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The map label for this gene is metG

Identifier: 16125727

GI number: 16125727

Start: 1633459

End: 1635168

Strand: Direct

Name: metG

Synonym: CC_1480

Alternate gene names: 16125727

Gene position: 1633459-1635168 (Clockwise)

Preceding gene: 16125725

Following gene: 16125728

Centisome position: 40.66

GC content: 66.37

Gene sequence:

>1710_bases
ATGGCTCGCATCCTGATTACCTCCGCCCTGCCGTACATCAACGGCATCAAGCACCTGGGGAACCTCGCGGGCTCCATGCT
GCCGGCGGACGTCTATGCGCGCTTCAAGCGGGCCCAGGGCCACGAGACGCTCTACATCTGCGCCACCGACGAGCACGGCA
CGCCGGCTGAACTGGCCGCCGCCGCCGCCGGCCAGGACGTGGCGACCTACTGCGCCGAGCAGCACGTGCTGCAGCACGAG
GTAGGCCGCGCCTTTGGCCTGTCTTGGGATCATTTCGGCCGCTCGTCCTCGCCGCAGAACCATCGCCTGACCCAGCACTT
CTGCCAGGCCCTGGAAGATCACGGCTTGATCGAGGAACGCGTCGACCAGATGGTCTATTCGGTCGACGACAAGCGCTTCC
TGCCCGACCGCTATGTCGAGGGGACCTGCCCGCACTGCAAGTTCGAGAAGGCGCGCGGCGACCAGTGCGACAACTGCGGC
AACCTGCTGGACCCGACCGACCTGATCGATCCCTACTCGGTGATCTCGGGCTCGCGGAACATCGAGGTGCGCGACACCAA
GCACCTCTACCTGCTGCAGACCAAGATGCAGGACAAGATCCGCGCCTGGGTCGACGCCCACGCCGACTGGCCGCCGCTGG
CCCGCTCGATCGCCTACAAGCACCTGGACGAGGGCCTGATCGACCGCGGCATCACCCGTGACCTGGCCTGGGGCATCCCG
GTGGCCCAGGACGGCGTGCCGCGCCCGGGCTTCGAGGAGAAGGTTTTCTACGTCTGGTTCGACGCGCCGATCGAATACAT
CGCCGCCACCCAGGAGTGGGCCGAAGGCTCGCCTGATCGCGACTGGAAGCGCTGGTGGCGCACCGACGCCGGCGCCGACG
ACGTCCGCTACGTCCAGTTCATGGGCAAGGACAATGTGGCGTTCCACACGGTCAGCTTCCCGGCCACGATCCTGGGCTCC
GAAGAGCCGTGGAAGAGCGTCGACATGCTGAAAGCCTTCAACTGGCTGAACTGGTACGGCGGCAAGTTCTCGACCAGCAA
CAAGCGCGGCGTCTTCATGGACGCGGCCCTGGAGATCCTGCCGCCCGACCTGTGGCGCTGGTACCTGACGGCCAACTCGC
CGGAAGGCAGCGACACCGCCTTCACCTGGGAACAGTTCGCCAGCGCCGTGAACCGCGACCTGGCCGATGTGCTGGGCAAT
TTCGTCAACCGCATCCTGAAGTTCAACGAGAGCAAGTTCGAGGGCGTGGTCCCGGCGGGCGGCGAGCCTGGTCCGCTGGA
AGAGAAGCTGTTCGCAGATGTCTCCGCCCGTCTGGCGGATCTGGCCGAACAGATGGACGCCATCGAGATCCGCAAGAGCG
CCCAGGCGCTGCGGGCCCTCTGGGTGGTCGGCAACGAGTATCTGCAGGAAGCCGCGCCCTGGACCGCGATCAAGACCGAT
CGCGACCGCGCCGCCGTCATCGTGCGCACCGCCCTGAACCTGGCGGCCCTCTACGCCAAGATCTCGGCCCCGTTCATTCC
GTTCGCCGCCGAGAAGATCGGCGATGCGTTCGGTCTCGACTTCCCAGCCTCCTGGCCGTCGAACGACGCCAAGGCCGAGC
TCAACACCCTGAGCGTCGGCCGGCCGATCACCGTGCCCGAGGTCCTGTTCAAGAAGATCGAGGACGAGCAGATCGCCGAA
TGGACAGCCCGCTTCGGCGGCGCGGAGTAA

Upstream 100 bases:

>100_bases
CTGTTCGGTCAATCGGCTTGCGCTTTCGTTTCGGGCGCGAGCCTTCTATATGCGGACCCTTCCAAGCGTTCCGTAGACTC
CCCCGAAAGGCCGCGCCCGC

Downstream 100 bases:

>100_bases
GGTGGCGGGCGGCGCGGACAGGACGGAGGGGCAGGGGCTTTCGACCCTGGTTCGCCTGTGCCTGTTCTACGCCGCCATCT
TCCTGAGCTCGGGCGTCAGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAAAAAGQDVATYCAEQHVLQHE
VGRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEERVDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCG
NLLDPTDLIDPYSVISGSRNIEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIP
VAQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQFMGKDNVAFHTVSFPATILGS
EEPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEILPPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGN
FVNRILKFNESKFEGVVPAGGEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTD
RDRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVGRPITVPEVLFKKIEDEQIAE
WTARFGGAE

Sequences:

>Translated_569_residues
MARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAAAAAGQDVATYCAEQHVLQHE
VGRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEERVDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCG
NLLDPTDLIDPYSVISGSRNIEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIP
VAQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQFMGKDNVAFHTVSFPATILGS
EEPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEILPPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGN
FVNRILKFNESKFEGVVPAGGEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTD
RDRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVGRPITVPEVLFKKIEDEQIAE
WTARFGGAE
>Mature_568_residues
ARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAAAAAGQDVATYCAEQHVLQHEV
GRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEERVDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCGN
LLDPTDLIDPYSVISGSRNIEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIPV
AQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQFMGKDNVAFHTVSFPATILGSE
EPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEILPPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGNF
VNRILKFNESKFEGVVPAGGEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTDR
DRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVGRPITVPEVLFKKIEDEQIAEW
TARFGGAE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=573, Percent_Identity=38.3944153577661, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI24308436, Length=548, Percent_Identity=24.2700729927007, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1788432, Length=565, Percent_Identity=30.0884955752212, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17540794, Length=576, Percent_Identity=36.2847222222222, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321704, Length=574, Percent_Identity=36.5853658536585, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321610, Length=552, Percent_Identity=23.3695652173913, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655311, Length=575, Percent_Identity=39.1304347826087, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI21358483, Length=539, Percent_Identity=24.860853432282, Blast_Score=122, Evalue=1e-27,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CAUCN (B8H5U4)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516920.1
- ProteinModelPortal:   B8H5U4
- SMR:   B8H5U4
- GeneID:   7333222
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01547
- OMA:   GSREPWK
- ProtClustDB:   PRK12268
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63727; Mature: 63596

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAA
CCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
AAAGQDVATYCAEQHVLQHEVGRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEER
HHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
VDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCGNLLDPTDLIDPYSVISGSRN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
IEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIP
EEEECCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VAQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQF
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEE
MGKDNVAFHTVSFPATILGSEEPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEIL
ECCCCEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEHHHHHHHC
PPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGNFVNRILKFNESKFEGVVPAG
CCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
RDRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCHHEECCC
RPITVPEVLFKKIEDEQIAEWTARFGGAE
CCCCHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHETLYICATDEHGTPAELAA
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
AAAGQDVATYCAEQHVLQHEVGRAFGLSWDHFGRSSSPQNHRLTQHFCQALEDHGLIEER
HHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
VDQMVYSVDDKRFLPDRYVEGTCPHCKFEKARGDQCDNCGNLLDPTDLIDPYSVISGSRN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
IEVRDTKHLYLLQTKMQDKIRAWVDAHADWPPLARSIAYKHLDEGLIDRGITRDLAWGIP
EEEECCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VAQDGVPRPGFEEKVFYVWFDAPIEYIAATQEWAEGSPDRDWKRWWRTDAGADDVRYVQF
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEE
MGKDNVAFHTVSFPATILGSEEPWKSVDMLKAFNWLNWYGGKFSTSNKRGVFMDAALEIL
ECCCCEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEHHHHHHHC
PPDLWRWYLTANSPEGSDTAFTWEQFASAVNRDLADVLGNFVNRILKFNESKFEGVVPAG
CCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEPGPLEEKLFADVSARLADLAEQMDAIEIRKSAQALRALWVVGNEYLQEAAPWTAIKTD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
RDRAAVIVRTALNLAALYAKISAPFIPFAAEKIGDAFGLDFPASWPSNDAKAELNTLSVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCHHEECCC
RPITVPEVLFKKIEDEQIAEWTARFGGAE
CCCCHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA