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The map label for this gene is apbE [H]

Identifier: 16127292

GI number: 16127292

Start: 3287450

End: 3288478

Strand: Direct

Name: apbE [H]

Synonym: CC_3062

Alternate gene names: 16127292

Gene position: 3287450-3288478 (Clockwise)

Preceding gene: 16127291

Following gene: 16127293

Centisome position: 81.84

GC content: 72.98

Gene sequence:

>1029_bases
ATGACACGGCTTTTGTTGTTGCAAAGCATGCCCCGCGTCCTCGTCCCCGCCATCGCCACGCCGCCCGCCCGCCCCGTGGG
CGGGAGCGTCCGCGCGTTCGGCGGCGAGACCATGGGCACGACCTGGTCGGTCAAGGCGGTGCTGCCGGCCACGACCGACC
TGGCCGCCCTGAAGGCGAGCGTCGAGCGCGCGCTGGACGCGGTCGTTTCCCAGATGAGCCCCTGGGCGCCGCTGTCGGAC
CTCTCCCGCTACAATCGCGCCGCGCCGGGATCCTGGACTGTGCTGCCGCCGGACACACTGACCGTGCTGCGCCGAGCCCT
CGACGTCGCCGCGCAGACCGACGGCGCGTTCGATCCGACCCTGGGCGCCCTGACCGACCTCTGGGGCTTTGGCCCGCGTC
CGTTCGCCGGCCAGCCGCCGCAAGCCGAGACCCTGGCCCCGCGCCGCGAGGCCTGCGGCTGGAAGCGCCTGGTGCTTGAC
GGCGAGGCGCTGTTCCAGCCGGGCGGGCTGAAGCTTGATCTGAACGGCATCGCCAAGGGCTTTGGCGTCGACCAGGCCGC
CGCCGTGCTCGATCGCGCCGGGGTCAGGAGCTACCTCATCGAGGTCGGCGGCGAGCTGCGCGGGATCGGCGCCAAGCCCG
ACGGCCAGCCCTGGTGGGTCGAGCTGGAGCGCCCGCCGCCCTTCGAGCCCGCCGCCAACGACACCCAGAAAACCCTCGTG
GCCCTGCATGACCTGGCCGTCGCCACCTCGGGCGACTATCGCCGGTTCTTCGACCATGGCGGACGGCGCTACGCCCACAC
GCTGGACCCGGCCACCGGCGCGCCGATCGTCCACGCCACCGTCAGCGTCACGGTACTGGCCAAGGACTGCATCAGCGCCG
ACGCCTGGGCCACGGCCCTGACCGTCATGCCGCCCGAGCGCGCCCTGGCCTTCGCCGCCGCCCACGATCTGGCCGCCCTG
ATCGTCAGCCGAGGCCCGTCCGGTCTTGAGGAACGGCTGTCGCCCGCCCTGCAGGCGATGCTGGACTGA

Upstream 100 bases:

>100_bases
AACTGTCATCCCGGCCCAGCGCGCAGCGCGCCGAGCCGGGACCCAGGGGCCGACGCACCGTCGCTAGCCCCTGGGTCCCG
GGTCGACTTCGTCGCCCGGG

Downstream 100 bases:

>100_bases
TGCCCGACCTCGCCTTCCTCCCGCCTGAGGCCCGGCGTTGGGTCTGGGCAGGCGTCGTCGTGGCGCTGTACGCCCTGTTC
TGCCTGGCCATCGCCCTGCG

Product: thiamine biosynthesis protein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTRLLLLQSMPRVLVPAIATPPARPVGGSVRAFGGETMGTTWSVKAVLPATTDLAALKASVERALDAVVSQMSPWAPLSD
LSRYNRAAPGSWTVLPPDTLTVLRRALDVAAQTDGAFDPTLGALTDLWGFGPRPFAGQPPQAETLAPRREACGWKRLVLD
GEALFQPGGLKLDLNGIAKGFGVDQAAAVLDRAGVRSYLIEVGGELRGIGAKPDGQPWWVELERPPPFEPAANDTQKTLV
ALHDLAVATSGDYRRFFDHGGRRYAHTLDPATGAPIVHATVSVTVLAKDCISADAWATALTVMPPERALAFAAAHDLAAL
IVSRGPSGLEERLSPALQAMLD

Sequences:

>Translated_342_residues
MTRLLLLQSMPRVLVPAIATPPARPVGGSVRAFGGETMGTTWSVKAVLPATTDLAALKASVERALDAVVSQMSPWAPLSD
LSRYNRAAPGSWTVLPPDTLTVLRRALDVAAQTDGAFDPTLGALTDLWGFGPRPFAGQPPQAETLAPRREACGWKRLVLD
GEALFQPGGLKLDLNGIAKGFGVDQAAAVLDRAGVRSYLIEVGGELRGIGAKPDGQPWWVELERPPPFEPAANDTQKTLV
ALHDLAVATSGDYRRFFDHGGRRYAHTLDPATGAPIVHATVSVTVLAKDCISADAWATALTVMPPERALAFAAAHDLAAL
IVSRGPSGLEERLSPALQAMLD
>Mature_341_residues
TRLLLLQSMPRVLVPAIATPPARPVGGSVRAFGGETMGTTWSVKAVLPATTDLAALKASVERALDAVVSQMSPWAPLSDL
SRYNRAAPGSWTVLPPDTLTVLRRALDVAAQTDGAFDPTLGALTDLWGFGPRPFAGQPPQAETLAPRREACGWKRLVLDG
EALFQPGGLKLDLNGIAKGFGVDQAAAVLDRAGVRSYLIEVGGELRGIGAKPDGQPWWVELERPPPFEPAANDTQKTLVA
LHDLAVATSGDYRRFFDHGGRRYAHTLDPATGAPIVHATVSVTVLAKDCISADAWATALTVMPPERALAFAAAHDLAALI
VSRGPSGLEERLSPALQAMLD

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=328, Percent_Identity=32.0121951219512, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 36153; Mature: 36022

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLLLQSMPRVLVPAIATPPARPVGGSVRAFGGETMGTTWSVKAVLPATTDLAALKAS
CCHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHH
VERALDAVVSQMSPWAPLSDLSRYNRAAPGSWTVLPPDTLTVLRRALDVAAQTDGAFDPT
HHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCH
LGALTDLWGFGPRPFAGQPPQAETLAPRREACGWKRLVLDGEALFQPGGLKLDLNGIAKG
HHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHCCCEEEEECCHHHCCCCCCEEEHHHHHCC
FGVDQAAAVLDRAGVRSYLIEVGGELRGIGAKPDGQPWWVELERPPPFEPAANDTQKTLV
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHH
ALHDLAVATSGDYRRFFDHGGRRYAHTLDPATGAPIVHATVSVTVLAKDCISADAWATAL
HHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEHHHHHHCCHHHHHEE
TVMPPERALAFAAAHDLAALIVSRGPSGLEERLSPALQAMLD
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRLLLLQSMPRVLVPAIATPPARPVGGSVRAFGGETMGTTWSVKAVLPATTDLAALKAS
CHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHH
VERALDAVVSQMSPWAPLSDLSRYNRAAPGSWTVLPPDTLTVLRRALDVAAQTDGAFDPT
HHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCH
LGALTDLWGFGPRPFAGQPPQAETLAPRREACGWKRLVLDGEALFQPGGLKLDLNGIAKG
HHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHCCCEEEEECCHHHCCCCCCEEEHHHHHCC
FGVDQAAAVLDRAGVRSYLIEVGGELRGIGAKPDGQPWWVELERPPPFEPAANDTQKTLV
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHH
ALHDLAVATSGDYRRFFDHGGRRYAHTLDPATGAPIVHATVSVTVLAKDCISADAWATAL
HHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEHHHHHHCCHHHHHEE
TVMPPERALAFAAAHDLAALIVSRGPSGLEERLSPALQAMLD
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]