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The map label for this gene is purR [H]

Identifier: 16125997

GI number: 16125997

Start: 1929517

End: 1930563

Strand: Direct

Name: purR [H]

Synonym: CC_1753

Alternate gene names: 16125997

Gene position: 1929517-1930563 (Clockwise)

Preceding gene: 16125995

Following gene: 16125998

Centisome position: 48.03

GC content: 69.34

Gene sequence:

>1047_bases
TTGACCACCAAAAGACCTACGATCGACGATGTCGCCCGCCTGTCGGGTGTGGCGCGCGTCACGGTCTCGCGGGTGCTGAA
CGGTGGTCCGAACGTGCGCGACGAGGTCCGCGCGCGGGTCATGCAGGCGGTCGAGACGCTGGCGTACAAGGTCAATCCGC
AGGCGCGCAGCCTCGCGGGCGGGGCCAGCCGGCTGTTGGCCTTCGTCTTCGCGTCAGATCCCGAGAACGAGCCGAACTCG
TACTACGAATCCGCGCTGGAGCTGGGTGCGCTGCGCGCCTGCCTGGCGCTGGGCTACCAAGTGCTGGTCCAGAACGTTCC
GCAACAGACGCCCGATCACCAGAAGCGGATCCTGGACCTGATCACCACCCAACGCTGCGAGGGCCTGATCCTGTCGCCGC
CCTACAGCGACGACGTCGCGCTGATCGAGGCGGCGCTGGCGCAGGGCTGCAAGGTCGTGACCGTCTCGCCTGGCGGTCCG
GGACGCGGGAAGGCCGACGGGGTCGGCGTCGACGACGAGGCCGGCGGCTATGACATGACCCGCGAGCTCCTGCGTCTGGG
TCACCGACGATTCGGCTTCATCGCCGGCCTGGAAGGGCACCTTTCGGCCGACCGACGTCTGCAGGGCTTCCGCAGAGCGC
TCGGCGAGCAGGGGCTGGGGGAGGGCGACTGTGTCGTGCTCCGGGGGGACTTCACCTTCCGGTCGGGTACCCGCCTGGCG
CCGGAGCTTCTGGATCATCCGCTCAAGCCGACCGCGCTGGTCTGCGCCAACGACGACATGGCCGCCGGCGTCCTGTCCGC
CGCCCATGCGCGGGGGCTCAGTATCCCGCAGGACCTGTCGATCACAGGTTTCGACGATGCGCCTGTGGCCGGCATCGTCT
GGCCGCCGCTCACCACGGTGCACCAGCCGGTCAAGGTCCTGGGCCAGCGCGCCGTCGAGCTGCTGGTCGCGCGCCTGACG
GGCGTCAACGTCTCCGACGCGGCGATTTTCGACGAGCTCGAGCATGCCGTCGTGACACGGCAAACCACCACCGCGCCCCA
CCTCTAG

Upstream 100 bases:

>100_bases
AGGGCGGGTCTCTGTCGGTCCGTGCGCGTCATGACGTTGAGCCGCTTGTGTTGCTCTGCTTTCCTGACCATCTTCCCGGC
GCTCGAAGGGGGAGCTCCAT

Downstream 100 bases:

>100_bases
ACCGCCTGAGCTTGACAACGTTGTCGCCTTGAGCGCAATTGGAAGCGCTTCCATAGGGGCGCATTCACGGCTACTGGCCT
GACAACAAGAACGATATCTC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTTKRPTIDDVARLSGVARVTVSRVLNGGPNVRDEVRARVMQAVETLAYKVNPQARSLAGGASRLLAFVFASDPENEPNS
YYESALELGALRACLALGYQVLVQNVPQQTPDHQKRILDLITTQRCEGLILSPPYSDDVALIEAALAQGCKVVTVSPGGP
GRGKADGVGVDDEAGGYDMTRELLRLGHRRFGFIAGLEGHLSADRRLQGFRRALGEQGLGEGDCVVLRGDFTFRSGTRLA
PELLDHPLKPTALVCANDDMAAGVLSAAHARGLSIPQDLSITGFDDAPVAGIVWPPLTTVHQPVKVLGQRAVELLVARLT
GVNVSDAAIFDELEHAVVTRQTTTAPHL

Sequences:

>Translated_348_residues
MTTKRPTIDDVARLSGVARVTVSRVLNGGPNVRDEVRARVMQAVETLAYKVNPQARSLAGGASRLLAFVFASDPENEPNS
YYESALELGALRACLALGYQVLVQNVPQQTPDHQKRILDLITTQRCEGLILSPPYSDDVALIEAALAQGCKVVTVSPGGP
GRGKADGVGVDDEAGGYDMTRELLRLGHRRFGFIAGLEGHLSADRRLQGFRRALGEQGLGEGDCVVLRGDFTFRSGTRLA
PELLDHPLKPTALVCANDDMAAGVLSAAHARGLSIPQDLSITGFDDAPVAGIVWPPLTTVHQPVKVLGQRAVELLVARLT
GVNVSDAAIFDELEHAVVTRQTTTAPHL
>Mature_347_residues
TTKRPTIDDVARLSGVARVTVSRVLNGGPNVRDEVRARVMQAVETLAYKVNPQARSLAGGASRLLAFVFASDPENEPNSY
YESALELGALRACLALGYQVLVQNVPQQTPDHQKRILDLITTQRCEGLILSPPYSDDVALIEAALAQGCKVVTVSPGGPG
RGKADGVGVDDEAGGYDMTRELLRLGHRRFGFIAGLEGHLSADRRLQGFRRALGEQGLGEGDCVVLRGDFTFRSGTRLAP
ELLDHPLKPTALVCANDDMAAGVLSAAHARGLSIPQDLSITGFDDAPVAGIVWPPLTTVHQPVKVLGQRAVELLVARLTG
VNVSDAAIFDELEHAVVTRQTTTAPHL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=33.015873015873, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787948, Length=320, Percent_Identity=32.1875, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=31.858407079646, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789068, Length=342, Percent_Identity=28.6549707602339, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789202, Length=306, Percent_Identity=31.6993464052288, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=32.6589595375723, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1786540, Length=350, Percent_Identity=30.5714285714286, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=32.9153605015674, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=27.0186335403727, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=25.9146341463415, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=23.8532110091743, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787906, Length=328, Percent_Identity=25.3048780487805, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=24.3670886075949, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37118; Mature: 36987

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKRPTIDDVARLSGVARVTVSRVLNGGPNVRDEVRARVMQAVETLAYKVNPQARSLAG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GASRLLAFVFASDPENEPNSYYESALELGALRACLALGYQVLVQNVPQQTPDHQKRILDL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ITTQRCEGLILSPPYSDDVALIEAALAQGCKVVTVSPGGPGRGKADGVGVDDEAGGYDMT
HHHHHCCCEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RELLRLGHRRFGFIAGLEGHLSADRRLQGFRRALGEQGLGEGDCVVLRGDFTFRSGTRLA
HHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEECCCCCCC
PELLDHPLKPTALVCANDDMAAGVLSAAHARGLSIPQDLSITGFDDAPVAGIVWPPLTTV
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCCHHHH
HQPVKVLGQRAVELLVARLTGVNVSDAAIFDELEHAVVTRQTTTAPHL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTKRPTIDDVARLSGVARVTVSRVLNGGPNVRDEVRARVMQAVETLAYKVNPQARSLAG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GASRLLAFVFASDPENEPNSYYESALELGALRACLALGYQVLVQNVPQQTPDHQKRILDL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ITTQRCEGLILSPPYSDDVALIEAALAQGCKVVTVSPGGPGRGKADGVGVDDEAGGYDMT
HHHHHCCCEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RELLRLGHRRFGFIAGLEGHLSADRRLQGFRRALGEQGLGEGDCVVLRGDFTFRSGTRLA
HHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEECCCCCCC
PELLDHPLKPTALVCANDDMAAGVLSAAHARGLSIPQDLSITGFDDAPVAGIVWPPLTTV
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCCHHHH
HQPVKVLGQRAVELLVARLTGVNVSDAAIFDELEHAVVTRQTTTAPHL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA