Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 16126073

GI number: 16126073

Start: 2024543

End: 2026327

Strand: Direct

Name: gatA [H]

Synonym: CC_1830

Alternate gene names: 16126073

Gene position: 2024543-2026327 (Clockwise)

Preceding gene: 16126072

Following gene: 16126079

Centisome position: 50.4

GC content: 72.32

Gene sequence:

>1785_bases
ATGAACGCCCCGCGCCTCCTCGTCGGCGAGATCGCCGCCGGCGTGAACGCCGGCGACTCGGCGGAGTCCGTCGCCCGCTC
GGCCCTGGCGGCGATCGAGGCCTACGACGCGATCCAGCCGCAGGTCTGGACCTTGCGGCTGCCCGAGGCCGCCGTTCTGG
CCCAGGCCCGCGCCGTCGACGCTCGCATTGCCGCCGGCGAGACGCTTCCCCTGGCCGGGGTCCCGTTTGCGGTGAAGGAC
AATATCGACGTCGCGGACCTGGCCACGACAGCCGCCTGCCCAGGCTTCGCCTACACGGCCGGGCGCTCGGCGACCGTGGT
CGAGCAGCTGCTGGCCGCCGGGGCGGTGCTGGTCGGCAAGACCAATCTCGACCAGTTCGCAACGGGCCTCGTCGGGACGC
GCAGCCCCTACGGTGCGCCGCGCTGCGTTTTCAACCAGGCCTTCGTGAGCGGCGGCTCCAGCTCGGGCTCGGCCGTAGCC
GTGGCGGCCGGGCTGGTCGCCTTCGCACTGGGCACCGACACCGCCGGGTCCGGCCGGGTGCCGGCCGCCTTCAATCATCT
GGTCGGCCTGAAGCCCAGCAAGGGCCGATGGAGCACGCGCGGCCTCGTGCCCGCTTGCCGCTCGCTGGACTGCATCAGCG
TGTTCGCCGCCGATCTGGACGGGGCGGGACGGGTCGATGCGGTCCTGGCCGCCTTCGACCCCGAGGACCCCTACTCGCGC
CCCGCGCCCCAGCCGGAACCGGCCCTGACCCTGGCGGGGCTGCGGGTGGCGATTCCCCGGCCCGAGCAGCGGATCTTCTT
CGGCGACGGCGAGTCCGAGGCGCTGTACGCCTCTGCCGTCGCGCGCCTGCAGGCGGCCGGCGCAGTCGCCGTCGAGGTCG
ACATGGCCCCGCTGCTGGATGCGGCCAAGCTGCTCTACAGCGGCCCCTGGGTGGCCGAGCGCACCGCCGCCGTCGAGCCG
CTGCTGCGCGCCGCGCCCAGCGCCATCGAGCCGACCGTGCGGGCCATCGTCCAGGGCGGCCTGGCCGTGACCGGAGTCGA
GACCTTCCGAGGGCTCTATGCGCTGGAGGCCTATCGCCGGGCGGCCGAGGCCGTATGGGCCGCCGCCGACGCGCTGTTCC
TGCCCACGACGCCGACCATCTACACGGTCAAGGCGCTGAAGGCGGAACCCCTGGCCCTGAACGCCAATCTGGGCCTCTAC
ACCAATTTCGTGAACCTGCTGGACCTGTCGGCCCTGGCGGTTCCGGCAGGGTTCCGCGCCGACGGCACGGGCTTTGGGGT
CACCTTCATCGGCCCCGCTTTCGCCGACCGCGCCCTTCTGGACCTCGCCGCCGTCTATCTGGAGACCTTCCCCATGGCCG
AGACCCCGCCGCTGGACCTGACGCCCCACAAGCCCGGCGTGAAGCTGGCCGTGGTCGGGGCGCATCTGTCAGGCATGCCT
CTGCACTGGCAACTGACCTCGCGCGAAGCTCGGCTGGTCGCAGCGACGCGCACAGCTCCGGCTTACAAGCTCTACGCCAT
GGCCGAGAGCACCCCGCCCAAGCCAGCGCTCGTCCATGTCGGCGACGGCGGGGCGTCGATCCTGGTCGAAGTCTACGAAC
TGGACGTCGCGGCGTTCGGGGCCTTCACCGCTGAGGTCCCCGCCCCACTGGCGATCGGTACGGTGACCCTGGAGGACGGA
ACCAGCGTCAAGGGTTTCGTCGCCGAGCCCCGCGCCCTGAACGGCGCGAGCGACATCACCGTCCTGGGCGGCTGGCGGGC
CTATATCGCCTCGCTGCAGGGCTGA

Upstream 100 bases:

>100_bases
CGACGGCCGGTGTCGTCAGCGCCGTCTATGCCCAGCCGGGCCAGCCGATCGCCGCCGGCGCGCCGGTCATCGCTGTCACC
CCGGTTGCGGAGGCTGCGGC

Downstream 100 bases:

>100_bases
GTCCCCCAAGTGCGCCAGGCGTTTCTGTTGTCTTGAGGCGCTACTGGCCCAGACCCGCCAGCGCCTCGGGCCCGACCGCG
CCGGGCTTGTCCAGCGGGAA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNAPRLLVGEIAAGVNAGDSAESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVDARIAAGETLPLAGVPFAVKD
NIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGKTNLDQFATGLVGTRSPYGAPRCVFNQAFVSGGSSSGSAVA
VAAGLVAFALGTDTAGSGRVPAAFNHLVGLKPSKGRWSTRGLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSR
PAPQPEPALTLAGLRVAIPRPEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLDAAKLLYSGPWVAERTAAVEP
LLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRRAAEAVWAAADALFLPTTPTIYTVKALKAEPLALNANLGLY
TNFVNLLDLSALAVPAGFRADGTGFGVTFIGPAFADRALLDLAAVYLETFPMAETPPLDLTPHKPGVKLAVVGAHLSGMP
LHWQLTSREARLVAATRTAPAYKLYAMAESTPPKPALVHVGDGGASILVEVYELDVAAFGAFTAEVPAPLAIGTVTLEDG
TSVKGFVAEPRALNGASDITVLGGWRAYIASLQG

Sequences:

>Translated_594_residues
MNAPRLLVGEIAAGVNAGDSAESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVDARIAAGETLPLAGVPFAVKD
NIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGKTNLDQFATGLVGTRSPYGAPRCVFNQAFVSGGSSSGSAVA
VAAGLVAFALGTDTAGSGRVPAAFNHLVGLKPSKGRWSTRGLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSR
PAPQPEPALTLAGLRVAIPRPEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLDAAKLLYSGPWVAERTAAVEP
LLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRRAAEAVWAAADALFLPTTPTIYTVKALKAEPLALNANLGLY
TNFVNLLDLSALAVPAGFRADGTGFGVTFIGPAFADRALLDLAAVYLETFPMAETPPLDLTPHKPGVKLAVVGAHLSGMP
LHWQLTSREARLVAATRTAPAYKLYAMAESTPPKPALVHVGDGGASILVEVYELDVAAFGAFTAEVPAPLAIGTVTLEDG
TSVKGFVAEPRALNGASDITVLGGWRAYIASLQG
>Mature_594_residues
MNAPRLLVGEIAAGVNAGDSAESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVDARIAAGETLPLAGVPFAVKD
NIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGKTNLDQFATGLVGTRSPYGAPRCVFNQAFVSGGSSSGSAVA
VAAGLVAFALGTDTAGSGRVPAAFNHLVGLKPSKGRWSTRGLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSR
PAPQPEPALTLAGLRVAIPRPEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLDAAKLLYSGPWVAERTAAVEP
LLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRRAAEAVWAAADALFLPTTPTIYTVKALKAEPLALNANLGLY
TNFVNLLDLSALAVPAGFRADGTGFGVTFIGPAFADRALLDLAAVYLETFPMAETPPLDLTPHKPGVKLAVVGAHLSGMP
LHWQLTSREARLVAATRTAPAYKLYAMAESTPPKPALVHVGDGGASILVEVYELDVAAFGAFTAEVPAPLAIGTVTLEDG
TSVKGFVAEPRALNGASDITVLGGWRAYIASLQG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=470, Percent_Identity=27.6595744680851, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI195972892, Length=480, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=404, Percent_Identity=27.970297029703, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=194, Percent_Identity=31.4432989690722, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=136, Percent_Identity=34.5588235294118, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=575, Percent_Identity=45.3913043478261, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323950, Length=220, Percent_Identity=34.5454545454545, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648113, Length=203, Percent_Identity=37.9310344827586, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356731, Length=166, Percent_Identity=34.3373493975904, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652985, Length=217, Percent_Identity=30.4147465437788, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922090, Length=217, Percent_Identity=30.4147465437788, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652981, Length=217, Percent_Identity=30.4147465437788, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652983, Length=217, Percent_Identity=30.4147465437788, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 60982; Mature: 60982

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPRLLVGEIAAGVNAGDSAESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVD
CCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
ARIAAGETLPLAGVPFAVKDNIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGK
HEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCEEEEEC
TNLDQFATGLVGTRSPYGAPRCVFNQAFVSGGSSSGSAVAVAAGLVAFALGTDTAGSGRV
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCCCC
PAAFNHLVGLKPSKGRWSTRGLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSR
CHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
PAPQPEPALTLAGLRVAIPRPEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLD
CCCCCCCCEEEECEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHH
AAKLLYSGPWVAERTAAVEPLLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
AAEAVWAAADALFLPTTPTIYTVKALKAEPLALNANLGLYTNFVNLLDLSALAVPAGFRA
HHHHHHHHHCEEEECCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
DGTGFGVTFIGPAFADRALLDLAAVYLETFPMAETPPLDLTPHKPGVKLAVVGAHLSGMP
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
LHWQLTSREARLVAATRTAPAYKLYAMAESTPPKPALVHVGDGGASILVEVYELDVAAFG
EEEEECCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEEEHHHHHHHC
AFTAEVPAPLAIGTVTLEDGTSVKGFVAEPRALNGASDITVLGGWRAYIASLQG
CEECCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECHHHHHHHCCC
>Mature Secondary Structure
MNAPRLLVGEIAAGVNAGDSAESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVD
CCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
ARIAAGETLPLAGVPFAVKDNIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGK
HEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCEEEEEC
TNLDQFATGLVGTRSPYGAPRCVFNQAFVSGGSSSGSAVAVAAGLVAFALGTDTAGSGRV
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCCCC
PAAFNHLVGLKPSKGRWSTRGLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSR
CHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
PAPQPEPALTLAGLRVAIPRPEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLD
CCCCCCCCEEEECEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHH
AAKLLYSGPWVAERTAAVEPLLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
AAEAVWAAADALFLPTTPTIYTVKALKAEPLALNANLGLYTNFVNLLDLSALAVPAGFRA
HHHHHHHHHCEEEECCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
DGTGFGVTFIGPAFADRALLDLAAVYLETFPMAETPPLDLTPHKPGVKLAVVGAHLSGMP
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
LHWQLTSREARLVAATRTAPAYKLYAMAESTPPKPALVHVGDGGASILVEVYELDVAAFG
EEEEECCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEEEHHHHHHHC
AFTAEVPAPLAIGTVTLEDGTSVKGFVAEPRALNGASDITVLGGWRAYIASLQG
CEECCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA