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The map label for this gene is fadH [C]

Identifier: 16127313

GI number: 16127313

Start: 3312166

End: 3313443

Strand: Direct

Name: fadH [C]

Synonym: CC_3083

Alternate gene names: 16127313

Gene position: 3312166-3313443 (Clockwise)

Preceding gene: 16127308

Following gene: 16127315

Centisome position: 82.45

GC content: 70.58

Gene sequence:

>1278_bases
ATGCTCACCGCCGCCCACGAGCCGCTGTTCGCACCCCTGGTCCTGCCCAATGGCGCCGTATTGCCGAACCGCATCGCCAA
GGCGGCGATGGAGGAGAACCTCGCCGACCCGGGCCAGCTGCCTGGGCCCGCGCTGTGGACCCTCTACCGTCGCTGGGCCC
AGGGCGGCGTCGGCTTGCAGATCACCGGTAATGTGATGGTCGATCCCTCGGCCGTGACCGGACCCGGCGGCGTGGTGCTG
GACGCCCGTGCGCCGCTCGCGCCGTTCGAGACCTGGGCGAAGGAAGCCAAGTCGGGCGGCGGCCAGGTGTGGATGCAGAT
CAATCATCCCGGCCGCCAGACCTATGCCGATCTTGGCCAGGGCGCCGTCGCCCCCTCGGCCGTGCCCGTCGACCTGGGCA
AGCTGTCGAACCTGCTCGCTCAGCCGCGCGCCCTGACGAGCGACGAGATCGTCGCCCTGGTGGAGCGCTACGCCGTCACC
GCGCGGCGCGCCGAGGACGCGGGCTTCGACGGCGTGCAGATCCACGCCGCCCACGGCTATCTGCTGAGCCAGTTCCTTTC
GCCCCTGGTCAACCGCCGCGACGACGCTTGGGGCGGCTCGCGCGAGAACCGCGCGCGGATGCTGTTTGAGGTGATCAAGG
CCGTGAGAGCGCGCGTGTCGCCGCGCTTCGTGGTCTCGGTGAAGCTGAACTCCGCCGATTTCCAGAAGGGCGGCTTCGAC
GCCGAAGACGCCCGCTGGGTCGTCGAACAGATGAACGGCATGGGCGTGGACCTGGTCGAGCTGTCGGGGGGCAACTACGA
GAACCCGGCCATGCAGGGCCCGACCAAGGCCGACGCGATCGCCCAGACCTCCACCACCGCACGCGAGGCCTATTTCGTCG
ACTTCGCCCGCGACATCGCCAAGGTCGCCAAGATGCCGATCATGGTCACCGGCGGCGTCTGCCGGCGCGCGGTGGCCCTG
GCCGCCCTGTCGCCGGAGGGCGCCCTGCCGGGCGTGCCGGTGATCGGCATCGCCCGGGCCCTGGCCTTCGCACCGGACCT
GCCCAACGCCTGGCGCAAGCCCGAGGGCCAGGACGTCGTGCTGCCGGAAGTGACCTGGAAGAACCGCTCGCTGGCCGGTC
TGGCGGTCATGGCCCTGACCAAGGCGCAGTTGAACCGCCTGGCGGCGGGCAAGGCCCCGGCCCGCAAGCTCTCGCCGCTG
CTGACCGTGATCAGCCAACGGATCAAGCAAAGCGCCCAGACCAAGCGCTATCGCGCCTGGCGCCTCAGCAACCCCTGA

Upstream 100 bases:

>100_bases
CTCGCCGATCTTCATTCCAATGCCCCCGACAGGCCCTTGACCTTCAACCTAGCTTGAAGGTGTAGAAGGTGGGACCCGCC
TGAACCGAGGTCAAGATTTC

Downstream 100 bases:

>100_bases
ACACCGTCGGCCGCTTGGCGATGAACGCCGCCCGCGCCTCGGCGTGATCGGCCGACGCCGCTAGCCCCGGGATCTCCGCC
GCCGTCGCTGACATCCCAGA

Product: FMN oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLTAAHEPLFAPLVLPNGAVLPNRIAKAAMEENLADPGQLPGPALWTLYRRWAQGGVGLQITGNVMVDPSAVTGPGGVVL
DARAPLAPFETWAKEAKSGGGQVWMQINHPGRQTYADLGQGAVAPSAVPVDLGKLSNLLAQPRALTSDEIVALVERYAVT
ARRAEDAGFDGVQIHAAHGYLLSQFLSPLVNRRDDAWGGSRENRARMLFEVIKAVRARVSPRFVVSVKLNSADFQKGGFD
AEDARWVVEQMNGMGVDLVELSGGNYENPAMQGPTKADAIAQTSTTAREAYFVDFARDIAKVAKMPIMVTGGVCRRAVAL
AALSPEGALPGVPVIGIARALAFAPDLPNAWRKPEGQDVVLPEVTWKNRSLAGLAVMALTKAQLNRLAAGKAPARKLSPL
LTVISQRIKQSAQTKRYRAWRLSNP

Sequences:

>Translated_425_residues
MLTAAHEPLFAPLVLPNGAVLPNRIAKAAMEENLADPGQLPGPALWTLYRRWAQGGVGLQITGNVMVDPSAVTGPGGVVL
DARAPLAPFETWAKEAKSGGGQVWMQINHPGRQTYADLGQGAVAPSAVPVDLGKLSNLLAQPRALTSDEIVALVERYAVT
ARRAEDAGFDGVQIHAAHGYLLSQFLSPLVNRRDDAWGGSRENRARMLFEVIKAVRARVSPRFVVSVKLNSADFQKGGFD
AEDARWVVEQMNGMGVDLVELSGGNYENPAMQGPTKADAIAQTSTTAREAYFVDFARDIAKVAKMPIMVTGGVCRRAVAL
AALSPEGALPGVPVIGIARALAFAPDLPNAWRKPEGQDVVLPEVTWKNRSLAGLAVMALTKAQLNRLAAGKAPARKLSPL
LTVISQRIKQSAQTKRYRAWRLSNP
>Mature_425_residues
MLTAAHEPLFAPLVLPNGAVLPNRIAKAAMEENLADPGQLPGPALWTLYRRWAQGGVGLQITGNVMVDPSAVTGPGGVVL
DARAPLAPFETWAKEAKSGGGQVWMQINHPGRQTYADLGQGAVAPSAVPVDLGKLSNLLAQPRALTSDEIVALVERYAVT
ARRAEDAGFDGVQIHAAHGYLLSQFLSPLVNRRDDAWGGSRENRARMLFEVIKAVRARVSPRFVVSVKLNSADFQKGGFD
AEDARWVVEQMNGMGVDLVELSGGNYENPAMQGPTKADAIAQTSTTAREAYFVDFARDIAKVAKMPIMVTGGVCRRAVAL
AALSPEGALPGVPVIGIARALAFAPDLPNAWRKPEGQDVVLPEVTWKNRSLAGLAVMALTKAQLNRLAAGKAPARKLSPL
LTVISQRIKQSAQTKRYRAWRLSNP

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=241, Percent_Identity=33.195020746888, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787939, Length=281, Percent_Identity=32.0284697508897, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559802, Length=361, Percent_Identity=35.4570637119114, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17540738, Length=360, Percent_Identity=34.1666666666667, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=35.8974358974359, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17565138, Length=350, Percent_Identity=34.8571428571429, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17559804, Length=351, Percent_Identity=34.7578347578348, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=34, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17566914, Length=354, Percent_Identity=35.8757062146893, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17566916, Length=357, Percent_Identity=30.812324929972, Blast_Score=138, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321973, Length=82, Percent_Identity=48.780487804878, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=82, Percent_Identity=45.1219512195122, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45450; Mature: 45450

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAAHEPLFAPLVLPNGAVLPNRIAKAAMEENLADPGQLPGPALWTLYRRWAQGGVGLQ
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
ITGNVMVDPSAVTGPGGVVLDARAPLAPFETWAKEAKSGGGQVWMQINHPGRQTYADLGQ
EECCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCC
GAVAPSAVPVDLGKLSNLLAQPRALTSDEIVALVERYAVTARRAEDAGFDGVQIHAAHGY
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
LLSQFLSPLVNRRDDAWGGSRENRARMLFEVIKAVRARVSPRFVVSVKLNSADFQKGGFD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
AEDARWVVEQMNGMGVDLVELSGGNYENPAMQGPTKADAIAQTSTTAREAYFVDFARDIA
CHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
KVAKMPIMVTGGVCRRAVALAALSPEGALPGVPVIGIARALAFAPDLPNAWRKPEGQDVV
HHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEE
LPEVTWKNRSLAGLAVMALTKAQLNRLAAGKAPARKLSPLLTVISQRIKQSAQTKRYRAW
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
RLSNP
ECCCC
>Mature Secondary Structure
MLTAAHEPLFAPLVLPNGAVLPNRIAKAAMEENLADPGQLPGPALWTLYRRWAQGGVGLQ
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
ITGNVMVDPSAVTGPGGVVLDARAPLAPFETWAKEAKSGGGQVWMQINHPGRQTYADLGQ
EECCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCC
GAVAPSAVPVDLGKLSNLLAQPRALTSDEIVALVERYAVTARRAEDAGFDGVQIHAAHGY
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
LLSQFLSPLVNRRDDAWGGSRENRARMLFEVIKAVRARVSPRFVVSVKLNSADFQKGGFD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
AEDARWVVEQMNGMGVDLVELSGGNYENPAMQGPTKADAIAQTSTTAREAYFVDFARDIA
CHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
KVAKMPIMVTGGVCRRAVALAALSPEGALPGVPVIGIARALAFAPDLPNAWRKPEGQDVV
HHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEE
LPEVTWKNRSLAGLAVMALTKAQLNRLAAGKAPARKLSPLLTVISQRIKQSAQTKRYRAW
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
RLSNP
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]