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The map label for this gene is purR [H]

Identifier: 16127295

GI number: 16127295

Start: 3290714

End: 3291769

Strand: Direct

Name: purR [H]

Synonym: CC_3065

Alternate gene names: 16127295

Gene position: 3290714-3291769 (Clockwise)

Preceding gene: 16127293

Following gene: 16127298

Centisome position: 81.92

GC content: 70.83

Gene sequence:

>1056_bases
ATGAACCAGCCTGTCGAGCGCCAGCGTCGCCGGACGACCCAGAGCGCCACGATCCGCGACGTGGCGGCCCGGGCGGGGGT
CTCGCCGATGACGGTGTCGCGGGTCATCAATCGCGAGAGCACGGTCAAGGAAGAGACCCGCCAGCTGGTCGAGAAGGCCA
TCGCCGACCTCAACTACGCGCCCAATCCCGCCGCCCGCAGCCTGGCCGGCAGCGCGCCGTTCCGCATCGGCCTGCTCTAC
GACAACCCCTCGACGGGCTACCTGTCGGAGTTCCTGGTCGGGGCGCTGGACGAAAGCAGCCGCACCGGGGCCCAGATCGT
CATCGAAAAGTGCGCCGAGCCGGAGCTGGCCCGCGCCACCCTGGCCCGCCTGCTGAAGACCGGCGTCGATGGCCTGATCC
TGCCGCCGCCACTGTGCGAGTCTCCCGAGGTGCTGGCCGAGATCCGCGCGGCGGGCGCCGCCGCCGTGGCCGTGGCGCCC
GGCACGGCCAGCGCCGACATGGCCACCATCCGCATCGACAACGAGGCGGCCGCCTTCGAGCTGACCCAGCACCTGATCGG
CCTTGGCCACAAGCGCTTCGGCTTCATCAAGGGCCATCCGAACCAGACGGTCAGCCAGCAGCGCCTGGACGGCTTCATGA
CCGCCCTGAAGGCCGCCGGAATTCCGCAGGAAAACATCCGGGTCGAGCAGGGCTATTTCACCTATCGCTCGGGCCTGGAG
GCGGCCGAGCGCCTGCTGGCCGCCGAGCCGCGTCCCACCGCGATCTTTGCGGCCAATGACGACATGGCCGCCGCCACGGC
GGGCGTGGCCCATCGCCTGGGCCTGGACGTGCCGGGCGACGTCTCGATCGTCGGCTTCGACGACACCTCGATCGCCGACA
ACATCTGGCCGCCCCTGACCACGGTCCATCAGCCGATCGCGGCCATGGCCCGCGCGGCCGTCGACCTGGTCCTGGAAGAA
ATCCGCCGCCATCGCGACGGCGGCGGCGAACCGCGCCAGCTGATGCACCCGCATACCCTGATCGTGCGGGATTCCAGCGG
GCCGGCGGGGGTCTAG

Upstream 100 bases:

>100_bases
CAGGGAAGTCATGAGCCCGGTTTAGCAGGAAAACCCGCTGCTCTTCCAAGCCGTACCCGAAGCGGTCTAGTGTCCGCGCC
GAAGAGGGACGGACTCAAGA

Downstream 100 bases:

>100_bases
CCGCTTAAAGAAATCCCTCTCTCATGGAGAGAGGGAGGGGCCCGCCGCGAAGCGGTGGGAGGGTGAGTGGGTACGTTCTT
TCCGGACAAGGCAGGGACGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNQPVERQRRRTTQSATIRDVAARAGVSPMTVSRVINRESTVKEETRQLVEKAIADLNYAPNPAARSLAGSAPFRIGLLY
DNPSTGYLSEFLVGALDESSRTGAQIVIEKCAEPELARATLARLLKTGVDGLILPPPLCESPEVLAEIRAAGAAAVAVAP
GTASADMATIRIDNEAAAFELTQHLIGLGHKRFGFIKGHPNQTVSQQRLDGFMTALKAAGIPQENIRVEQGYFTYRSGLE
AAERLLAAEPRPTAIFAANDDMAAATAGVAHRLGLDVPGDVSIVGFDDTSIADNIWPPLTTVHQPIAAMARAAVDLVLEE
IRRHRDGGGEPRQLMHPHTLIVRDSSGPAGV

Sequences:

>Translated_351_residues
MNQPVERQRRRTTQSATIRDVAARAGVSPMTVSRVINRESTVKEETRQLVEKAIADLNYAPNPAARSLAGSAPFRIGLLY
DNPSTGYLSEFLVGALDESSRTGAQIVIEKCAEPELARATLARLLKTGVDGLILPPPLCESPEVLAEIRAAGAAAVAVAP
GTASADMATIRIDNEAAAFELTQHLIGLGHKRFGFIKGHPNQTVSQQRLDGFMTALKAAGIPQENIRVEQGYFTYRSGLE
AAERLLAAEPRPTAIFAANDDMAAATAGVAHRLGLDVPGDVSIVGFDDTSIADNIWPPLTTVHQPIAAMARAAVDLVLEE
IRRHRDGGGEPRQLMHPHTLIVRDSSGPAGV
>Mature_351_residues
MNQPVERQRRRTTQSATIRDVAARAGVSPMTVSRVINRESTVKEETRQLVEKAIADLNYAPNPAARSLAGSAPFRIGLLY
DNPSTGYLSEFLVGALDESSRTGAQIVIEKCAEPELARATLARLLKTGVDGLILPPPLCESPEVLAEIRAAGAAAVAVAP
GTASADMATIRIDNEAAAFELTQHLIGLGHKRFGFIKGHPNQTVSQQRLDGFMTALKAAGIPQENIRVEQGYFTYRSGLE
AAERLLAAEPRPTAIFAANDDMAAATAGVAHRLGLDVPGDVSIVGFDDTSIADNIWPPLTTVHQPIAAMARAAVDLVLEE
IRRHRDGGGEPRQLMHPHTLIVRDSSGPAGV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=36.3636363636364, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=33.5463258785943, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=32.0588235294118, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=30.2395209580838, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786540, Length=305, Percent_Identity=30.8196721311475, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=31.7567567567568, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=33.7662337662338, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=29.2207792207792, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=25.6329113924051, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=24.024024024024, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=27.7456647398844, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=27.7456647398844, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=24.8502994011976, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786268, Length=308, Percent_Identity=25.6493506493506, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37438; Mature: 37438

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPVERQRRRTTQSATIRDVAARAGVSPMTVSRVINRESTVKEETRQLVEKAIADLNYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PNPAARSLAGSAPFRIGLLYDNPSTGYLSEFLVGALDESSRTGAQIVIEKCAEPELARAT
CCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
LARLLKTGVDGLILPPPLCESPEVLAEIRAAGAAAVAVAPGTASADMATIRIDNEAAAFE
HHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCHHHHH
LTQHLIGLGHKRFGFIKGHPNQTVSQQRLDGFMTALKAAGIPQENIRVEQGYFTYRSGLE
HHHHHHHCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCEEECCHHHHHHHHH
AAERLLAAEPRPTAIFAANDDMAAATAGVAHRLGLDVPGDVSIVGFDDTSIADNIWPPLT
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHCCCCCHH
TVHQPIAAMARAAVDLVLEEIRRHRDGGGEPRQLMHPHTLIVRDSSGPAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MNQPVERQRRRTTQSATIRDVAARAGVSPMTVSRVINRESTVKEETRQLVEKAIADLNYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PNPAARSLAGSAPFRIGLLYDNPSTGYLSEFLVGALDESSRTGAQIVIEKCAEPELARAT
CCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
LARLLKTGVDGLILPPPLCESPEVLAEIRAAGAAAVAVAPGTASADMATIRIDNEAAAFE
HHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCHHHHH
LTQHLIGLGHKRFGFIKGHPNQTVSQQRLDGFMTALKAAGIPQENIRVEQGYFTYRSGLE
HHHHHHHCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCEEECCHHHHHHHHH
AAERLLAAEPRPTAIFAANDDMAAATAGVAHRLGLDVPGDVSIVGFDDTSIADNIWPPLT
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHCCCCCHH
TVHQPIAAMARAAVDLVLEEIRRHRDGGGEPRQLMHPHTLIVRDSSGPAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA