAGACCTTCCCGGTGTCGGTGTTCCTGGTCAAGCGCGGCGGC
ATGGTGGTGATCTGCGCCGGCACGACGGGCTTCAACCTGACCATGGACGCCCGCTTCCTGTGGATGCGCCAGAAGCGCGT
GCAAGGGTCGCACTTCGCCAACCTGATGCAGGCCAGCGCCGCCAACCAGCTGGTCATCGACCGCCGCGTCGATCCCTGCC
TGTCGGAAGTCTTCCCCTGGGACCAGATCCCGGCGGCCCACGAGAAGATGCTGGCCAACCAGCACCTGCCGGGGAACATG
GCCGTGCTGGTCTGCGCCCAGCGCCCCGGCCTGCGCACCTTCGAGGAAGTGCAGGAGCTGAGCGGGGCGCCATAG

Upstream 100 bases:

>100_bases
ATCGCGTTTCGACCGACGGGCGGCGCAGCCGAGTTTGACCTTTGCGCATTGTCTGCGACGCGGCGAGCGTTGTTGCAGCG
CGGAAGGAACGGGGAGTGAG

Downstream 100 bases:

>100_bases
GCGGCGGAGGGCTCCTCGGTCGGGACGCGAAGTCGGCTTGCCGGCAGGGGGCAAATGCCCGGCTTTGGGCGATGGTCTGA
TTTCCGCTGCCGACCCAGAG

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTIQTLETTALKDLYEIGEIPPAFHVPKTMYAWSIRKERHGKPTQAMQVEVVPTWEIGEDEVLVLVMAAGVNYNGVWAAL
GEPISPLDGHKQPFHIAGSDASGIVWKVGAKVKRWKLGDEVVIHCNQDDGDDEECNGGDPMFSSSQRIWGYETPDGSFAQ
FCRVQSRQLLPRPKHLTWEESACYTLTLATAYRMLFGHKPHELKPGQNVLVWGASGGLGVFATQLAAVAGANAIGVVSSE
DKREFVLSMGAKAVLNRGEFNCWGQLPKVNGPEFNDYMKESRKFGKAIWQITGNKDVDMVFEHPGEQTFPVSVFLVKRGG
MVVICAGTTGFNLTMDARFLWMRQKRVQGSHFANLMQASAANQLVIDRRVDPCLSEVFPWDQIPAAHEKMLANQHLPGNM
AVLVCAQRPGLRTFEEVQELSGAP

Sequences:

>Translated_424_residues
MTIQTLETTALKDLYEIGEIPPAFHVPKTMYAWSIRKERHGKPTQAMQVEVVPTWEIGEDEVLVLVMAAGVNYNGVWAAL
GEPISPLDGHKQPFHIAGSDASGIVWKVGAKVKRWKLGDEVVIHCNQDDGDDEECNGGDPMFSSSQRIWGYETPDGSFAQ
FCRVQSRQLLPRPKHLTWEESACYTLTLATAYRMLFGHKPHELKPGQNVLVWGASGGLGVFATQLAAVAGANAIGVVSSE
DKREFVLSMGAKAVLNRGEFNCWGQLPKVNGPEFNDYMKESRKFGKAIWQITGNKDVDMVFEHPGEQTFPVSVFLVKRGG
MVVICAGTTGFNLTMDARFLWMRQKRVQGSHFANLMQASAANQLVIDRRVDPCLSEVFPWDQIPAAHEKMLANQHLPGNM
AVLVCAQRPGLRTFEEVQELSGAP
>Mature_423_residues
TIQTLETTALKDLYEIGEIPPAFHVPKTMYAWSIRKERHGKPTQAMQVEVVPTWEIGEDEVLVLVMAAGVNYNGVWAALG
EPISPLDGHKQPFHIAGSDASGIVWKVGAKVKRWKLGDEVVIHCNQDDGDDEECNGGDPMFSSSQRIWGYETPDGSFAQF
CRVQSRQLLPRPKHLTWEESACYTLTLATAYRMLFGHKPHELKPGQNVLVWGASGGLGVFATQLAAVAGANAIGVVSSED
KREFVLSMGAKAVLNRGEFNCWGQLPKVNGPEFNDYMKESRKFGKAIWQITGNKDVDMVFEHPGEQTFPVSVFLVKRGGM
VVICAGTTGFNLTMDARFLWMRQKRVQGSHFANLMQASAANQLVIDRRVDPCLSEVFPWDQIPAAHEKMLANQHLPGNMA
VLVCAQRPGLRTFEEVQELSGAP

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=358, Percent_Identity=24.3016759776536, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI13236495, Length=358, Percent_Identity=24.3016759776536, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI194239676, Length=247, Percent_Identity=29.1497975708502, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI47519420, Length=394, Percent_Identity=24.6192893401015, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790485, Length=372, Percent_Identity=26.0752688172043, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17507255, Length=387, Percent_Identity=25.3229974160207, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=237, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=287, Percent_Identity=25.0871080139373, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323729, Length=232, Percent_Identity=29.7413793103448, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=361, Percent_Identity=25.207756232687, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=370, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=201, Percent_Identity=30.3482587064677, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 46911; Mature: 46780

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQTLETTALKDLYEIGEIPPAFHVPKTMYAWSIRKERHGKPTQAMQVEVVPTWEIGED
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHCCCCCCEEEEEEEECCCCCCC
EVLVLVMAAGVNYNGVWAALGEPISPLDGHKQPFHIAGSDASGIVWKVGAKVKRWKLGDE
CEEEEEEECCCCCCCEEHCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCE
VVIHCNQDDGDDEECNGGDPMFSSSQRIWGYETPDGSFAQFCRVQSRQLLPRPKHLTWEE
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
SACYTLTLATAYRMLFGHKPHELKPGQNVLVWGASGGLGVFATQLAAVAGANAIGVVSSE
CCEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCC
DKREFVLSMGAKAVLNRGEFNCWGQLPKVNGPEFNDYMKESRKFGKAIWQITGNKDVDMV
HHHHHHHHHCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEE
FEHPGEQTFPVSVFLVKRGGMVVICAGTTGFNLTMDARFLWMRQKRVQGSHFANLMQASA
EECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHCCHHHHHHHHHHHC
ANQLVIDRRVDPCLSEVFPWDQIPAAHEKMLANQHLPGNMAVLVCAQRPGLRTFEEVQEL
CCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHH
SGAP
CCCC
>Mature Secondary Structure 
TIQTLETTALKDLYEIGEIPPAFHVPKTMYAWSIRKERHGKPTQAMQVEVVPTWEIGED
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHCCCCCCEEEEEEEECCCCCCC
EVLVLVMAAGVNYNGVWAALGEPISPLDGHKQPFHIAGSDASGIVWKVGAKVKRWKLGDE
CEEEEEEECCCCCCCEEHCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCE
VVIHCNQDDGDDEECNGGDPMFSSSQRIWGYETPDGSFAQFCRVQSRQLLPRPKHLTWEE
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
SACYTLTLATAYRMLFGHKPHELKPGQNVLVWGASGGLGVFATQLAAVAGANAIGVVSSE
CCEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCC
DKREFVLSMGAKAVLNRGEFNCWGQLPKVNGPEFNDYMKESRKFGKAIWQITGNKDVDMV
HHHHHHHHHCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEE
FEHPGEQTFPVSVFLVKRGGMVVICAGTTGFNLTMDARFLWMRQKRVQGSHFANLMQASA
EECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHCCHHHHHHHHHHHC
ANQLVIDRRVDPCLSEVFPWDQIPAAHEKMLANQHLPGNMAVLVCAQRPGLRTFEEVQEL
CCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHH
SGAP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]