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The map label for this gene is rspA [H]

Identifier: 16127044

GI number: 16127044

Start: 3032991

End: 3034202

Strand: Direct

Name: rspA [H]

Synonym: CC_2812

Alternate gene names: 16127044

Gene position: 3032991-3034202 (Clockwise)

Preceding gene: 16127040

Following gene: 16127045

Centisome position: 75.5

GC content: 67.41

Gene sequence:

>1212_bases
ATGCCCAAGATCATCGCCGCCAAGACCATCGTCACCTGCCCGGGGCGCAACTTCGTCACCCTGAAGATCATGACCGACGA
GGGCGTCTACGGCCTGGGCGACGCCACGCTGAACGGCCGCGAGCTGGCGGTCGAGGCCTATCTGACCCAGCACGTCATCC
CCTGCCTGATCGGCCGTGACGCCCACCAGATCGAGGACATCTGGCAGTACCTGTATCGCGGCTGCTACTGGCGTCGGGGC
CCGGTGACCATGGCCGCCATCGCCGCCGTCGACACCGCCCTGTGGGACATCAAGGGCAAGATCGCCGGCCTGCCGGTCTA
TCAGCTCCTGGGCGGCGCCTGCCGGGTCGGCGTCATGGTCTATGGCCACGCCAATGGCGAGACGATCGACGAGACCCTCG
ACAACGCCGCCGTCTACGCCCAGCAGGGCTACAAGGCCATCCGCCTGCAGACCGGCGTACCCGGCATGAGCGGGACCTAT
GGCGTCTCCAAGGACAAGTTCTTCTATGAGCCGGCCGACAGCGACCTGCCCAAGGAGACGATCTGGTCGACCGAGCGCTA
TCTGCGCAGCACCCCCGCCCTGTTCGAGGCCGCGCGCGAGCGGCTGGGCGATGACCTGCACCTGCTGCACGACGTCCACC
ACCGCCTGACCCCGATCGAGGCCGCGCGCCTGGGCAAGGACCTGGAGCCCTACCGCCTGTTCTGGATGGAGGACGCCACC
CCGGCCGAGAACCAGGCCAGTTTCCGCCTGATCCGCCAGCACACCACGACCCCGCTGGCCGTCGGCGAGATCTTCAACTC
GATCTGGGACTGCAAGCAGCTGATCGAGGAGCAGTTGATCGACTACATCCGCGCCACGGTCGTTCACGCCGGCGGGATCA
CCCACCTGAAGAAGCTGGCCAGCTTCGCCGACCTGCACCATGTGCGCACCGGCTGCCACGGCGCCACGGATCTTTCGCCG
GTCTGCATGGGCGCGGCCCTGCACTTTGACCTGTCGATCCCCAACTTCGGCGTCCAGGAATACATGCGCCACACGCCCGA
GACGGACGCGGTGTTCCCCCACGCCTACACCTTCAAGGACGGCATGCTGCACCCCGGCGACGCCCCGGGCCTGGGCGTCG
ACATCGACGAGGACCTGGCGGCGAAGTACCCCTATCAGCGCGCCTATCTGCCCATCGCCCGCCGCCTCGACGGGTCGATG
CACGACTGGTGA

Upstream 100 bases:

>100_bases
GATAATCGGGAGGCGGTTGGAGCGCTGCGCGACAAGGCGCTGGCTTTGGCTTTTGACCTCCGGCTCAGTCCAGCCTCTGC
ACAAGGTCGAAGCGTCCTCC

Downstream 100 bases:

>100_bases
GCGTGGTCTGACCTCCGCGACAATGCGCGCGAAAAATGAGGCTCATCGGCGCGGTTGGCCGTGATAGAGATTGGCGCTTT
CCCCCGCCCTGGGGCGTGTT

Product: mandelate racemase/muconate lactonizing family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPKIIAAKTIVTCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVEAYLTQHVIPCLIGRDAHQIEDIWQYLYRGCYWRRG
PVTMAAIAAVDTALWDIKGKIAGLPVYQLLGGACRVGVMVYGHANGETIDETLDNAAVYAQQGYKAIRLQTGVPGMSGTY
GVSKDKFFYEPADSDLPKETIWSTERYLRSTPALFEAARERLGDDLHLLHDVHHRLTPIEAARLGKDLEPYRLFWMEDAT
PAENQASFRLIRQHTTTPLAVGEIFNSIWDCKQLIEEQLIDYIRATVVHAGGITHLKKLASFADLHHVRTGCHGATDLSP
VCMGAALHFDLSIPNFGVQEYMRHTPETDAVFPHAYTFKDGMLHPGDAPGLGVDIDEDLAAKYPYQRAYLPIARRLDGSM
HDW

Sequences:

>Translated_403_residues
MPKIIAAKTIVTCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVEAYLTQHVIPCLIGRDAHQIEDIWQYLYRGCYWRRG
PVTMAAIAAVDTALWDIKGKIAGLPVYQLLGGACRVGVMVYGHANGETIDETLDNAAVYAQQGYKAIRLQTGVPGMSGTY
GVSKDKFFYEPADSDLPKETIWSTERYLRSTPALFEAARERLGDDLHLLHDVHHRLTPIEAARLGKDLEPYRLFWMEDAT
PAENQASFRLIRQHTTTPLAVGEIFNSIWDCKQLIEEQLIDYIRATVVHAGGITHLKKLASFADLHHVRTGCHGATDLSP
VCMGAALHFDLSIPNFGVQEYMRHTPETDAVFPHAYTFKDGMLHPGDAPGLGVDIDEDLAAKYPYQRAYLPIARRLDGSM
HDW
>Mature_402_residues
PKIIAAKTIVTCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVEAYLTQHVIPCLIGRDAHQIEDIWQYLYRGCYWRRGP
VTMAAIAAVDTALWDIKGKIAGLPVYQLLGGACRVGVMVYGHANGETIDETLDNAAVYAQQGYKAIRLQTGVPGMSGTYG
VSKDKFFYEPADSDLPKETIWSTERYLRSTPALFEAARERLGDDLHLLHDVHHRLTPIEAARLGKDLEPYRLFWMEDATP
AENQASFRLIRQHTTTPLAVGEIFNSIWDCKQLIEEQLIDYIRATVVHAGGITHLKKLASFADLHHVRTGCHGATDLSPV
CMGAALHFDLSIPNFGVQEYMRHTPETDAVFPHAYTFKDGMLHPGDAPGLGVDIDEDLAAKYPYQRAYLPIARRLDGSMH
DW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=403, Percent_Identity=66.2531017369727, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI48994953, Length=410, Percent_Identity=30.7317073170732, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI226510960, Length=322, Percent_Identity=25.776397515528, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 44894; Mature: 44763

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIIAAKTIVTCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVEAYLTQHVIPCLIGRD
CCCEEEEEEEEECCCCCEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AHQIEDIWQYLYRGCYWRRGPVTMAAIAAVDTALWDIKGKIAGLPVYQLLGGACRVGVMV
HHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHEEEEEE
YGHANGETIDETLDNAAVYAQQGYKAIRLQTGVPGMSGTYGVSKDKFFYEPADSDLPKET
EECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHH
IWSTERYLRSTPALFEAARERLGDDLHLLHDVHHRLTPIEAARLGKDLEPYRLFWMEDAT
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCC
PAENQASFRLIRQHTTTPLAVGEIFNSIWDCKQLIEEQLIDYIRATVVHAGGITHLKKLA
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFADLHHVRTGCHGATDLSPVCMGAALHFDLSIPNFGVQEYMRHTPETDAVFPHAYTFKD
HHHHHHHHHHCCCCCCCCCHHHHCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCEEECCC
GMLHPGDAPGLGVDIDEDLAAKYPYQRAYLPIARRLDGSMHDW
CCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PKIIAAKTIVTCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVEAYLTQHVIPCLIGRD
CCEEEEEEEEECCCCCEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AHQIEDIWQYLYRGCYWRRGPVTMAAIAAVDTALWDIKGKIAGLPVYQLLGGACRVGVMV
HHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHEEEEEE
YGHANGETIDETLDNAAVYAQQGYKAIRLQTGVPGMSGTYGVSKDKFFYEPADSDLPKET
EECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHH
IWSTERYLRSTPALFEAARERLGDDLHLLHDVHHRLTPIEAARLGKDLEPYRLFWMEDAT
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCC
PAENQASFRLIRQHTTTPLAVGEIFNSIWDCKQLIEEQLIDYIRATVVHAGGITHLKKLA
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFADLHHVRTGCHGATDLSPVCMGAALHFDLSIPNFGVQEYMRHTPETDAVFPHAYTFKD
HHHHHHHHHHCCCCCCCCCHHHHCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCEEECCC
GMLHPGDAPGLGVDIDEDLAAKYPYQRAYLPIARRLDGSMHDW
CCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]