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The map label for this gene is smpB

Identifier: 16125447

GI number: 16125447

Start: 1356442

End: 1356900

Strand: Direct

Name: smpB

Synonym: CC_1196

Alternate gene names: 16125447

Gene position: 1356442-1356900 (Clockwise)

Preceding gene: 16125446

Following gene: 16125452

Centisome position: 33.77

GC content: 65.14

Gene sequence:

>459_bases
ATGTCCAAGCCGATCGCGGAAAATCGGCGCGCGCGCTTCGACTACTTCATCGAGGAGACGTTCGAGGCCGGCATCATGCT
GACCGGCACCGAGGTGAAGAGCCTGCGGACCGGCCGGGCGAACATCGCCGAGTCCTACGCCTCGGTGGAGGGGCGTGAGA
TCGTGCTGATCAACGCCGACATCCCACCCTACGGCCACGCCAACCGATTCAACCACGAACCGCGTCGTCACCGGAAGCTG
CTGCTGCATCGTCGCCAGATCGACAAGCTGATCGGCGCGGTTCAGCGCGAGGGGCGCACCCTGGTGCCGATCAAGCTCTA
TTGGAACGACAAGGGGCTGGCGAAGCTGGAAGTCGGGCTCGCCAAGGGCAAGAAACTGCACGACAAGCGCGACACCGCCG
CCGAACGCGACTGGCAGCGCGACAAGGCCCGCCTGATGAAGGGCGATCGCGGCGACTGA

Upstream 100 bases:

>100_bases
TGGGCCTGTGCACGGAAGATGTGCGCCTGCCGATCACGGCCTGCGCCGAAGGGGTGCGCCCCGCGATCCTTGAGGCCATG
CGCGAGGCTGGTCTGCGCTG

Downstream 100 bases:

>100_bases
GCGAAGTGCGAGCGGCTAAACCGCTCGGAAAGCGCTCCAAGCGTCTGGAAATAGAGCCCTTTTATCCGATCTGATTGAGT
CAGCCAGATCGGAAAGGGCT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINADIPPYGHANRFNHEPRRHRKL
LLHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGLAKGKKLHDKRDTAAERDWQRDKARLMKGDRGD

Sequences:

>Translated_152_residues
MSKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINADIPPYGHANRFNHEPRRHRKL
LLHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGLAKGKKLHDKRDTAAERDWQRDKARLMKGDRGD
>Mature_151_residues
SKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINADIPPYGHANRFNHEPRRHRKLL
LHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGLAKGKKLHDKRDTAAERDWQRDKARLMKGDRGD

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=52.3809523809524, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CAUCN (B8H482)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516627.1
- ProteinModelPortal:   B8H482
- SMR:   B8H482
- GeneID:   7332983
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01254
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17545; Mature: 17414

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINAD
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHCCCHHHHHHHHCCCCCEEEEEECC
IPPYGHANRFNHEPRRHRKLLLHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEH
AKGKKLHDKRDTAAERDWQRDKARLMKGDRGD
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINAD
CCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHCCCHHHHHHHHCCCCCEEEEEECC
IPPYGHANRFNHEPRRHRKLLLHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEH
AKGKKLHDKRDTAAERDWQRDKARLMKGDRGD
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA