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The map label for this gene is yjbJ [H]

Identifier: 16126655

GI number: 16126655

Start: 2622042

End: 2622698

Strand: Direct

Name: yjbJ [H]

Synonym: CC_2416

Alternate gene names: 16126655

Gene position: 2622042-2622698 (Clockwise)

Preceding gene: 16126651

Following gene: 16126656

Centisome position: 65.27

GC content: 67.28

Gene sequence:

>657_bases
ATGGAGGACACACTGGGGGAGTTGGTGCGCCGTTCTGTTCGATCCCGTTCAGCGCCTGGCCTGGCCTGGGCCTGCGGCGT
CGCCGCGATCGGCTTGCTTTGCGCCGCTCGGGGGCACGCTCAGGTCTTGGAGATCGACGACCAAGGCGCCGTTCGCCGCT
TTGATGGTCCCACCCAATTCCTAGGCGATGGTCCGCCGGTTCCCCTTCAGGCTGCTTCACGCGCCGAGCGTCATCGGATG
GGAAGCGACCGCACGCCGACCGCGCCGGTCAAACGCCTCCTCGAAGACGCAGCGCAGCGCTCACAGATCAGCCCCGACCT
GTTGACCGCCGTCGCCCGTCGCGAATCCGGACTAAACCCGCAAGCGCGGTCCGCGAAGGGCGCGCTGGGCGTCATGCAGT
TGATGCCTGGCACGGCCAAGGACCTGGCCGTCGATCCGCTTGATCCCGCCGCCAATGTCGACGGCGGAACGCGTTACCTG
AAAACACTTCTGGCCCGCTATGACGGCGACGTCATCAAGGCCCTCGCCGCTTACAACGCGGGCATGGGCGCGGTGGACCG
TCACGGCGGCGTTCCCCCGTTTCCTGAGACCAAAGCCTATGTGGACGCCATTCTCGAGCACATGGCCCAGGCCGTGGCGC
CGCGCCGCAAGCCCTGA

Upstream 100 bases:

>100_bases
CGTCGCAAGGCGAAGATCTTTTTTGTCACAGCGACGGACTTGGCTCCCGAGCGCGTGAAAAGGCTGACCTTGTGCGGCGC
GTACGAAGCGCGTGATAGTC

Downstream 100 bases:

>100_bases
TCGGAGCGCATCTTGACCCTGGTTCGTCGTCTTTCGCCGGCTTTGGCCCTCGCCGCGCTCGCCGCCCCCGCTCACGCCCA
AACGGTGCAGAGCGACCCGG

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MEDTLGELVRRSVRSRSAPGLAWACGVAAIGLLCAARGHAQVLEIDDQGAVRRFDGPTQFLGDGPPVPLQAASRAERHRM
GSDRTPTAPVKRLLEDAAQRSQISPDLLTAVARRESGLNPQARSAKGALGVMQLMPGTAKDLAVDPLDPAANVDGGTRYL
KTLLARYDGDVIKALAAYNAGMGAVDRHGGVPPFPETKAYVDAILEHMAQAVAPRRKP

Sequences:

>Translated_218_residues
MEDTLGELVRRSVRSRSAPGLAWACGVAAIGLLCAARGHAQVLEIDDQGAVRRFDGPTQFLGDGPPVPLQAASRAERHRM
GSDRTPTAPVKRLLEDAAQRSQISPDLLTAVARRESGLNPQARSAKGALGVMQLMPGTAKDLAVDPLDPAANVDGGTRYL
KTLLARYDGDVIKALAAYNAGMGAVDRHGGVPPFPETKAYVDAILEHMAQAVAPRRKP
>Mature_218_residues
MEDTLGELVRRSVRSRSAPGLAWACGVAAIGLLCAARGHAQVLEIDDQGAVRRFDGPTQFLGDGPPVPLQAASRAERHRM
GSDRTPTAPVKRLLEDAAQRSQISPDLLTAVARRESGLNPQARSAKGALGVMQLMPGTAKDLAVDPLDPAANVDGGTRYL
KTLLARYDGDVIKALAAYNAGMGAVDRHGGVPPFPETKAYVDAILEHMAQAVAPRRKP

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=122, Percent_Identity=36.0655737704918, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23060; Mature: 23060

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDTLGELVRRSVRSRSAPGLAWACGVAAIGLLCAARGHAQVLEIDDQGAVRRFDGPTQF
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
LGDGPPVPLQAASRAERHRMGSDRTPTAPVKRLLEDAAQRSQISPDLLTAVARRESGLNP
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
QARSAKGALGVMQLMPGTAKDLAVDPLDPAANVDGGTRYLKTLLARYDGDVIKALAAYNA
HHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
GMGAVDRHGGVPPFPETKAYVDAILEHMAQAVAPRRKP
CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEDTLGELVRRSVRSRSAPGLAWACGVAAIGLLCAARGHAQVLEIDDQGAVRRFDGPTQF
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
LGDGPPVPLQAASRAERHRMGSDRTPTAPVKRLLEDAAQRSQISPDLLTAVARRESGLNP
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
QARSAKGALGVMQLMPGTAKDLAVDPLDPAANVDGGTRYLKTLLARYDGDVIKALAAYNA
HHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
GMGAVDRHGGVPPFPETKAYVDAILEHMAQAVAPRRKP
CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]