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The map label for this gene is btuB [H]

Identifier: 16125994

GI number: 16125994

Start: 1925322

End: 1927274

Strand: Direct

Name: btuB [H]

Synonym: CC_1750

Alternate gene names: 16125994

Gene position: 1925322-1927274 (Clockwise)

Preceding gene: 16125993

Following gene: 16125995

Centisome position: 47.93

GC content: 67.59

Gene sequence:

>1953_bases
ATGATCCGAGCCCTCTTGAGCTCCACCGCCCTGGCCACCGCGCTGCTGGCTGCTCCGGCCCTCGCCGATGACCAGAAGGC
CCCTGAAGCCGACGTCGCCGGCGCCACCGGCGCCAACGGCGTCGCCGAACTGGTGGTCACCGCCACCCGCACCGTGCAAC
CGATCGAGAAGGTCGGCGCCGCGGTGACCGTCCTGACCCAGCCCGCGATCGAGGCCAGCCAGGCCATCTCGGTCACCGAA
CTGCTGGCCCAGACGCCCAGCGTGACCTTCGCCCGCAACGGCGGCGTCGGCACGGCGACCAGCCTTTATATCCGCGGCGC
CGAGAGCCATCACACCGTCGTGCTGATCGACGGCGTCAAGCTGAACGATCCGTCGTCCATCCAGGGCGGCTTCAACAGCG
GCAACCTGCTGGTCGGCGACATCTCGCGCATCGAAGTGCTGCGCGGCGCGCAATCCACCCTCTGGGGCAGCCAGGCGGTC
GGCGGCGTCGTCAACATTGTCACCGCCGAACCGACCACGCCGTTCACCAGCTCGCTGTCGGCCGAGGCCGGCGCGCGCAA
AACCGGCTATCTGCGCGGCGCCGTCGGCGGCGCTTCGGACAAGGTCACCTGGCGTGTCGCGGGCGGCTATTACACCACCG
ACGGTTTCTCCGCCCACAAGACGGGCAAGGAGGCGGACGGCTATCAGAACGCGGGCGCCTCGGGCCGCCTGCGGGTGAAA
CTGACCGATGCGGTCTCGGTCGAGACCCGGGCGGTCTATTCGCGCGGCAAGAACGACTTCGACGGCTTCAACACCGACAG
CCCCGAGTTTGGCCGCACCAAGGAGCTGGTGATCTATCAAGGCCTGAACGCCGCCTTGCTGGACGGTCGTTGGAACAACC
GCGTCGGCTTTGCCTATACCGACACCGACCGCGAGAACTACAATCCCGCCCGCGCCGCCGTGCCGATGACGTTCGATGCG
GCCGGCAAGAACAAGCGCTGGGAATATCAGGGCGTGTTCGCGATCACCGAGACCTGGACCGCGACCTTCGGCGCCGAGAG
CGAACGCTCGCGCATGCGCACCCGCTCGCCGTCGGTCGCCAATCCGAACGTGGCGTTCCGCACCGGCAAGACCAGCGCCG
ACAGCCTGTATGGCCAACTGCAGGCCGAGATCGCCCCCGGCCTGACGGTGACCGGCGGTCTGCGCTACGAAGACCACGAC
GCCTACGGCGCCCACACGCTGGGGCAAGTCGGCGCGGCCTGGGCCCTGAATGACAACGCCACGGTTCTTCGTGCCGGCTA
CAGCCAAGGCTTCCGCGCGCCGGGCCTCTATGAGCTGTTCAGCGAGTACGGCAATCCCGCGCTGTCTCCTGAAGAGTTCG
ACAGCTGGGAAGCCGGCGTCGAGCAGCGCTTTCTGGAGGGCAAGGTCCGCGCCTCGGCGACGGTCTTCCACCGTGAGGCC
GACAACGAGATCCGCTTCTTCTCGTGCTCGTTCGGCGCGACTGACACGATATGCGCGCCGGGCGGGGTCTTCCGGTTCGG
CTACTACCGCAATCTGCAGAAGACCAAGGCCCAGGGGCTGGAGCTGATCGCCGAGGCGCGCCCGGTCGAGGGCCTGACGA
TCACCGGCAACTACACCTATACCGAGGCCGAACTGGCGGCGGGCGCGAACACCGGCAAGCAGCTGTCGCGTCGCCCGAAG
GACCTCGCCAACCTGTCGGCGACCTATCGCTGGCCGGTGGGGCTCTCGACCACGGTGGCGGTGCGCTATGTGGGCAAGAC
CTACAATAACGAGGCCAACACCGTGGCGGTGTCGGACTATGCGACGGTGGATCTGCGGGCGTCCTATCCGATCAGCGAGA
CCCTGGAGGTCTATGGTCGGGTCGAGAACGCCTTGGACAAGGACTATCAGACGATCCTGAACTACGGCACGCCGGGCCGT
GGCGCGTTCCTGGGTCTGCGGGCGCGCTTCTAG

Upstream 100 bases:

>100_bases
GAGAAACGACAGTCAACAGGCCGCGCGACGGCCTGAGCGTCCGCGTCTTCGCGGGCGGTCGGGAGGTCGCGTGGGTCGTT
CATAACGGGAAGACTGTATT

Downstream 100 bases:

>100_bases
GGATGGGAGGGGCGGGGGGCTGTGTCCCTCGCCCCCGGCCACCTTGTCTTGGAGGAAAGCAGCGCATGGCCGCCTTGATG
ATCCAGGGCTGCGGCTCGGA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MIRALLSSTALATALLAAPALADDQKAPEADVAGATGANGVAELVVTATRTVQPIEKVGAAVTVLTQPAIEASQAISVTE
LLAQTPSVTFARNGGVGTATSLYIRGAESHHTVVLIDGVKLNDPSSIQGGFNSGNLLVGDISRIEVLRGAQSTLWGSQAV
GGVVNIVTAEPTTPFTSSLSAEAGARKTGYLRGAVGGASDKVTWRVAGGYYTTDGFSAHKTGKEADGYQNAGASGRLRVK
LTDAVSVETRAVYSRGKNDFDGFNTDSPEFGRTKELVIYQGLNAALLDGRWNNRVGFAYTDTDRENYNPARAAVPMTFDA
AGKNKRWEYQGVFAITETWTATFGAESERSRMRTRSPSVANPNVAFRTGKTSADSLYGQLQAEIAPGLTVTGGLRYEDHD
AYGAHTLGQVGAAWALNDNATVLRAGYSQGFRAPGLYELFSEYGNPALSPEEFDSWEAGVEQRFLEGKVRASATVFHREA
DNEIRFFSCSFGATDTICAPGGVFRFGYYRNLQKTKAQGLELIAEARPVEGLTITGNYTYTEAELAAGANTGKQLSRRPK
DLANLSATYRWPVGLSTTVAVRYVGKTYNNEANTVAVSDYATVDLRASYPISETLEVYGRVENALDKDYQTILNYGTPGR
GAFLGLRARF

Sequences:

>Translated_650_residues
MIRALLSSTALATALLAAPALADDQKAPEADVAGATGANGVAELVVTATRTVQPIEKVGAAVTVLTQPAIEASQAISVTE
LLAQTPSVTFARNGGVGTATSLYIRGAESHHTVVLIDGVKLNDPSSIQGGFNSGNLLVGDISRIEVLRGAQSTLWGSQAV
GGVVNIVTAEPTTPFTSSLSAEAGARKTGYLRGAVGGASDKVTWRVAGGYYTTDGFSAHKTGKEADGYQNAGASGRLRVK
LTDAVSVETRAVYSRGKNDFDGFNTDSPEFGRTKELVIYQGLNAALLDGRWNNRVGFAYTDTDRENYNPARAAVPMTFDA
AGKNKRWEYQGVFAITETWTATFGAESERSRMRTRSPSVANPNVAFRTGKTSADSLYGQLQAEIAPGLTVTGGLRYEDHD
AYGAHTLGQVGAAWALNDNATVLRAGYSQGFRAPGLYELFSEYGNPALSPEEFDSWEAGVEQRFLEGKVRASATVFHREA
DNEIRFFSCSFGATDTICAPGGVFRFGYYRNLQKTKAQGLELIAEARPVEGLTITGNYTYTEAELAAGANTGKQLSRRPK
DLANLSATYRWPVGLSTTVAVRYVGKTYNNEANTVAVSDYATVDLRASYPISETLEVYGRVENALDKDYQTILNYGTPGR
GAFLGLRARF
>Mature_650_residues
MIRALLSSTALATALLAAPALADDQKAPEADVAGATGANGVAELVVTATRTVQPIEKVGAAVTVLTQPAIEASQAISVTE
LLAQTPSVTFARNGGVGTATSLYIRGAESHHTVVLIDGVKLNDPSSIQGGFNSGNLLVGDISRIEVLRGAQSTLWGSQAV
GGVVNIVTAEPTTPFTSSLSAEAGARKTGYLRGAVGGASDKVTWRVAGGYYTTDGFSAHKTGKEADGYQNAGASGRLRVK
LTDAVSVETRAVYSRGKNDFDGFNTDSPEFGRTKELVIYQGLNAALLDGRWNNRVGFAYTDTDRENYNPARAAVPMTFDA
AGKNKRWEYQGVFAITETWTATFGAESERSRMRTRSPSVANPNVAFRTGKTSADSLYGQLQAEIAPGLTVTGGLRYEDHD
AYGAHTLGQVGAAWALNDNATVLRAGYSQGFRAPGLYELFSEYGNPALSPEEFDSWEAGVEQRFLEGKVRASATVFHREA
DNEIRFFSCSFGATDTICAPGGVFRFGYYRNLQKTKAQGLELIAEARPVEGLTITGNYTYTEAELAAGANTGKQLSRRPK
DLANLSATYRWPVGLSTTVAVRYVGKTYNNEANTVAVSDYATVDLRASYPISETLEVYGRVENALDKDYQTILNYGTPGR
GAFLGLRARF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=621, Percent_Identity=27.0531400966184, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788478, Length=670, Percent_Identity=25.0746268656716, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786798, Length=688, Percent_Identity=23.546511627907, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787723, Length=691, Percent_Identity=23.2995658465991, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786344, Length=173, Percent_Identity=32.3699421965318, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 69353; Mature: 69353

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRALLSSTALATALLAAPALADDQKAPEADVAGATGANGVAELVVTATRTVQPIEKVGA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHHHHHHHCCC
AVTVLTQPAIEASQAISVTELLAQTPSVTFARNGGVGTATSLYIRGAESHHTVVLIDGVK
EEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEECEE
LNDPSSIQGGFNSGNLLVGDISRIEVLRGAQSTLWGSQAVGGVVNIVTAEPTTPFTSSLS
ECCCCCCCCCCCCCCEEEECHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
AEAGARKTGYLRGAVGGASDKVTWRVAGGYYTTDGFSAHKTGKEADGYQNAGASGRLRVK
CCCCCCCCCEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
LTDAVSVETRAVYSRGKNDFDGFNTDSPEFGRTKELVIYQGLNAALLDGRWNNRVGFAYT
EECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEE
DTDRENYNPARAAVPMTFDAAGKNKRWEYQGVFAITETWTATFGAESERSRMRTRSPSVA
CCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCCCC
NPNVAFRTGKTSADSLYGQLQAEIAPGLTVTGGLRYEDHDAYGAHTLGQVGAAWALNDNA
CCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCHHHHHCCCEEEECCCC
TVLRAGYSQGFRAPGLYELFSEYGNPALSPEEFDSWEAGVEQRFLEGKVRASATVFHREA
EEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCHHHCEEEEEEECC
DNEIRFFSCSFGATDTICAPGGVFRFGYYRNLQKTKAQGLELIAEARPVEGLTITGNYTY
CCCEEEEEECCCCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEE
TEAELAAGANTGKQLSRRPKDLANLSATYRWPVGLSTTVAVRYVGKTYNNEANTVAVSDY
EHHHHHCCCCCCHHHHCCCHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCEEEEECE
ATVDLRASYPISETLEVYGRVENALDKDYQTILNYGTPGRGAFLGLRARF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MIRALLSSTALATALLAAPALADDQKAPEADVAGATGANGVAELVVTATRTVQPIEKVGA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHHHHHHHCCC
AVTVLTQPAIEASQAISVTELLAQTPSVTFARNGGVGTATSLYIRGAESHHTVVLIDGVK
EEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEECEE
LNDPSSIQGGFNSGNLLVGDISRIEVLRGAQSTLWGSQAVGGVVNIVTAEPTTPFTSSLS
ECCCCCCCCCCCCCCEEEECHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
AEAGARKTGYLRGAVGGASDKVTWRVAGGYYTTDGFSAHKTGKEADGYQNAGASGRLRVK
CCCCCCCCCEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
LTDAVSVETRAVYSRGKNDFDGFNTDSPEFGRTKELVIYQGLNAALLDGRWNNRVGFAYT
EECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEE
DTDRENYNPARAAVPMTFDAAGKNKRWEYQGVFAITETWTATFGAESERSRMRTRSPSVA
CCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCCCC
NPNVAFRTGKTSADSLYGQLQAEIAPGLTVTGGLRYEDHDAYGAHTLGQVGAAWALNDNA
CCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCHHHHHCCCEEEECCCC
TVLRAGYSQGFRAPGLYELFSEYGNPALSPEEFDSWEAGVEQRFLEGKVRASATVFHREA
EEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCHHHCEEEEEEECC
DNEIRFFSCSFGATDTICAPGGVFRFGYYRNLQKTKAQGLELIAEARPVEGLTITGNYTY
CCCEEEEEECCCCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEE
TEAELAAGANTGKQLSRRPKDLANLSATYRWPVGLSTTVAVRYVGKTYNNEANTVAVSDY
EHHHHHCCCCCCHHHHCCCHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCEEEEECE
ATVDLRASYPISETLEVYGRVENALDKDYQTILNYGTPGRGAFLGLRARF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA