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The map label for this gene is rutB [H]

Identifier: 16127027

GI number: 16127027

Start: 3007792

End: 3008469

Strand: Direct

Name: rutB [H]

Synonym: CC_2795

Alternate gene names: 16127027

Gene position: 3007792-3008469 (Clockwise)

Preceding gene: 16127023

Following gene: 16127028

Centisome position: 74.88

GC content: 67.26

Gene sequence:

>678_bases
ATGCTGCCGGCGCGGCCCGAGCCCCTGCCGGTGGACCCGAAGACCACCGCGGTGATCGTCATCGACATGCAGAACGCCTA
CGCCTCGCCCGGCGGCTATCTGGACCTGGCGGGCTTCGACATCTCGGGCGCGGCCAAGGTGACCCACGAGATCAAGGGCG
TGCTGGAGGTGGCCCGCAGCGCCGGTATGACCGTGATCTATTTCCAGAACGGCTGGGACGACGGCTACGTCGAGGCCGGC
GGTCCCGGCTCGCCCAACTGGTGGAAGTCCAACGCCCTGAAGACCATGCGCGCCCGCCCCGAGCTGCAGGGCAAGCTCTT
GGCGCGCGGCCAGTGGGACTATGAGCTGGTCGACGACCTGACGCCCCAGCCGGGCGACATCCGCCTGCACAAGACACGCT
ATTCGGGCTTCTTCAACAGCCAGCTGGACAGCGTGCTGCGGGCGCGCGGCATCCGCCATCTGGTGTTCACCGGCATCGCC
ACGAACGTGTGCGTCGAGTCGACCCTGCGCGACGGCTTCATGCTGGAGTATTTCGGAACCGTGCTGGAGGACGCCACCCA
CCAGGCGGGCCCCGACTTCGTCCAGAAGGCCGCCCTGTTCAACATCGAGACCTTCTTCGGCTGGGTGTCGACCACGGCGG
ATTTCAAGGGGACGTTCGGGCAGTTGGCGCCGGGTTGA

Upstream 100 bases:

>100_bases
GATTGCAACGTTACGCGAGATGGTATTCCATGCCTGTCGCAAATCACAAGGAGCCGCGTTGATGAGCTCTCCGATCACCC
CGCTCTCGCCTGGCTGCGTC

Downstream 100 bases:

>100_bases
GACGCCGTGCGTCCTTCGAGGCTCGCCTTAGGCTCGCACCTCAGGATGAGGAAGACTGAGCAAGCCGTACTGAACACCTC
ATCCTGAGGCGCCCGCTCCT

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLPARPEPLPVDPKTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARSAGMTVIYFQNGWDDGYVEAG
GPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDLTPQPGDIRLHKTRYSGFFNSQLDSVLRARGIRHLVFTGIA
TNVCVESTLRDGFMLEYFGTVLEDATHQAGPDFVQKAALFNIETFFGWVSTTADFKGTFGQLAPG

Sequences:

>Translated_225_residues
MLPARPEPLPVDPKTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARSAGMTVIYFQNGWDDGYVEAG
GPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDLTPQPGDIRLHKTRYSGFFNSQLDSVLRARGIRHLVFTGIA
TNVCVESTLRDGFMLEYFGTVLEDATHQAGPDFVQKAALFNIETFFGWVSTTADFKGTFGQLAPG
>Mature_225_residues
MLPARPEPLPVDPKTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARSAGMTVIYFQNGWDDGYVEAG
GPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDLTPQPGDIRLHKTRYSGFFNSQLDSVLRARGIRHLVFTGIA
TNVCVESTLRDGFMLEYFGTVLEDATHQAGPDFVQKAALFNIETFFGWVSTTADFKGTFGQLAPG

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=214, Percent_Identity=73.8317757009346, Blast_Score=335, Evalue=1e-93,
Organism=Escherichia coli, GI87081992, Length=184, Percent_Identity=28.804347826087, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24634; Mature: 24634

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPARPEPLPVDPKTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARS
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AGMTVIYFQNGWDDGYVEAGGPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDL
CCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHC
TPQPGDIRLHKTRYSGFFNSQLDSVLRARGIRHLVFTGIATNVCVESTLRDGFMLEYFGT
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VLEDATHQAGPDFVQKAALFNIETFFGWVSTTADFKGTFGQLAPG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPARPEPLPVDPKTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARS
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AGMTVIYFQNGWDDGYVEAGGPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDL
CCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHC
TPQPGDIRLHKTRYSGFFNSQLDSVLRARGIRHLVFTGIATNVCVESTLRDGFMLEYFGT
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VLEDATHQAGPDFVQKAALFNIETFFGWVSTTADFKGTFGQLAPG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA