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The map label for this gene is dusA [H]

Identifier: 16125555

GI number: 16125555

Start: 1454190

End: 1455215

Strand: Direct

Name: dusA [H]

Synonym: CC_1306

Alternate gene names: 16125555

Gene position: 1454190-1455215 (Clockwise)

Preceding gene: 16125554

Following gene: 16125558

Centisome position: 36.2

GC content: 66.08

Gene sequence:

>1026_bases
GTGGGTAAAGTCCTGAAATATCAAGCTCATAGATTTTCCGTCGCCCCGATGATGGACTGGACCGACCGACACTGTCGGTC
GCTGCACCGCGGGCTGTCGTCGCGGGCGCTGCTCTACACCGAGATGGTGACGAGCGGTGCGGTGGTGCATGGCGATCGAA
ACAAGCTGCTCGGCTATGACCCTGGCCAGCATCCGGTGGCGGTGCAATTGGGCGGGTCGGATCCTGCCGAACTGGCTCAG
GCCGCGCGGATCGCCGAGGACTACGGCTATGACGAGGTGAACCTCAATGTCGGCTGCCCCTCGGATCGGGTGCAAAGCGG
CCGCTTTGGCGCCTGCCTGATGCGCGAACCGGATCTGGTCGCCGAATGTATGGCGGCGATCAAGAACGCTGTACGGGTTC
CCGCCACGGTGAAGTGCCGGATCGGCGTGGATGACCAGGATCCCGAAGAGAGCCTCTTCACGCTGGTCGATCGCTGTGCG
GCGGCGGGGATCGACACCTTTGTTGTTCATGCGCGCAAGGCCTGGCTGCAGGGCCTGTCGCCTAAGGAAAACCGGGACAT
TCCGCCCCTCGACTACGCGCTGGTTTACCGTCTGAAGCGCGAGCGCCCCGCCTTGACCATTGCCATCAATGGCGGTGTGC
CCAGCGTCGATGCGGCGCTGGAGCATCTGGCCAATGGCGTGGACGGCGTGATGCTGGGGCGGGCCGCCTATCATGAGGCG
GGCTTGCTGGGAGAGGTCGACCGGCGCGTGTTCGGGCTGGATGTCGCGGACGTCGACAGCTTCGAGGCCGTCGAGCGCTA
TCGGCCCTATCTGGCGCGGGAACTGGCGGCCGGAACGCATCTGGCGGCAATGAGCCGGCACATGCTGGGCCTTTTCCACG
GCCTGCCCGGCGCCCGGGCCTGGCGCCGTATCCTGACGGTCGAGGGCGTCAAGTCCGGCGCAGGGCTGGACGTAGTGGAT
CGGGCGCTCGACGCCGTTCGCGCCGCGCTGGCGTCTCGAGAAGAACGGTCAGTCCCAGCGGCCTAG

Upstream 100 bases:

>100_bases
ATCCGTGCAAAACCGGCGCGCGGAAGGGGTGCGGGTTGAAGCGTCGCCAGTCTGGCGCGCTTCCTCATGCTCCTAGTATC
GCATAAAGCCTTGTCTCTCC

Downstream 100 bases:

>100_bases
CAGGCTAAGGGTGCAGGATCAGCGAGGTCTGGGTGGCCTCGAAGCGCGAAAGGCGGCCCAGATAGCTCATGCCCAGAAGT
GAGTTTTCCAGGCCCTTCTC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MGKVLKYQAHRFSVAPMMDWTDRHCRSLHRGLSSRALLYTEMVTSGAVVHGDRNKLLGYDPGQHPVAVQLGGSDPAELAQ
AARIAEDYGYDEVNLNVGCPSDRVQSGRFGACLMREPDLVAECMAAIKNAVRVPATVKCRIGVDDQDPEESLFTLVDRCA
AAGIDTFVVHARKAWLQGLSPKENRDIPPLDYALVYRLKRERPALTIAINGGVPSVDAALEHLANGVDGVMLGRAAYHEA
GLLGEVDRRVFGLDVADVDSFEAVERYRPYLARELAAGTHLAAMSRHMLGLFHGLPGARAWRRILTVEGVKSGAGLDVVD
RALDAVRAALASREERSVPAA

Sequences:

>Translated_341_residues
MGKVLKYQAHRFSVAPMMDWTDRHCRSLHRGLSSRALLYTEMVTSGAVVHGDRNKLLGYDPGQHPVAVQLGGSDPAELAQ
AARIAEDYGYDEVNLNVGCPSDRVQSGRFGACLMREPDLVAECMAAIKNAVRVPATVKCRIGVDDQDPEESLFTLVDRCA
AAGIDTFVVHARKAWLQGLSPKENRDIPPLDYALVYRLKRERPALTIAINGGVPSVDAALEHLANGVDGVMLGRAAYHEA
GLLGEVDRRVFGLDVADVDSFEAVERYRPYLARELAAGTHLAAMSRHMLGLFHGLPGARAWRRILTVEGVKSGAGLDVVD
RALDAVRAALASREERSVPAA
>Mature_340_residues
GKVLKYQAHRFSVAPMMDWTDRHCRSLHRGLSSRALLYTEMVTSGAVVHGDRNKLLGYDPGQHPVAVQLGGSDPAELAQA
ARIAEDYGYDEVNLNVGCPSDRVQSGRFGACLMREPDLVAECMAAIKNAVRVPATVKCRIGVDDQDPEESLFTLVDRCAA
AGIDTFVVHARKAWLQGLSPKENRDIPPLDYALVYRLKRERPALTIAINGGVPSVDAALEHLANGVDGVMLGRAAYHEAG
LLGEVDRRVFGLDVADVDSFEAVERYRPYLARELAAGTHLAAMSRHMLGLFHGLPGARAWRRILTVEGVKSGAGLDVVDR
ALDAVRAALASREERSVPAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=28.6343612334802, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40807366, Length=308, Percent_Identity=26.9480519480519, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=317, Percent_Identity=56.782334384858, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1789660, Length=253, Percent_Identity=29.2490118577075, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788462, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=30.5389221556886, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=289, Percent_Identity=24.9134948096886, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24580595, Length=248, Percent_Identity=27.8225806451613, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=248, Percent_Identity=27.8225806451613, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=25.2212389380531, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36984; Mature: 36852

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVLKYQAHRFSVAPMMDWTDRHCRSLHRGLSSRALLYTEMVTSGAVVHGDRNKLLGYD
CCCCEEEHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCC
PGQHPVAVQLGGSDPAELAQAARIAEDYGYDEVNLNVGCPSDRVQSGRFGACLMREPDLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEECCCCHHH
AECMAAIKNAVRVPATVKCRIGVDDQDPEESLFTLVDRCAAAGIDTFVVHARKAWLQGLS
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PKENRDIPPLDYALVYRLKRERPALTIAINGGVPSVDAALEHLANGVDGVMLGRAAYHEA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
GLLGEVDRRVFGLDVADVDSFEAVERYRPYLARELAAGTHLAAMSRHMLGLFHGLPGARA
CCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
WRRILTVEGVKSGAGLDVVDRALDAVRAALASREERSVPAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKVLKYQAHRFSVAPMMDWTDRHCRSLHRGLSSRALLYTEMVTSGAVVHGDRNKLLGYD
CCCEEEHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCC
PGQHPVAVQLGGSDPAELAQAARIAEDYGYDEVNLNVGCPSDRVQSGRFGACLMREPDLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEECCCCHHH
AECMAAIKNAVRVPATVKCRIGVDDQDPEESLFTLVDRCAAAGIDTFVVHARKAWLQGLS
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PKENRDIPPLDYALVYRLKRERPALTIAINGGVPSVDAALEHLANGVDGVMLGRAAYHEA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
GLLGEVDRRVFGLDVADVDSFEAVERYRPYLARELAAGTHLAAMSRHMLGLFHGLPGARA
CCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
WRRILTVEGVKSGAGLDVVDRALDAVRAALASREERSVPAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]