Click here to switch to the map view.

The map label for this gene is rlmE

Identifier: 16125862

GI number: 16125862

Start: 1788163

End: 1788843

Strand: Direct

Name: rlmE

Synonym: CC_1615

Alternate gene names: 16125862

Gene position: 1788163-1788843 (Clockwise)

Preceding gene: 16125861

Following gene: 16125863

Centisome position: 44.52

GC content: 67.11

Gene sequence:

>681_bases
ATGGTCAAGCCGCCGGCCGGCGGCAATGAAGGCGGACGCGGCAAGCCCGCGCGCCTGAAGACCGCCTATGGCCGCACCCC
CAGCCAGCAGGCCTGGCTGGAGCGACAGATCAACGATCCCTTCTCGGCCAAGGCCCGGGCGCTGGGCTACCGCAGCCGGG
CGGCGTTCAAGATCAGCGAGATCGACGACAAGTTCCATTTCTTCAGCAAGGGCGCGAAGGTCATCGACCTGGGCTGCGCG
CCGGGCGGCTGGCTGCAGATCGCCGTCGAACGCGGCGTGACGACCCTGGCGGGGATCGACCTCCTGCCGGTCGATCCCGT
GGCGCCCGCCCATCTTCTGGAGATGGACTTCACCGCCGACGGCGCGCCCGAGAAGCTTCTGGAGCTTTTGGGCGGCGAAC
CTGATCTTGTTATGTCCGACATGGCGCCCAACACCGTGGGCCACCGCGAGACCGACCACCTGCGGATCGTCGGCCTGATC
GAGATCGCCGCCGAGTTCGCGATCGATGTCCTCAAGCCCGGCGGCGCGTTCGTCGCCAAGGCGTTCCAGGGCGGCGAGAC
GGCCGAGATCATCGGCAAGCTCAAGCGCCACTTCGACAAGGTCCAGCACTTCAAGCCCAAGGCCAGCCGGCAGGACAGCT
CCGAGGTCTTCCTGGTTGCGACAGGATTCAAGGGACGGTGA

Upstream 100 bases:

>100_bases
TGATGTCCGATCAGCAGCGACGCCCTCGGCGGCGTCGTCGCGGCGGCGCCTCCAAGAAGTCTGAAGCCGCATGAGTGACG
AAGAGCCGCCCCGCAAGCGC

Downstream 100 bases:

>100_bases
GGCGAGGGGAGCGCGTGATCGGCGGGCTGATCGCGCGGCCCTTCCGCAACCTGGCCTTCGCCGTGGCGTTCGTTCTCGCC
GTCGCGGGCATGGCGGTTCT

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MVKPPAGGNEGGRGKPARLKTAYGRTPSQQAWLERQINDPFSAKARALGYRSRAAFKISEIDDKFHFFSKGAKVIDLGCA
PGGWLQIAVERGVTTLAGIDLLPVDPVAPAHLLEMDFTADGAPEKLLELLGGEPDLVMSDMAPNTVGHRETDHLRIVGLI
EIAAEFAIDVLKPGGAFVAKAFQGGETAEIIGKLKRHFDKVQHFKPKASRQDSSEVFLVATGFKGR

Sequences:

>Translated_226_residues
MVKPPAGGNEGGRGKPARLKTAYGRTPSQQAWLERQINDPFSAKARALGYRSRAAFKISEIDDKFHFFSKGAKVIDLGCA
PGGWLQIAVERGVTTLAGIDLLPVDPVAPAHLLEMDFTADGAPEKLLELLGGEPDLVMSDMAPNTVGHRETDHLRIVGLI
EIAAEFAIDVLKPGGAFVAKAFQGGETAEIIGKLKRHFDKVQHFKPKASRQDSSEVFLVATGFKGR
>Mature_226_residues
MVKPPAGGNEGGRGKPARLKTAYGRTPSQQAWLERQINDPFSAKARALGYRSRAAFKISEIDDKFHFFSKGAKVIDLGCA
PGGWLQIAVERGVTTLAGIDLLPVDPVAPAHLLEMDFTADGAPEKLLELLGGEPDLVMSDMAPNTVGHRETDHLRIVGLI
EIAAEFAIDVLKPGGAFVAKAFQGGETAEIIGKLKRHFDKVQHFKPKASRQDSSEVFLVATGFKGR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=36.2790697674419, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=36.1702127659575, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=32.1052631578947, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=32.1052631578947, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=32.1052631578947, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=41.5458937198068, Blast_Score=149, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=201, Percent_Identity=38.8059701492537, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=33.6787564766839, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=188, Percent_Identity=34.5744680851064, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=34.5744680851064, Blast_Score=106, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=31.7948717948718, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=33.6683417085427, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321302, Length=287, Percent_Identity=27.8745644599303, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=33.5078534031414, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356387, Length=230, Percent_Identity=39.1304347826087, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=35.1063829787234, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=32.1428571428571, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=29.7979797979798, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_CAUCR (Q9A7V4)

Other databases:

- EMBL:   AE005673
- PIR:   F87449
- RefSeq:   NP_420426.1
- ProteinModelPortal:   Q9A7V4
- SMR:   Q9A7V4
- GeneID:   940586
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_1615
- NMPDR:   fig|190650.1.peg.1606
- TIGR:   CC_1615
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK890513
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24356; Mature: 24356

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 82-82 BINDING 84-84 BINDING 100-100 BINDING 116-116 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKPPAGGNEGGRGKPARLKTAYGRTPSQQAWLERQINDPFSAKARALGYRSRAAFKISE
CCCCCCCCCCCCCCCCCEEEHHCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEHH
IDDKFHFFSKGAKVIDLGCAPGGWLQIAVERGVTTLAGIDLLPVDPVAPAHLLEMDFTAD
HCHHHHHHHCCCEEEEECCCCCCEEEEEEHHCCHHHHCCEEECCCCCCCHHEEEECCCCC
GAPEKLLELLGGEPDLVMSDMAPNTVGHRETDHLRIVGLIEIAAEFAIDVLKPGGAFVAK
CCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHH
AFQGGETAEIIGKLKRHFDKVQHFKPKASRQDSSEVFLVATGFKGR
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MVKPPAGGNEGGRGKPARLKTAYGRTPSQQAWLERQINDPFSAKARALGYRSRAAFKISE
CCCCCCCCCCCCCCCCCEEEHHCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEHH
IDDKFHFFSKGAKVIDLGCAPGGWLQIAVERGVTTLAGIDLLPVDPVAPAHLLEMDFTAD
HCHHHHHHHCCCEEEEECCCCCCEEEEEEHHCCHHHHCCEEECCCCCCCHHEEEECCCCC
GAPEKLLELLGGEPDLVMSDMAPNTVGHRETDHLRIVGLIEIAAEFAIDVLKPGGAFVAK
CCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHH
AFQGGETAEIIGKLKRHFDKVQHFKPKASRQDSSEVFLVATGFKGR
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647