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The map label for this gene is pdxJ

Identifier: 16125804

GI number: 16125804

Start: 1721592

End: 1722356

Strand: Direct

Name: pdxJ

Synonym: CC_1557

Alternate gene names: 16125804

Gene position: 1721592-1722356 (Clockwise)

Preceding gene: 16125802

Following gene: 16125805

Centisome position: 42.86

GC content: 69.54

Gene sequence:

>765_bases
ATGAGCTTGCGGCTGGGCGTCAATATCGATCACGTGGCCACGATCCGGAACGCGCGCGGCGCTTCGTATCCCGAGCCCGT
CCGCGCGGCCGAGCTGGCCTTGATCGCCGGCGCCGACGGCATCACCGCCCACCTGCGCGAGGATCGCCGACACATCAGTG
ACGCCGACATCGCGGTGCTGACCGACCTCTGCCACAAGCGCGGCAAGCCGTTGAACTTCGAGATGGCGGTCACCGACGAG
ATGGTCGGCATCGCCCTGAACGCGCGTCCGCACGCCGCGTGCCTGGTGCCCGAGCGCCGCGAGGAGGTCACCACCGAGGG
CGGCCTCGACGTCATCAAGGGCCAGAAGCGTATCGCCGACGCCACCGCGCGGCTGCGCACCGTGGGGGCGCGGGTCTCGC
TGTTCATCGAGCCCGATCCCGATCAGATCCGCGCCTGCGTCACGGCGGGGGCTCAAGTGGTCGAGCTGCACACCGGCGCC
TATTGTGACGCGGCGCGGGCGGGTGAGACGGCGCGGGCCGAGGCGATCCTGAAGCGGCTCAAGGCCGGCGCCGCCTTGGC
CCATGAGCTTGGCCTTGAGGTCCATGCCGGGCATGGCATCGACTACGCCACGGTGAAGCCGGTCGCCGCCATCCCGCAGA
TCGCCGAGCTCAATATCGGCCACTTCCTGATCGGTGAGGCGATCTTCGTGGGCCTGCCTGAAGCGATCCACCGCATGCGG
GCCCTGATGGAGGCCGCGCGCGTCGAACTTGAGGTCCTGGCGTGA

Upstream 100 bases:

>100_bases
CCTGTTGCAAAGCCTCACCTGCCCAAGTGAGGTGCGGCGCCCTAGGATTCGGGCGGCGGGGGGAGTAACCCTGCCGTCGA
AGGCAGGAGGACCTGAGCGC

Downstream 100 bases:

>100_bases
TCATCGGCATCGGCTCGGACCTCTGCGACATCCGGCGGATCGAGAAGTCGCTGGAGCGCTTCGGCGACCGCTTTACCCAC
AAGGTCTTCACCGAGACCGA

Product: pyridoxal phosphate biosynthetic protein PdxJ

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSLRLGVNIDHVATIRNARGASYPEPVRAAELALIAGADGITAHLREDRRHISDADIAVLTDLCHKRGKPLNFEMAVTDE
MVGIALNARPHAACLVPERREEVTTEGGLDVIKGQKRIADATARLRTVGARVSLFIEPDPDQIRACVTAGAQVVELHTGA
YCDAARAGETARAEAILKRLKAGAALAHELGLEVHAGHGIDYATVKPVAAIPQIAELNIGHFLIGEAIFVGLPEAIHRMR
ALMEAARVELEVLA

Sequences:

>Translated_254_residues
MSLRLGVNIDHVATIRNARGASYPEPVRAAELALIAGADGITAHLREDRRHISDADIAVLTDLCHKRGKPLNFEMAVTDE
MVGIALNARPHAACLVPERREEVTTEGGLDVIKGQKRIADATARLRTVGARVSLFIEPDPDQIRACVTAGAQVVELHTGA
YCDAARAGETARAEAILKRLKAGAALAHELGLEVHAGHGIDYATVKPVAAIPQIAELNIGHFLIGEAIFVGLPEAIHRMR
ALMEAARVELEVLA
>Mature_253_residues
SLRLGVNIDHVATIRNARGASYPEPVRAAELALIAGADGITAHLREDRRHISDADIAVLTDLCHKRGKPLNFEMAVTDEM
VGIALNARPHAACLVPERREEVTTEGGLDVIKGQKRIADATARLRTVGARVSLFIEPDPDQIRACVTAGAQVVELHTGAY
CDAARAGETARAEAILKRLKAGAALAHELGLEVHAGHGIDYATVKPVAAIPQIAELNIGHFLIGEAIFVGLPEAIHRMRA
LMEAARVELEVLA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=245, Percent_Identity=53.469387755102, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_CAUCN (B8H623)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516999.1
- ProteinModelPortal:   B8H623
- SMR:   B8H623
- GeneID:   7331604
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01626
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27132; Mature: 27000

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 74-74 ACT_SITE 198-198 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 104-104 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLGVNIDHVATIRNARGASYPEPVRAAELALIAGADGITAHLREDRRHISDADIAVL
CEEEECCCCHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHCCCCHHHHH
TDLCHKRGKPLNFEMAVTDEMVGIALNARPHAACLVPERREEVTTEGGLDVIKGQKRIAD
HHHHHHCCCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHHHCCCCCHHHHHCHHHHHH
ATARLRTVGARVSLFIEPDPDQIRACVTAGAQVVELHTGAYCDAARAGETARAEAILKRL
HHHHHHHHCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHH
KAGAALAHELGLEVHAGHGIDYATVKPVAAIPQIAELNIGHFLIGEAIFVGLPEAIHRMR
HHHHHHHHHHCCEEECCCCCCHHHCCCHHHCCHHHHCCCCHHHHHHHHHHCCHHHHHHHH
ALMEAARVELEVLA
HHHHHHHHEEEEEC
>Mature Secondary Structure 
SLRLGVNIDHVATIRNARGASYPEPVRAAELALIAGADGITAHLREDRRHISDADIAVL
EEEECCCCHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHCCCCHHHHH
TDLCHKRGKPLNFEMAVTDEMVGIALNARPHAACLVPERREEVTTEGGLDVIKGQKRIAD
HHHHHHCCCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHHHCCCCCHHHHHCHHHHHH
ATARLRTVGARVSLFIEPDPDQIRACVTAGAQVVELHTGAYCDAARAGETARAEAILKRL
HHHHHHHHCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHH
KAGAALAHELGLEVHAGHGIDYATVKPVAAIPQIAELNIGHFLIGEAIFVGLPEAIHRMR
HHHHHHHHHHCCEEECCCCCCHHHCCCHHHCCHHHHCCCCHHHHHHHHHHCCHHHHHHHH
ALMEAARVELEVLA
HHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA